Immunohistochemical Labeling of the Inhibin/Activin βC Subunit in Normal Human Placental Tissue and Chorionic Carcinoma Cell Lines

Weißenbacher, Tobias; Brüning, Ansgar; Kimmich, Tanja; Makovitzky, Josef; Gingelmaier, Andrea; Mylonas, Ioannis

doi:10.1369/jhc.2010.956185

Cited by 9 publications

(15 citation statements)

References 49 publications

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“…Concerning activins B and AB, conflicting studies about their expression in placenta were reported (McCluggage et al, 1998;Petraglia, 1997). The βc and βE subunits were also identified in this organ (Gingelmaier et al, 2011;Weissenbacher et al, 2010).…”

Section: Inhibins and Activinsmentioning

confidence: 99%

The endocrine function of human placenta: an overview

Costa

2016

Reproductive BioMedicine Online

261

173

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During pregnancy, several tightly coordinated and regulated processes take place to enable proper fetal development and gestational success. The formation and development of the placenta is one of these critical pregnancy events. This organ plays essential roles during gestation, including fetal nourishment, support and protection, gas exchange and production of several hormones and other mediators. Placental hormones are mainly secreted by the syncytiotrophoblast, in a highly and tightly regulated way. These hormones are important for pregnancy establishment and maintenance, exerting autocrine and paracrine effects that regulate decidualization, placental development, angiogenesis, endometrial receptivity, embryo implantation, immunotolerance and fetal development. In addition, because they are released into maternal circulation, the profile of their blood levels throughout pregnancy has been the target of intense research towards finding potential robust and reliable biomarkers to predict and diagnose pregnancy-associated complications. In fact, altered levels of these hormones have been associated with some pathologies, such as chromosomal anomalies or pre-eclampsia. This review proposes to revise and update the main pregnancy-related hormones, addressing their major characteristics, molecular targets, function throughout pregnancy, regulators of their expression and their potential clinical interest.

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Section: Inhibins and Activinsmentioning

confidence: 99%

The endocrine function of human placenta: an overview

Costa

2016

Reproductive BioMedicine Online

261

173

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“…Immunofluorescence analysis was performed as previously described (9,(11)(12)(13)(14)(15)23,25). Briefly, cells grown on glass coverslips were fixed with acetone for 10 min at room temperature and washed twice with PBS.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, two additional β-subunits have been identified in humans, βC and βE (7). Although these novel subunits are synthesised in a wide range of normal and malignant tissues (9)(10)(11)(12)(13)(14)(15), their precise function still remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Inhibin-α subunit in normal and malignant human cervical tissue and cervical cancer cell lines

Mylonas

Dian²

2011

Oncol Rep

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Abstract. Inhibins are dimeric glycoproteins composed of an α-subunit and one of two possible β-subunits (βA or βB), with substantial roles in human reproduction and in endocrineresponsive tumours. Four normal cervical tissue samples together with 10 specimens of well-differentiated squamous cervical cancer and adenocarcinoma of the cervix were immunohistochemically analysed for the expression of the inhibin-α subunit. Additionally, two cervical carcinoma cell lines (HeLa and CaSKi) were analysed with immunofluorescence and RT-PCR for the expression of the inhibin-α subunit. We demonstrated for the first time an immunolabelling of the inhibin-α subunit in normal and malignant cervical tissue, as well as cervical cancer cells. However, the immunoreactive reaction was albeit weak and mostly confined to mitotic cells in the analysed cervical tissues. Additionally, the expression pattern of this subunit was rather inconsistent. Therefore, the immunohistochemical evaluation of this subunit in cervical tissue cannot be used as a prognostic marker as suggested for endometrial cancer. However, since the expression of the inhibin-α subunit is minimal in HeLa cells as assessed by immunofluorescence and RT-PCR, the CaSKi cell line might be a better model for further functional experiments regarding cervical pathogenesis.

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“…Since specific antibodies against this inhibin-ßC subunit have been available for just a short period of time, systematic investigations on cervical tissue has not been performed yet. Although the used polyclonal inhibin-ßC antibody is not suitable for immunoblot analysis, it has been successfully used in human placental and endometrial tissue as well as cancer cell lines with a good specificity as confirmed by RT-PCR analysis (12,13,15,30). Therefore, the aim of this study was to analyse the expression of the ßC-subunits in normal and pathological cervical tissue as well as cervical carcinoma cell lines.…”

Section: Immunolabelling Of the Inhibin/activin-ßc Subunit In Normal mentioning

confidence: 99%

“…Additionally, two additional ß-subunits have been identified in humans, determined as ßC and ßE (7). Although these novel subunits are synthesised in wide range of normal and malignant tissues (11)(12)(13)(14)(15), their precise function remains unclear. Moreover, the ßC-isoform is involved in the formation of homodimeric activin C (ßC-ßC) as well as heterodimeric activins AC (ßA-ßC), BC (ßB-ßC), CE (ßC-ßE), as well as inhibin C (•-ßC) have been demonstrated (16,17).…”

Section: Introductionmentioning

confidence: 99%

Immunolabelling of the inhibin/activin-βC subunit in normal and malignant human uterine cervical tissue and cervical cancer cell lines

Blankenstein

Jückstöck²,

Shabani³

et al. 2010

Oncol Rep

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Inhibins are dimeric glycoproteins, composed of an •-subunit and one of two possible ß-subunits (ßA or ßB), with substantial roles in human reproduction and in endocrineresponsive tumours. Recently a novel ß subunit named ßC was described, although it is still unclear if normal or cancerous cervical epithelial cells as well as cervical cancer cell lines can synthesise the inhibin-ßC subunit. Four normal cervical tissue samples together with specimens of welldifferentiated squamous cervical cancer and adenocarcinoma of the cervix were immunohistochemically analyzed. Additionally, two cervical carcinoma cell lines (HeLa and CaSKi) were analyzed by immunofluorescence for the expression of this novel subunit. We demonstrated for the first time an immunolabelling of the inhibin-ßC subunit in normal and malignant cervical tissue, as well as cervical cancer cells. Although the physiological role is still unclear in cervical tissue, the inhibin-ßC subunit might play important roles in carcinogenesis. Moreover, the synthesis of this subunit in cervical carcinoma cell lines of squamous and epithelial origins allows the use of these cell lines in elucidating its functions in cervical pathogenesis.

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Immunohistochemical Labeling of the Inhibin/Activin βC Subunit in Normal Human Placental Tissue and Chorionic Carcinoma Cell Lines

Cited by 9 publications

References 49 publications

The endocrine function of human placenta: an overview

The endocrine function of human placenta: an overview

Inhibin-α subunit in normal and malignant human cervical tissue and cervical cancer cell lines

Immunolabelling of the inhibin/activin-βC subunit in normal and malignant human uterine cervical tissue and cervical cancer cell lines

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