Immunohistochemical Expression of P16 in Low Grade Urothelial Carcinoma

Chaudry, Muhammad Umer; Akhtar, Farhan; Jamal, Nighat; Mansur, Hamza; Sarwar, Nozaif; Saleem, Yusra

doi:10.51253/pafmj.v71i3.6075

Cited by 1 publication

(2 citation statements)

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“…Urothelial carcinoma of the urinary bladder is one of the most common malignancies in the world, and the prevalence varies across different countries and may differ between the regions of the same country [ 18 , 19 ]. A higher prevalence of urothelial carcinoma of the urinary bladder has been documented in Egypt [ 3 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we found a correlation between p16 protein expression and the histological grade of papillary urothelial carcinoma, in addition to a correlation between p16 expression and the muscle invasion of papillary urothelial carcinoma. Previous studies stated that identification of p16 protein expression directly correlates with the tumor behavior and tumor stage where the low-grade tumors and the NMIBCs showed a high expression rate of p16 protein which may help in the process of selecting patients for an early aggressive therapy [ 13 , 18 ]. However, its prognostic significance and the diagnostic utility according to the staining intensity are still unclear, and the studies showed quite different results.…”

Section: Discussionmentioning

confidence: 99%

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Clinico-Pathological Features and Immunohistochemical Comparison of p16, p53, and Ki-67 Expression in Muscle-Invasive and Non-Muscle-Invasive Conventional Urothelial Bladder Carcinoma

Hasan,

Mohammed,

Basiony

et al. 2023

Clinics and Practice

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Introduction: The identification of bladder detrusor muscle invasion in urothelial cancer is essential for prognosis and management. We studied the clinical, histological, and immunohistochemical expression of p16, p53, and Ki-67 in urothelial detrusor muscle-invasive bladder cancer (MIBC) and urothelial non-detrusor muscle-invasive bladder cancer (NMIBC) in Egyptian patients. Methods: Sixty-two bladder urothelial cancer cases obtained through TURBT were included and divided into two groups: (MIBC, stage T2) and NMIBC (T1). Tissue blocks were recut and re-examined microscopically; then, the immunostaining of p16, p53, and Ki-67 was performed to compare both groups and evaluate the 13% cut-off for Ki-67, 20% for p53, and p16 intensity in various conditions aided by telepathology technology. Results and conclusion: Hematuria was the main clinical first presentation, with no significant difference between either group. The mean age was 61.6 years, with male predominance (52 males and 10 females). The absence of papillary histological pattern was associated with a higher stage, including detrusor muscle invasion (p = 0.000). The overall average percent of p53 immunostaining was 12.9%, revealing no significant difference between MIBC and NMIBC when a cut-off of 20% was implicated. The Ki-67 expression was correlated with higher grade and muscle invasion; however, no association was found with the other two markers’ expression. The negative immunostaining of p16 was associated with low grade and NMIBC in the case of the preservation of the papillary pattern. We recommend further studies on the cut-off of widely used markers and more immunohistochemical and genetic studies on the p16(INK4A), taking into consideration the histological pattern of conventional carcinomas.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%