Immunohistochemical expression of TLR-4 in temporomandibular joint dysfunction

Almeida, Luís Eduardo; Hresko, Kyle; Sorenson, Adam; Butcher, Seth; Tayebi, Lobat; Leonardi, Rosalia; Loreto, Carla; Bósio, José A.; Camejo, Flavio de Alcântara; Doetzer, Andrea Duarte

doi:10.1080/08869634.2018.1446770

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…18 Several studies mentioned that NFκB is the main inflammatory mediator in rheumatoid arthritis and cancer, cervical cancer, nasopharyngeal carcinoma, periapical lesions and TMJ osteoarthritis. [19][20][21][22][23] Activation of NFκB in the synovial layer also occurred among mice that had synovitis caused by a mechanical stress response due to excessive pressure. 24 NFκB expression was shown to be higher on the 7 th and 14 th days and was at its highest on the 21 st day.…”

Section: Discussionmentioning

confidence: 99%

NFkB and MMP-13 expression in condylar cartilage of temporomandibular joint with occlusal disharmony in vivo

Suhartini,

Narmada,

Hamzah

et al. 2024

Dent. J.

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Background: Temporomandibular disorder (TMD) is a collection of symptoms that causes pain and disturbs a person's life quality. One of the trigger factors is mechanical overloading. Mechanical overloading in occlusal disharmony conditions will lead to an inflammatory reaction in the temporomandibular joint (TMJ). This condition will induce nuclear factor Kappa Beta (NFkB) activation to transcribe proinflammatory cytokines and matrix metalloproteinase-13 (MMP13) and will also degrade condylar cartilage as a major factor in strengthening the TMJ. Purpose: The aim of this study was to analyze the expression of NFkB and MMP13 in the condylar cartilage of TMJ with occlusal disharmony. Methods: This research was an experimental study with post-test-only control group design. Twenty Rats (Rattus norvegicus) were divided into four groups: One control group without any intervention and three experimental groups. Occlusal-reducing intervention was due on the right molar of the experimental groups, which were divided into the 7th, 14th, and 21st days. Immunohistochemical staining was performed to determine the expression of NFkB and MMP13 in the condylar cartilage. The data were analyzed by using the Welch test and independent t-test. Results: There were significant differences in NFkB and MMP13 expression between the control and experiment experimental groups (p<0.05). NFkB expression increased on the 7th, 14th, and 21st days of observation. The MMP13 expression showed a significant difference between the control and experimental groups (p<0.05). Conclusion: Occlusal disharmony increases NFkB and MMP13 expression and could affect TMJ integrity and induce TMD. These findings are important for describing the mechanism of TMJ damage and developing potential alternative therapies to prevent further TMD.

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Section: Discussionmentioning

confidence: 99%

NFkB and MMP-13 expression in condylar cartilage of temporomandibular joint with occlusal disharmony in vivo

Suhartini,

Narmada,

Hamzah

et al. 2024

Dent. J.

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“…TLR-4 is known to participate in carcinogenesis, inflammation, and increased levels of TLR-4, TLR-5 and IRF4 were proposed as diagnostic markers of knee osteoarthritis [25] and joint dysfunction [26]. In keratinocytes and epithelium, as well as in other connective tissue cells, in places of chronic and acute inflammation, under the influence of NFkB, the synthesis of HIF1 and VEGF/VEGFR pathway can be activated via the TLR-5-NOX4-ROS signaling pathway [20] [21] The decrease in the level of monocytes in the experimental group may be caused by non-specific reasons, such as an infectious or inflammatory process, autoimmune pathology, prolonged hypoxia.…”

Section: Discussionmentioning

confidence: 99%

“…FGFs are known to be involved in the regulation of proliferation and differentiation of osteoblasts and fibroblasts, in the processes of angiogenesis and tissue regeneration, they activate osteoblast differentiation and inhibit osteoclast differentiation. Individual FGF fractions positively regulate bone growth and density [25] [26]. An increased level of FGF stimulates angiogenesis in the cartilage tissue, so this is one of the possible causes of the formation of defective cartilage.…”

Section: Discussionmentioning

confidence: 99%

Innate Immunity in the Development of Connective Tissue Dysplasia: Pilot Study in Children with Congenital Hip Dislocation

Yazykova¹,

Musikhina²,

Vlasov³

et al. 2022

JBM

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Introduction: Observing and treatment of hip dysplasia in children have always been in the sphere of interest of modern molecular medicine. The role of molecular factors in the formation of connective tissue dysplasia in children is considered crucial for such multisystem disorders, and connective tissue dysplasia progressing involves immune system parameters and biochemical markers. The aim of this work was to establish the relationship between immune status indicators and biochemical markers of connective tissue using bioinformatics and modeling methods. Materials and Methods: 27 patients with congenital hip dislocation, admitted to the University Clinic of Privolzhsky Research Medical University, Department of children orthopedics for surgical treatment, were examined. Determination of 10 blood parameters was conducted by modern biochemical and immunological methods. Statistica 12.0 software from StatSoft was used for statistical data processing. Methods of nonparametric statistics were used since the samples in the control group partially follow the normal distribution. Correlation methods and regression modeling methods were used to evaluate the relationship of indicators. Results and Conclusion: In our investigation we have shown the presence of statistical and mathematical interactions between the parameters of innate immunity and indicators of connective tissue metabolism. The leading role of the immune system in the development of pathologies associated with connective tissue dysplasia is assumed. In further investigations it is necessary to clarify the role hypoxia in HIF-1 stimulated control of skeletal dysplasia, collagen modification, connective tissue dysplasia development.

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“…It can participate in the development of multiple diseases by specifically activating autoimmune responses. [14] Located at 9q32-33, TLR-4 consists of 879 amino acids, and the extracellular domain is made up of the sequence rich in leucines. [15] TLR-4 shares a high homology with IL-1β in the inflammatory reaction.…”

Section: Discussionmentioning

confidence: 99%

Expression changes and clinical significance of TLR-4 and IL-1β in serum and knee joint fluid of patients with knee osteoarthritis

Mao¹,

Zhu

Liu³

et al. 2020

DCC

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Objective: To investigate the expression changes and clinical significance of Toll-like receptor-4 (TLR-4) and interleukin-1β (IL-1β) in the serum and knee joint fluid in patients with knee osteoarthritis (KOA).Methods: From June 2017 to December 2018, 220 cases of patients with KOA who were admitted in Department of Orthopedics of the Third Affiliated Hospital of Inner Mongolia Medical University (“our hospital” for short) were selected as the observation group. According to the severity of KOA, they were divided into the mild subgroup (n = 98), the moderate subgroup (n = 80) and the severe subgroup (n = 42). In addition, 60 cases of volunteers who received the physical examination in Health Medical Center of our hospital were selected as the control group. The levels of TLR-4 and IL-1β in the serum and knee joint fluid were detected by enzyme-linked immunosorbent assay, in order to analyze the correlation of TLR-4 and IL-1β levels with the severity of KOA.Results: The levels of TLR-4 and IL-1β in the serum and knee joint fluid of the observation group were significantly higher than those of the control group, and the differences were statistically significant (all p < .05). The levels of TLR-4 and IL-1β in the serum and knee joint fluid of the severe subgroup were significantly higher than those of the mild subgroup and the moderate subgroup, and the levels of TLR-4 and IL-1β in the serum and knee joint fluid of the moderate subgroup were higher than those of the mild group. The differences were statistically significant (all p < .05). The levels of TLR-4 and IL-1β in the serum and knee joint fluid were positively correlated to the severity of KOA (r = .706, .729, .741, .715, all p < .05); in the serum, the level of TLR-4 was positively correlated to the level of IL-1 (r = .720, p < .05); in the joint fluid, the level of TLR-4 was positively correlated to the level of IL-1 (r = .736, p < .05).Conclusions: The levels of TLR-4 and IL-1β in the serum and knee joint fluid of KOA patients are abnormally increased, which is closely related with the severity of KOA. It can be used for clinical prediction, diagnosis and treatment of KOA.

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Immunohistochemical expression of TLR-4 in temporomandibular joint dysfunction

Cited by 6 publications

References 21 publications

NFkB and MMP-13 expression in condylar cartilage of temporomandibular joint with occlusal disharmony in vivo

NFkB and MMP-13 expression in condylar cartilage of temporomandibular joint with occlusal disharmony in vivo

Innate Immunity in the Development of Connective Tissue Dysplasia: Pilot Study in Children with Congenital Hip Dislocation

Expression changes and clinical significance of TLR-4 and IL-1β in serum and knee joint fluid of patients with knee osteoarthritis

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