Immunohistochemical expression of types I and III collagen antibodies in the temporomandibular joint disc of human foetuses

Lodi, Fábio Redivo; Gomes, Thiago Simão; Marques, Sérgio Ricardo; Oshima, Celina T. F.; Lancellotti, Carmen Lúcia Penteado; Rodríguez‐Vázquez, José Francisco; Alonso, Luís Garcia; Moraes, Luís Otávio Carvalho de

doi:10.4081/ejh.2011.e24

Cited by 14 publications

(17 citation statements)

References 16 publications

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“…42-44 However, characteristics of condylar (secondary) cartilage that have been identified and documented in rodents have not been assessed in human fetuses.…”

Section: Introductionmentioning

confidence: 99%

“…In human fetuses, structural features of the TMJ, including condylar cartilage, are well studied; 36 - 41 moreover, immunohistochemical analyses have been performed on the articular disc. 42 - 44 However, characteristics of condylar (secondary) cartilage that have been identified and documented in rodents have not been assessed in human fetuses.…”

Section: Introductionmentioning

confidence: 99%

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An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses

et al. 2013

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Immunohistochemical localization of collagen types I, II, and X, aggrecan, versican, dentin matrix protein (DMP)-1, martix extracellular phosphoprotein (MEPE) were performed for Meckel’s cartilage, cranial base cartilage, and mandibular condylar cartilage in human midterm fetuses; staining patterns within the condylar cartilage were compared to those within other cartilaginous structures. Mandibular condylar cartilage contained aggrecan; it also had more type I collagen and a thicker hypertrophic cell layer than the other two types of cartilage; these three characteristics are similar to those of the secondary cartilage of rodents. MEPE immunoreactivity was first evident in the cartilage matrix of all types of cartilage in the human fetuses and in Meckel’s cartilage of mice and rats. MEPE immunoreactivity was enhanced in the deep layer of the hypertrophic cell layer and in the cartilaginous core of the bone trabeculae in the primary spongiosa. These results indicated that MEPE is a component of cartilage matrix and may be involved in cartilage mineralization. DMP-1 immunoreactivity first became evident in human bone lacunae walls and canaliculi; this pattern of expression was comparable to the pattern seen in rodents. In addition, chondroid bone was evident in the mandibular (glenoid) fossa of the temporal bone, and it had aggrecan, collagen types I and X, MEPE, and DMP-1 immunoreactivity; these findings indicated that chondroid bone in this region has phenotypic expression indicative of both hypertrophic chondrocytes and osteocytes.

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“…42-44 However, characteristics of condylar (secondary) cartilage that have been identified and documented in rodents have not been assessed in human fetuses.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses

et al. 2013

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“…The temporomandibular joint is the only synovial joint in the body in which its bone surfaces are covered by a layer of dense connective tissue rather than by a hyaline (articular) cartilage, 1 the same connective tissue that constitutes the temporomandibular articular disc. 2 Early in embryonic development, however, endochondral ossification starts into a hyaline cartilage that arises from a condylar blastema. Therefore, as in other parts of the body where that process takes place, chondrocytes proliferate, become hypertrophied and then undergo apoptosis, providing a condition in which the cartilaginous matrix mineralizes.…”

Section: Introductionmentioning

confidence: 99%

Effect of alendronate on endochondral ossification in mandibular condyles of growing rats

Bradaschia-Corrêa¹,

Barrence

Ferreira³

et al. 2012

Eur J Histochem

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The replacement of the calcified cartilage by bone tissue during the endochondral ossification of the mandibular condyle is dependent of the resorbing activity of osteoclats. After partial resorption, calcified cartilage septa are covered by a primary bone matrix secreted by osteoblasts. Osteoadherin (OSAD) is a small proteoglycan present in bone matrix but absent in cartilage during the endochondral ossification. The aim of this study was to analyze the effect of alendronate, a drug known to inhibit bone resorption by osteoclasts, on the endochondral ossification of the mandibular condyle of young rats, by evaluating the distribution of osteoclasts and the presence of OSAD in the bone matrix deposited. Wistar newborn rats (n=45) received daily injections of alendronate (n=27) or sterile saline solution as control (n=18) from the day of birth until the ages of 4, 14 and 30 days. At the days mentioned, the mandibular condyles were collected and processed for transmission electron microscopy analysis. Specimens were also submitted to tartrate resistant acid phosphatase (TRAP) histochemistry and ultrastructural immunodetection of OSAD. Alendronate treatment did not impede the recruitment and fusion of osteoclasts at the ossification zone during condyle growth, but they presented inactivated phenotype. The trabeculae at the ossification area consisted of cartilage matrix covered by a layer of primary bone matrix that was immunopositive to OSAD at all time points studied. Apparently, alendronate impeded the removal of calcified cartilage and maturation of bone trabeculae in the mandibular ramus, while in controls they occurred normally. These findings highlight for giving attention to the potential side-effects of bisphosphonates administered to young patients once it may represent a risk of disturbing maxillofacial development.

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“…Structural features of TMJ are well studied in the clinical field of oral surgery. 1–3 The mandibular condyle is a member of TMJ and composed of cartilage and fibrous layers covering the surface of the joint. 4 The condylar cartilage also works as a growth cartilage in mandible, and development and growth of this cartilage is important in the clinical field of orthodontics.…”

Section: Introductionmentioning

confidence: 99%

An in situ hybridization study of the insulin-like growth factor system in developing condylar cartilage of the fetal mouse mandible

et al. 2012

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The objective of this study was to investigate the involvement of the insulin-like growth factor (IGF) system in the developing mandibular condylar cartilage and temporomandibular joint (TMJ). Fetal mice at embryonic day (E) 13.0–18.5 were used for in situ hybridization studies using [35S]-labeled RNA probes for IGF-I, IGF-II, IGF-I receptor (-IR), and IGF binding proteins (-BPs). At E13.0, IGF-I and IGF-II mRNA were expressed in the mesenchyme around the mandibular bone, but IGF-IR mRNA was not expressed within the bone. At E14.0, IGF-I and IGF-I mRNA were expressed in the outer layer of the condylar anlage, and IGF-IR mRNA was first detected within the condylar anlage, suggesting that the presence of IGF-IR mRNA in an IGF-rich environment triggers the initial formation of the condylar cartilage. IGFBP-4 mRNA was expressed in the anlagen of the articular disc and lower joint cavity from E15.0 to 18.5. When the upper joint cavity was formed at E18.5, IGFBP-4 mRNA expression was reduced in the fibrous mesenchymal tissue facing the upper joint cavity. Enhanced IGFBP-2 mRNA expression was first recognized in the anlagen of both the articular disc and lower joint cavity at E16.0 and continued expression in these tissues as well as in the fibrous mesenchymal tissue facing the upper joint cavity was observed at E18.5. IGFBP-5 mRNA was continuously expressed in the outer layer of the perichondrium/fibrous cell layer in the developing mandibular condyle. These findings suggest that the IGF system is involved in the formation of the condylar cartilage as well as in the TMJ.

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Immunohistochemical expression of types I and III collagen antibodies in the temporomandibular joint disc of human foetuses

Cited by 14 publications

References 16 publications

An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses

An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses

Effect of alendronate on endochondral ossification in mandibular condyles of growing rats

An in situ hybridization study of the insulin-like growth factor system in developing condylar cartilage of the fetal mouse mandible

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