Immunohistochemical Localization of Adenosine Deaminase in Human Lymphoid Tissues and Lymphomas

Chechik, Boris E.; Schrader, William P.; Perets, Ann; Fernandes, J.

doi:10.1007/978-1-4757-0390-0_49

Cited by 1 publication

(4 citation statements)

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“…Past reports indicated the detection of ADA activity was primarily from cytosol fractions of organs and tissues. [25][26][27][28][29] ADA was found in cytoplasm as well as nuclei of cells using polyclonal anti-ADA antibodies in immunohistochemistry in a series of studies by Chechik et al [30][31][32][33][34] This group showed nuclear stain-ing of Molt-4 lymphoblast cells and most of lymphocytes in thymus cortex, but not in the medulla, 31 and also described cytoplasmic staining using the same polyclonal anti-ADA antibody on ethanol -acetic acid…”

Section: Discussionmentioning

confidence: 99%

“…33 Using a similar approach, Chechik et al reported the cytoplasmic and nuclear staining on plasmacytoma cells, and predominantly nuclear staining on lymphoma cells. 30,32 This group subsequently showed evidence to suggest that the patterns of expression of ADA might be associated with differentiation and proliferation of cells. 34 Our IIF study with puri ed anti-ADA revealed discrete speckled nuclear staining patterns on acetone-xed HEp-2 cells, con rming that ADA may be localized in nuclei.…”

Section: Discussionmentioning

confidence: 99%

“…34 Our IIF study with puri ed anti-ADA revealed discrete speckled nuclear staining patterns on acetone-xed HEp-2 cells, con rming that ADA may be localized in nuclei. [30][31][32][33][34] There is a possibility that nuclear localization of ADA in our study was caused by contamination of anti-snRNP antibodies, although we consider it unlikely. Our puri ed anti-ADA antibody did not precipitate any RNA in immunoprecipitation using P 32 labeled HEp-2 cell extract as antigenic source (data not shown here).…”

Section: Discussionmentioning

confidence: 99%

“…Our puri ed anti-ADA antibody did not precipitate any RNA in immunoprecipitation using P 32 labeled HEp-2 cell extract as antigenic source (data not shown here). In addition to nuclear location, [30][31][32][33][34] ADA was detected on the cell surface of HEp-2 cells and on mononuclear cells. [6][7][8][9][10] Thus, ADA appears to belong to the group of nuclear proteins showing dual localizations of nuclei and cell membrane, including histones, DNA, Ku antigen, SSB=La, SSA=Ro, and nucleolin.…”

Section: Discussionmentioning

confidence: 99%

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Anti-adenosine deaminase antibodies in lupus erythematosus

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2002

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Adenosine deaminase (ADA) is an enzyme involved in purine metabolism and has a major role in the development and function of lymphoid cells. Congenital deficiency of ADA results in severe immunodeficiency. Patients with congenital ADA deficiency treated with polyethylene glycol-conjugated bovine ADA develop antibodies to ADA. This leads us to investigate the role of anti-ADA antibodies in patients with systemic rheumatic diseases. Commercially available ADA was used in ELISA and immunoblots for detection of anti-ADA antibodies. Four out of 100 patients examined were positive for anti-ADA antibodies. Two of them had peripheral blood lymphopenia but the antibody levels did not appear to correlate with the lymphocyte counts. Immunoblotting revealed that the antibodies recognized a 40 kDa peptide of ADA, corresponding to ADA1, the major component of ADA. Affinity-purified antibodies were used to locate the distribution of ADA on Hep-2 cells and lymphocytes by indirect immunofluorescence. Anti-ADA antibodies gave a distinct nuclear speckled pattern on acetone-fixed cells. With viable cell immunofluorescence, anti-ADA antibodies also stained the cell surface of HEp-2 cells and lymphocytes, indicating surface expression of ADA. The anti-ADA antibodies failed to gain access into the cytoplasm or nuclei when added to the cultures of HEp-2 cells. In summary, this is the first report of detection of anti-ADA1 autoantibody which is a new type of ANA with discrete, speckled nuclear staining, but which may not be associated with lymphopenia.

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