Immunohistochemical localization of adenosine deaminase in human benign extrathymic lymphoid tissues and b-cell lymphomas

Abstract

Immunomorphologic methods were utilized to localize adenosine deaminase (ADA) in extrathymic benign lymphoid tissues and B‐cell lymphomas. In reactive lymph nodes, tonsils and appendix, germinal centers displayed strong ADA‐positive nuclear staining in small cleaved lymphocytes and weak nuclear and/or cytoplasmic staining in large lymphoid cells. A significant proportion of ADA‐positive lymphocytes in the germinal centers were B‐cells. The mantle zone of secondary follicles did not stain for ADA. The plasma cells in the medullary cords demonstrated mainly cytoplasmic staining. In the spleen, ADA‐positive lymphocytes were located in the periarteriolar sheath and paratrabecular white pulp. In lymphoma B‐cells, patterns of ADA staining were similar to those observed in normal B‐lymphocytes of similar morphology. This study demonstrated that human normal and lymphoma B‐lymphoid cells are heterogeneous with respect to ADA expression. This heterogeneity appears to be associated with differentiation and/or proliferation of B‐lymphocytes. Cancer 53:70‐78, 1984.

Anti-adenosine deaminase antibodies in lupus erythematosus

“…Past reports indicated the detection of ADA activity was primarily from cytosol fractions of organs and tissues. [25][26][27][28][29] ADA was found in cytoplasm as well as nuclei of cells using polyclonal anti-ADA antibodies in immunohistochemistry in a series of studies by Chechik et al [30][31][32][33][34] This group showed nuclear stain-ing of Molt-4 lymphoblast cells and most of lymphocytes in thymus cortex, but not in the medulla, 31 and also described cytoplasmic staining using the same polyclonal anti-ADA antibody on ethanol -acetic acid…”

“…33 Using a similar approach, Chechik et al reported the cytoplasmic and nuclear staining on plasmacytoma cells, and predominantly nuclear staining on lymphoma cells. 30,32 This group subsequently showed evidence to suggest that the patterns of expression of ADA might be associated with differentiation and proliferation of cells. 34 Our IIF study with puri ed anti-ADA revealed discrete speckled nuclear staining patterns on acetone-xed HEp-2 cells, con rming that ADA may be localized in nuclei.…”

“…34 Our IIF study with puri ed anti-ADA revealed discrete speckled nuclear staining patterns on acetone-xed HEp-2 cells, con rming that ADA may be localized in nuclei. [30][31][32][33][34] There is a possibility that nuclear localization of ADA in our study was caused by contamination of anti-snRNP antibodies, although we consider it unlikely. Our puri ed anti-ADA antibody did not precipitate any RNA in immunoprecipitation using P 32 labeled HEp-2 cell extract as antigenic source (data not shown here).…”

“…Our puri ed anti-ADA antibody did not precipitate any RNA in immunoprecipitation using P 32 labeled HEp-2 cell extract as antigenic source (data not shown here). In addition to nuclear location, [30][31][32][33][34] ADA was detected on the cell surface of HEp-2 cells and on mononuclear cells. [6][7][8][9][10] Thus, ADA appears to belong to the group of nuclear proteins showing dual localizations of nuclei and cell membrane, including histones, DNA, Ku antigen, SSB=La, SSA=Ro, and nucleolin.…”