Immunohistochemical localization, identification and regulation of the interleukin-1 receptor antagonist in the human endometrium

Simón, Carlos; Frances, Ana; Lee, Bo Yon; Mercader, Amparo; Huynh, Tri H. V.; Remohı́, J.; Polan, Mary Lake; Pellicer, A.

doi:10.1093/molehr/1.7.324

Cited by 21 publications

(22 citation statements)

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“…IL-1ra's possible protective effect becomes even more likely in the context that IL1ra was not only present in all secretory phase samples and the one investigated sample with an intrauterine pregnancy but did not occur in anyone of the 3 tested 340 fallopian tubes with an extrauterine pregnancy. This hypothesis is furthermore supported by findings of other groups describing a decrease in IL-1ra expression in the human endometrium at the time of implantation to possibly facilitate the embryos invasion into the maternal endometrium (Simon et al, 1995). Another study showed increased levels of IL-1ra in endometrial stromal cells of the secretory phase (Fukuda 345 et al, 1995).…”

Section: Interleukin-1 System In Human Fallopian Tube Page 18supporting

confidence: 65%

Interleukin-1 system in the human fallopian tube—No spatial but a temporal regulation of mRNA and protein expression

Hess

Baston-Buest

Schanz

et al. 2009

Molecular and Cellular Endocrinology

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, et al.. Interleukin-1 system in the human fallopian tube -no spatial but a temporal regulation of mRNA and protein expression. Molecular and Cellular Endocrinology, Elsevier, 2009, 303 (1-2) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 AbstractThe human fallopian tube provides the environment for the first 5 days of embryonic development in vivo. The IL-1 system is involved in human embryo implantation.This study aimed to investigate IL-1, IL-1ra and IL-1R tI expression within the 25 length of the human fallopian tube on mRNA-and protein-level in samples from proliferative versus secretory phase, postmenopause samples (PMP) and samples from intra-(IUP) and extrauterine (EUP) pregnancies to examine possible spatial and hormonal induced changes (fimbrial, ampullary and isthmic tube segments). On mRNA-level, IL-1β was expressed in all samples except in PMP. IL-1R tI could be 30 detected in all samples whereas IL-1ra was only expressed in secretory phase and the IUP sample. Immunohistochemically we could detect IL-1β and IL-1R t1 protein in all proliferative and secretory phase samples with maximum intensity in secretory phase samples whereas IL-1ra was expressed in secretory phase samples only.Overall no spatial but temporal differences possibly due to hormonal changes could 35 be observed suggesting a precise regulation of the IL-1 system, especially for IL-1ra and moreover a stable molecular architecture within the full length of the fallopian tube.

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Section: Interleukin-1 System In Human Fallopian Tube Page 18supporting

confidence: 65%

Interleukin-1 system in the human fallopian tube—No spatial but a temporal regulation of mRNA and protein expression

Hess

Baston-Buest

Schanz

et al. 2009

Molecular and Cellular Endocrinology

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“…A decline in glandular IL-1Ra levels coincides with the "window of implantation." 40 Observations in our laboratory suggest that the resulting free IL-1␤ enhances expression of several monocyte recruiting and activating chemokines in decidual cells that promote a physiological monocyte influx during early gestation but is normally transient because extravillous cytotrophoblasts release IL-1Ra. 41,42 However, increased expression of IL-1␤ in significant subsets of preeclampsia cases accompanying maternal infections, such as Chlamydia 43,44 and viruses, 45,46 and other inflammatory states may overwhelm this block of the IL-1R by IL-1Ra.…”

Section: Discussionmentioning

confidence: 91%

The Implication of Aberrant GM-CSF Expression in Decidual Cells in the Pathogenesis of Preeclampsia

Huang

Zenclussen

Chen

et al. 2010

The American Journal of Pathology

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Preeclampsia is characterized by an exaggerated systemic inflammatory state as well as shallow placentation. In the decidual implantation site , preeclampsia is accompanied by an excessive number of both macrophages and dendritic cells as well as their recruiting chemokines, which have been implicated in the impairment of endovascular trophoblast invasion. Granulocyte-macrophage colony-stimulating factor is known to regulate the differentiation of both macrophages and dendritic cells, prompting both in vivo and in vitro evaluation of granulocyte-macrophage colony-stimulating factor expression in human decidua as well as in a mouse model of preeclampsia. This study revealed increased granulocyte-macrophage colony-stimulating factor expression levels in preeclamptic decidua. Moreover, both tumor necrosis factor-␣ and interleukin-1 ␤, cytokines that are implicated in the genesis of preeclampsia, markedly up-regulated granulocyte-macrophage colony-stimulating factor production in cultured first-trimester human decidual cells. The conditioned media of these cultures promoted the differentiation of both macrophages and dendritic cells from a monocyte precursor. Evaluation of a murine model of preeclampsia revealed that the decidua of affected animals displayed higher levels of immunoreactive granulocyte-macrophage colony-stimulating factor as well as increased numbers of both macrophages and dendritic cells when compared to control animals. Because granulocyte-macrophage colony-stimulating factor is a potent inducer of differentiation and activation of both macrophages and dendritic cells , these findings suggest that this factor plays a crucial role in the pathogenesis of preeclampsia. (Am J

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“…TGF-ß, may be the cause of the discrepancies between immunoassay and bioassay values of IL-1. IL-1ra may well be produced by endometrial cells as by other cells cultured [29,30]. MPS cells as well as many other cell types are known to produce TGF-ß, a substance known to be an effective inhibitor of IL-1 bioactivity [31].…”

Section: Discussionmentioning

confidence: 99%

Production of Interleukins 1β, 6 and 8 and Tumor Necrosis Factor Alpha in Separated and Cultured Endometrial and Endometriotic Stromal and Epithelial Cells

Bergqvist¹,

Nejaty²,

Fröysa³

et al. 2000

Gynecol Obstet Invest

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The production of IL-1β, IL-6, IL-8 and TNF-α was studied in short-time culture of separated stromal and epithelial cells. The cytokine secretion into culture medium was analyzed using immunoassay to evaluate the cytokine protein levels and bioassay to assess the bioactivity of the cytokines. Tissue samples of endometrium and ovarian endometriomas were obtained from 4 patients operated on for clinical reasons. Only IL-8 was found in all samples. IL-1β and TNF-α were detected in the culture medium from most stromal cell samples, but in fewer media from epithelial cell samples. IL-6 was measurable in a few medium samples. Few of the samples displayed a bioactivity. There was no obvious difference between endometrium and endometriotic cell samples besides the production of IL-8 that seems to be lower in endometriotic tissue.

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Immunohistochemical localization, identification and regulation of the interleukin-1 receptor antagonist in the human endometrium

Cited by 21 publications

References 0 publications

Interleukin-1 system in the human fallopian tube—No spatial but a temporal regulation of mRNA and protein expression

Interleukin-1 system in the human fallopian tube—No spatial but a temporal regulation of mRNA and protein expression

The Implication of Aberrant GM-CSF Expression in Decidual Cells in the Pathogenesis of Preeclampsia

Production of Interleukins 1β, 6 and 8 and Tumor Necrosis Factor Alpha in Separated and Cultured Endometrial and Endometriotic Stromal and Epithelial Cells

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