Immunohistochemical Expression of the CD44 Variant 6 in Colorectal Adenocarcinoma

Ishida, Takahiro

doi:10.1007/pl00010042

Cited by 31 publications

(17 citation statements)

References 22 publications

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“…This hypothesis is supported not only by our results concerning local recurrences and the survival, but also by the data presented by Soukka et al where they showed with squamocellular carcinomas that diminished CD44v6-expression was associated with poorly differentiated rapidly growing neoplasms (Soukka et al, 1997). In a very recent report by Ishida, a decreased CD44v6 expression was associated with a higher probability of invasion but not of metastasis formation in colorectal adenocarcinomas further supporting our finding (Ishida, 2000). Recently, Kahara et al published a small descriptive study on CD44 expression in 47 STS.…”

Section: Discussionsupporting

confidence: 89%

The adhesion molecule CD44v6 is associated with a high risk for local recurrence in adult soft tissue sarcomas

et al. 2001

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Summary In many malignant diseases the expression levels of CD44 and its splice variant v6 (CD44v6) have been associated with the prognosis. The purpose of this study was to investigate the clinical significance of CD44 in adult soft tissue sarcomas (STS). 133 STS patients with a limb or superficial trunk tumour treated at the Helsinki University Central Hospital in 1987-1993 with a median follow-up time of 68 months were included in this study. The expression of CD44 and CD44v6 was determined immunohistochemically on paraffin-embedded tumour samples. 95% of the tumours expressed CD44 and CD44v6 was detected in 57%. Strong CD44 expression was associated with low grade (P = 0.04) and small tumour size (P = 0.02). In diploid tumours the CD44 expression was correlated with low S-phase fraction (P = 0.001). High expression of both, CD44 in general as well as that of CD44v6, predicted a higher risk for local recurrence (CD44: P = 0.01 and CD44v6: P = 0.05). Low CD44v6 content of the primary tumour correlated with poor survival (P = 0.02). Determining the expression of CD44 or CD44v6 in a primary STS could be a valuable tool for selecting the group of patients who might benefit from intensified local tumour treatment.

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Section: Discussionsupporting

confidence: 89%

The adhesion molecule CD44v6 is associated with a high risk for local recurrence in adult soft tissue sarcomas

et al. 2001

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“…CD44v6 was expressed in 62 (43%) of 145 tissue samples of patients with colorectal adenocarcinomas, which is within the range (42-57%) described in the literature [6,7]. In cell culture, one of three colorectal adenocarcinoma cell lines expressed CD44v6.…”

Section: Discussionmentioning

confidence: 74%

“…One isoform, CD44v6, became of special interest after a study by Gunthert et al [5], who showed that overexpression of CD44v6 confers metastatic capacity to pancreatic carcinoma cell line. High expression levels of CD44v6 have been described in 42% and 57% of colorectal carcinomas [6,7]. However, the prognostic relevance of CD44v6 in colorectal cancer has been considered controversial.…”

Section: Introductionmentioning

confidence: 98%

Epithelial hyaluronic acid and CD44v6 are mutually involved in invasion of colorectal adenocarcinomas and linked to patient prognosis

et al. 2004

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Desmoplastic stroma of colorectal adenocarcinomas contains a variety of extracellular matrix molecules, including hyaluronic acid (HA). Overexpression of the HA receptor CD44 and, in particular, its splicing variant CD44v6 has been described as a prognostic factor for patients with colorectal adenocarcinomas in some studies, but converse reports also exist. Our hypothesis is that these divergent results may be related to the fact that the function of CD44v6 depends on the HA content of cell-surrounding matrix. Therefore, we studied the expression of HA and CD44v6 in tissue samples of 145 patients suffering from colorectal adenocarcinomas using immunohistochemistry. Expression of HA was separately evaluated in tumor epithelium and stroma. We additionally examined the influence of HA on invasion and adhesion of colorectal adenocarcinoma cells in vitro. The results show that epithelial HA expression was not correlated with tumor stage but with lymph-node or distant metastasis. Patients with tumors expressing epithelial HA had a decreased overall survival ( P=0.017) as well as tumors with coexpression of epithelial HA and CD44v6 ( P=0.011). The latter issue remained an independent prognostic factor in multivariate analysis (relative risk 5.06; 95% confidence interval, 1.18-21.57; P=0.028). HA exclusively stimulated in vitro invasion of CD44v6-expressing cells. This stimulation was partly reversed by an anti-CD44v6 antibody. Our findings suggest that the adverse prognostic effect of CD44v6 in colorectal adenocarcinoma might be restricted to those tumors that have pericellular HA.

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“…This appears to be contrary to other reports that demonstrate a correlation between CD44v6 expression in gallbladder carcinoma and extent of histological differentiation (Yanagisawa et al 2001a). However, in the case of colorectal adenocarcinoma there is some disagreement regarding the clinical significance of CD44v6 expression (Ishida 2000). Several authors report an association of CD44v6 expression with advanced stage and poor prognosis in colorectal carcinoma (Mulder et al 1997), whereas other investigators failed to find a correlation between expression and progression of colorectal carcinoma (Ishida 2000).…”

Section: Discussionmentioning

confidence: 99%

An Immunohistochemical Study of the Expression of Adhesion Molecules in Gallbladder Lesions

Choi

Xuan

Shin

et al. 2004

J Histochem Cytochem.

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S U M M A R YWe investigated the expression of 10 adhesion molecules ( ␣ -catenin, ␤ -catenin, ␥ -catenin, CD44, CD44v6, ICAM-1, CD56, CEA, E-cadherin, and CD99) in 46 gallbladder carcinomas, 14 adenomas, 15 low-grade dysplasias, nine intestinal metaplasias, and 20 samples of normal gallbladder epithelium by immunohistochemistry. The expression of adhesion molecules was altered in gallbladder carcinomas and adenomas. In gallbladder carcinomas, increased expression of ICAM-1, CEA, and CD44v6 was observed, together with decreased expression of ␣ / ␤ / ␥ -catenin and CD99. In adenomas, aberrant expression of CD44v6 and CD56, as well as reduced expression of ␣ / ␤ / ␥ -and E-cadherins, was noted. Expression of ␣ / ␤ / ␥ -catenin was reduced in low-grade dysplasia, whereas there was no change in the expression of these adhesion molecules in metaplasia. Expression of ICAM-1, CD99, E-cadherin, and CD56 was correlated with clinical stage. In addition a correlation was noted between expression of ICAM-1 and E-cadherin and lymph node metastasis ( p Ͻ 0.05). These results suggest that altered expression of these adhesion molecules is involved in the progression and metastasis of gallbladder carcinomas.

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Immunohistochemical Expression of the CD44 Variant 6 in Colorectal Adenocarcinoma

Cited by 31 publications

References 22 publications

The adhesion molecule CD44v6 is associated with a high risk for local recurrence in adult soft tissue sarcomas

The adhesion molecule CD44v6 is associated with a high risk for local recurrence in adult soft tissue sarcomas

Epithelial hyaluronic acid and CD44v6 are mutually involved in invasion of colorectal adenocarcinomas and linked to patient prognosis

An Immunohistochemical Study of the Expression of Adhesion Molecules in Gallbladder Lesions

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