Immunohistochemical expression of TFIIH and XPF in oral tongue squamous cell carcinoma

Sá, Melka Coelho; Santana, Thalita; Santos, Edilmar de Moura; Morais, Everton Freitas de; Galvão, Hébel Cavalcanti; Freitas, Roseana de Almeida

doi:10.1007/s00405-019-05757-2

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…16 XPF partners with ERCC1 to form a bi-partite nuclease that is essential for nucleotide excision repair (NER). 17,18 XPF-ERCC1 heterodimer is directed to the damaged strand by RPA (replication protein A) to create an incision 5' to the lesion and remove the single-stranded nucleotide through XPG activation. 18,19 Studies indicate that the expression of these proteins is deregulated in various neoplastic processes, including SCC; however, the role of these proteins in the biological behavior of OTSCC and LLSCC remains controversial.…”

Section: Introductionmentioning

confidence: 99%

“…17,18 XPF-ERCC1 heterodimer is directed to the damaged strand by RPA (replication protein A) to create an incision 5' to the lesion and remove the single-stranded nucleotide through XPG activation. 18,19 Studies indicate that the expression of these proteins is deregulated in various neoplastic processes, including SCC; however, the role of these proteins in the biological behavior of OTSCC and LLSCC remains controversial. 3,20 In this context, studies analyzing the role of the DNA repair proteins XRCC1 and XPF in OTSCC are scarce and, so far, no study has evaluated their immunoexpression in LLSCC.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical expression of DNA repair proteins in oral tongue and lower lip squamous cell carcinoma

et al. 2020

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The DNA repair system involves genes and proteins that are essential for the maintenance of genome integrity and the consequent control of various cellular processes. Alterations in these genes and proteins play a role in tumor development and progression and might be associated with prognosis. The aims of this study were to analyze the immunoexpression of two DNA repair proteins, XPF and XRCC1, in lower lip squamous cell carcinoma (LLSCC) and oral tongue squamous cell carcinoma (OTSCC), and to investigate possible associations with clinical and histopathological parameters. The immunohistochemical expression of XPF and XRCC1 was analyzed semi-quantitatively in 40 cases each of LLSCC and OTSCC. The chi-squared test or Fisher's exact test, when appropriate, was used to investigate the association between expression of the proteins and clinicopathological characteristics. The cytoplasmic immunoexpression of XPF was high in OTSCC (95% of the cases analyzed) but low in LLSCC (52.5%). Among the clinicopathological parameters evaluated, a statistically significant association was observed between high nuclear expression of XRCC1 and the absence of regional lymph node metastasis in patients diagnosed with OTSCC (p=0.006). The high protein expression of XPF and XRCC1 in OTSCC and LLSCC suggests an important role in the development and progression of these tumors. Our study found an association between high nuclear expression of XRCC1 and the absence of loco-regional metastasis in cases diagnosed as OTSCC, suggesting a role of this protein in tumor progression.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical expression of DNA repair proteins in oral tongue and lower lip squamous cell carcinoma

et al. 2020

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“…This subtype counts for about 90% of oral cancers. Patients with this malignant neoplasm show high morbidity and poor prognosis, with 5-year survival of approximately 50% (Jensen et al, 2015;Bjerkli et al, 2020;Dourado et al, 2020;Sá et al, 2020). Although the oral cavity allows easy inspection, 60% of OSCCs are diagnosed on an advanced stage.…”

Section: Introductionmentioning

confidence: 99%

Tumor Budding and Poor Prognosis in Oral Cancer: A Systematic Review and Meta-Analysis

Feitosa,

Oliveira,

Bezerra

et al. 2023

Asian Pac J Cancer Prev

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Background: Tumor budding (TB) has been investigated in several types of solid tumors. In oral cancer, studies show its association with survival. However, for its implementation in routine histological analyses, results with a high certainty of evidence are needed. Therefore, the aim of this systematic review is to explore the association between tumor budding and overall survival (OS), disease-specific survival (DSS), and disease-free survival (DFS) in oral cancer. Methods: A search was performed in Embase, PubMed, Scopus, Livivo, Web of Science, and Google Scholar. We adopted the following inclusion criteria: studies that evaluate tumor budding in oral cancer, that investigate survival, and presenting cohort design. We excluded reviews and studies without hazard-ratio (HR) data. Results: This systematic review included 22 studies and showed an association between TB and survival. High-grade TB is associated with a worse OS in univariate analysis (HR = 3.11; 95% CI: 2.06-4.69, p<0.01) and multivariate analysis (HR = 2.62; 95% CI: 1.64-4.20, p<0.01); with a poorer DSS in univariate (HR = 2.43; 95% CI: 1.94-3.03, p<0.01) and multivariate analysis (HR = 2.01; 95% CI: 1.43-2.83, p< 0.01); and with a worse DFS in univariate (HR = 1.94; 95% CI: 1.44-2.62, p<0.01) and multivariate analysis (HR = 2.15; 95% CI: 1.31-3.53, p< 0.01). Sensitivity analysis showed that the results are robust, and no significant publication bias was identified in univariate analysis for DFS (Egger’s test: p = 0.94). The certainty of the evidence was graded as low or very low. Conclusion: Our findings indicate that TB is an independent prognostic factor of OS, DSS, and DFS in oral cancer. However, further studies are needed to increase the certainty of the evidence.

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“…Many researches have mentioned the role of ERCC4 in cancers, such as bladder cancer, gastric cancer and oral cancer ( Li & Ma, 2018 ; Qiu et al, 2014 ; Sa et al, 2020 ; Wang et al, 2010 ; Wei et al, 2014 ). Colorectal cancer (CRC) is a malignant tumor which is the third cause of cancer death in China ( Zhang et al, 2021 ).…”

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confidence: 99%

Comprehensive analysis of ceRNA network of ERCC4 in colorectal cancer

Liu

Chu

et al. 2021

PeerJ

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Objective ERCC4 is one of the most significant molecules of Nucleotide Excision Repair (NER), which has been researched due to its high expression in colorectal cancer (CRC). This study aimed to find out the ceRNA (competitive endogenous RNA) network of ERCC4 in CRC. Methods and Materials Pan cancer mRNA expression of ERCC4 was evaluated using TCGA database. The protein expression of ERCC4 was evaluated based on the Human Protein Atlas (HPA). We screened DElncRNAs and DEmiRNAs in two groups of ERCC4high and ERCC4low expression in CRC. Then a lncRNA-miRNA-ERCC4 regulatory network was constructed based on DElncRNAs and DEmiRNAs using Starbase database and visualized by Cytoscape software. Kaplan-Meier analysis was performed to evaluate the prognostic value of the ceRNA network. Further, RT-PCR was performed to validate the expression of the representative molecules in the ceRNA network in CRC and normal tissues. The relationship between drug sensitivity and these molecules were also evaluated using RNAactDrug database. Results ERCC4 was overexpressed in a variety of tumors at mRNA levels, including CRC. High expression of ERCC4 was also observed on protein level in CRC. A total of 1,885 DElncRNAs and 68 DEmiRNAs were identified from CRC samples in ERCC4high and ERCC4low expression groups. Predicted by the Starbase database, we got interacting miRNAs and lncRNAs of ERCC4 from the DEmiRNAs and DElncRNAs, and a lncRNA-miRNA-ERCC4 regulatory network was constructed. Kaplan-Meier survival curves results showed that miR-200c-3p (hazard ratio [HR] = 0.62, P = 0.032), MALAT1 (HR = 1.54, P = 0.016), and AC005520.2 (hazard ratio [HR] = 1.75, P = 0.002) were significantly associated with the prognosis of CRC. After validation by RT-PCR, we found that ERCC4 and MALAT1 were up-regulated in CRC compared with normal tissues, while miR-200c-3p was down-regulated. A strong negative correlation was observed between MALAT1 and miR-200c-3p. Drug sensitivity analysis showed that ERCC4, miR-200c and MALAT1 were all associated with Cisplatin. Conclusion We constructed a ceRNA network of ERCC4 in CRC, of which the MALAT1-miR-200c-3p-ERCC4 axis may be involved in the development, prognosis and chemotherapy sensitivity of CRC. These findings might provide novel clues and insights on the molecular mechanisms of ERCC4 and NER pathway in CRC.

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Immunohistochemical expression of TFIIH and XPF in oral tongue squamous cell carcinoma

Cited by 5 publications

References 30 publications

Immunohistochemical expression of DNA repair proteins in oral tongue and lower lip squamous cell carcinoma

Immunohistochemical expression of DNA repair proteins in oral tongue and lower lip squamous cell carcinoma

Tumor Budding and Poor Prognosis in Oral Cancer: A Systematic Review and Meta-Analysis

Comprehensive analysis of ceRNA network of ERCC4 in colorectal cancer

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