Immunohistochemical localization of blood-retinal barrier breakdown sites associated with post-surgical macular oedema

Vinores, Stanley A.; Amin, Abha; Derevjanik, Nancy L.; Green, W. R.; Campochiaro, P. A.

doi:10.1007/bf00158291

Cited by 46 publications

(26 citation statements)

References 37 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…47-49 In humans, postmortem diabetic eyes have localized extravascular albumin at sites of blood-retinal-barrier compromise. 50 Bek examined areas of capillary occlusion in 12 donor eyes with diabetic retinopathy and revealed, in 21 of the 27 studied lesions, an eosinophilic substance between the photoreceptor outer segments and the RPE. 51 More recently, Decanini et al analyzed the RPE proteome in pre-retinopathic diabetic donor eyes and in age-matched control donors.…”

Section: Discussionmentioning

confidence: 99%

Multimodal Characterization of Proliferative Diabetic Retinopathy Reveals Alterations in Outer Retinal Function and Structure

et al. 2015

View full text Add to dashboard Cite

Purpose To identify changes in retinal function and structure in persons with proliferative diabetic retinopathy (PDR), including the effects of panretinal photocoagulation (PRP). Design Cross-sectional study Participants 30 adults who received PRP for PDR, 15 adults with untreated PDR, and 15 age-matched controls Methods Contrast sensitivity, frequency doubling perimetry (FDP), Humphrey visual fields, photostress recovery, and dark adaptation were assessed in all subjects. Fundus photography and macular spectral-domain optical coherence tomography (SD-OCT) were also obtained. SD-OCT scans were semi-automatically segmented to quantify retinal layer thicknesses. Main Outcome Measures Visual function test results were compared between patients with PDR and PRP, untreated patients with PDR, and controls. Mean retinal layer thicknesses were also compared between groups. Correlation analyses were performed to evaluate associations between visual function test results and retinal layer thicknesses. Results Patients with PDR exhibited significant reduction of FDP mean deviation (MD) in PRP-treated (MD ± SD: −8.20 ± 5.76 dB, p<0.0001) and untreated (−5.48 ± 4.48 dB, p<0.0001) patients relative to controls (1.07 ± 2.50 dB). Reduced log contrast sensitivity compared with controls (1.80 ± 0.14) was also observed in both PRP-treated (1.42 ± 0.17, p<0.0001) and untreated (1.56 ± 0.20, p= 0.001) patients with PDR. Compared to controls, patients treated with PRP demonstrated increased photostress recovery time (151.02 ± 104.43 sec vs 70.64 ± 47.14 sec, p=0.001) and dark adaptation speed (12.80 ± 5.15 min vs 9.74 ± 2.56 min, p=0.022) whereas untreated patients had no significant differences in photostress recovery time or dark adaptation speed relative to controls. PRP-treated patients had diffusely thickened nerve fiber layers (p=0.024) and diffusely thinned retinal pigment epithelial layers (RPE) (p=0.009) versus controls. Untreated patients with PDR also had diffusely thinned RPE layers (p=0.031) compared to controls. Conclusions Patients with untreated PDR exhibit inner retinal dysfunction, as evidenced by reduced contrast sensitivity and FDP performance, accompanied by alterations in inner and outer retinal structure. PRP-treated patients had more profound changes in outer retinal structure and function. Distinguishing the effects of PDR and PRP may guide the development of restorative vision therapies for patients with advanced diabetic retinopathy.

show abstract

Section: Discussionmentioning

confidence: 99%

Multimodal Characterization of Proliferative Diabetic Retinopathy Reveals Alterations in Outer Retinal Function and Structure

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In addition, this mild edema with nonspecific area can occur without visual dysfunction. One possible explanation of the cause of postoperative macular edema is damage to the blood–retinal barrier, which could induce consequent macular thickening 20,21…”

Section: Discussionmentioning

confidence: 99%

Retinal thickness changes after phacoemulsification

Pardianto¹,

Moeloek²,

Reveny³

et al. 2013

OPTH

View full text Add to dashboard Cite

PurposeTo determine the effect of phacoemulsification on macular volume and thickness using spectral domain optical coherence tomography examinations.MethodsTwenty-seven eyes of 27 subjects who underwent phacoemulsification were studied. All nine areas of the macula were examined by spectral domain optical coherence tomography preoperatively and 2 months postoperatively. Effective phacoemulsification time and absolute phacoemulsification time were also recorded.ResultsThere were statistically significant differences in macular thickness between preoperative and postoperative spectral domain optical coherence tomography examinations in nine areas including macular volume. In the paracentral macular area, the thickness of three quadrants significantly increased (superior P=0.015; temporal P=0.001; and nasal P=0.023). Peripheral macular thickness also increased significantly in the superior (P=0.05) and temporal macular areas (P<0.001). The macular volume increased significantly after phacoemulsification (P<0.001). There were no correlations between absolute/effective phacoemulsification time and macular cellular structures (P>0.05), but a significant correlation (P=0.011) was found between absolute phacoemulsification time and change in macular volume.ConclusionMacular thickness changes in the nasal, superior, and temporal quadrants of the paracentral area and the superior and temporal quadrants of the peripheral area, as well as macular volume, may be used as detailed biomarkers to measure the effects of intraocular pressure fluctuations and maneuvers in phacoemulsification intraocular surgeries.

show abstract

“…Iodine might serve a similar role also in patients with CME secondary to uveitis, who are thought to have lost integrity of the outer blood-retina barrier and RPE pump, 43 or in patients with CME secondary to diabetic retinopathy, given that dysfunction of the outer blood-retina barrier has been observed in diabetic patients. 44-47 …”

Section: Discussionmentioning

confidence: 99%

The Relationship of Central Foveal Thickness to Urinary Iodine Concentration in Retinitis Pigmentosa With or Without Cystoid Macular Edema

et al. 2014

View full text Add to dashboard Cite

IMPORTANCE Current treatments for cystoid macular edema (CME) in retinitis pigmentosa (RP) are not always effective, may lead to adverse effects, and may not restore visual acuity. The present research lays the rationale for evaluating whether an iodine supplement could reduce CME in RP.OBJECTIVE To determine whether central foveal thickness (CFT) in the presence of CME is related to dietary iodine intake inferred from urinary iodine concentration (UIC) in nonsmoking adults with RP. DESIGN, SETTING, AND PARTICIPANTSWe performed a cross-sectional observational study of 212 nonsmoking patients aged 18 to 69 years referred to our institution for RP with visual acuity of no worse than 20/200 in at least 1 eye. EXPOSURE Retinitis pigmentosa with or without CME. MAIN OUTCOMES AND MEASURESWith the eye as the unit of analysis, the relationship of log CFT measured by optical coherence tomography to UIC measured from multiple spot samples and represented as a 3-level classification variable (<100, 100-199, and Ն200 μg/L), assigning greater weight to patients with more reliable UIC estimates. RESULTSAnalyses were limited to 199 patients after excluding 11 who failed to return urine samples for measuring UIC and 2 outliers for UIC. Of the 199 patients, 36.2% had CME in 1 or both eyes. Although log CFT was inversely related to UIC based on findings from all eyes (P = .02), regression of log CFT on UIC separately for eyes with and without CME showed a strong inverse significant relationship for the former group (P < .001) and no significant relationship for the latter group (P = .66) as tested. For the eyes with CME, CFT ranged from a geometric mean of 267 μm for a median UIC of less than 100 μg/L to a geometric mean of 172 μm for a median UIC of 200 μg/L or greater. In contrast, we found no significant association between CME prevalence and UIC based on the entire sample as tested (odds ratio, 1.01 [95% CI, 0.38-2.67]; P = .99).CONCLUSIONS AND RELEVANCE A higher UIC in nonsmoking adults with RP was significantly associated with less central foveal swelling in eyes with CME. Additional study is required to determine whether an iodine supplement can limit or reduce the extent of CME in patients with RP.

show abstract

Immunohistochemical localization of blood-retinal barrier breakdown sites associated with post-surgical macular oedema

Cited by 46 publications

References 37 publications

Multimodal Characterization of Proliferative Diabetic Retinopathy Reveals Alterations in Outer Retinal Function and Structure

Multimodal Characterization of Proliferative Diabetic Retinopathy Reveals Alterations in Outer Retinal Function and Structure

Retinal thickness changes after phacoemulsification

The Relationship of Central Foveal Thickness to Urinary Iodine Concentration in Retinitis Pigmentosa With or Without Cystoid Macular Edema

Contact Info

Product

Resources

About