Immunohistochemical expression of monoclonal antibody 43‐9F in testicular germ cell tumours

Heidenreich, Axel; Ia, Sesterhenn; Fk, Mostofi; Jw, Mason

doi:10.1046/j.1365-2605.1998.00131.x

Cited by 12 publications

(12 citation statements)

References 15 publications

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“…Interestingly, the putative nonmalignant counterpart of carcinoma in situ has been suggested to be present around the 9th to 10th week of intrauterine development (Jørgensen et al, 1995), which would be in accordance with our present findings. This is also in agreement with epidemiological data (Adami et al, 1994), immunohistochemical findings (Cummings et al, 1994;Heidenreich et al, 1998;Hittmair et al, 1996;Rajpert-De Meyts and Skakkebaek, 1994;Roelofs et al, 1999), and expression analysis of human endogenous retrovirus K (HERV-K)-specific transcripts (Herbst et al, 1999;Roelofs et al, 1998). These markers are overall negative in normal spermatogenesis and spermatocytic seminomas, and positive in TGCTs.…”

Section: Stoop Et Alsupporting

confidence: 89%

Reactivity of Germ Cell Maturation Stage-Specific Markers in Spermatocytic Seminoma: Diagnostic and Etiological Implications

Stoop

Gurp

Krijger

et al. 2001

Laboratory Investigation

100

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SUMMARY:It is generally accepted that testicular seminomas and spermatocytic seminomas have separate pathogeneses, although the origin of these two types of germ cell tumors of the adult testis remains a matter of debate. Although an embryonic germ cell origin seems to be most likely for seminomas, a spermatogonia-spermatocyte origin has been suggested for spermatocytic seminoma. To shed more light on the etiology of spermatocytic seminomas, we undertook an immunohistochemical and molecular approach using SCP1 (synaptonemal complex protein 1), SSX (synovial sarcoma on X chromosome), and XPA (xeroderma pigmentosum type A) as targets. Although a stage-specific expression pattern has been reported for SCP1 and SSX in normal spermatogenesis, we demonstrate here that it also exists for XPA. In fact, immunohistochemistry shows that the proteins of SCP1 and XPA are specifically present in the stage of primary and pachytene spermatocytes. In contrast, SSX was found in spermatogonia and primary spermatocytes, as well as in germ cells, from at least the 17th week of intrauterine development onward. Although no protein encoded by any of these genes was detected in tumor cells of a series of testicular seminomas, all tested spermatocytic seminomas were positive, in agreement with expression analysis. These data support the model that seminomas originate from an embryonic germ cell, and they imply that the cell of origin of spermatocytic seminomas is at least capable of maturing to the stage of spermatogonia-pachytene spermatocyte. (Lab Invest 2001, 81:919 -928).

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Section: Stoop Et Alsupporting

confidence: 89%

Reactivity of Germ Cell Maturation Stage-Specific Markers in Spermatocytic Seminoma: Diagnostic and Etiological Implications

Stoop

Gurp

Krijger

et al. 2001

Laboratory Investigation

100

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“…Prior studies have found a wide spectrum of positivity of AFP, from 55% to 100%, with authors noting that focal reactivity can limit the utility of the marker 18,29–33 . Furthermore, AFP has been reported to stain embryonal carcinoma; in over half of cases in some studies 30,31,33,34 . These issues have led some authors to conclude that AFP is not a specific or reliable marker for YST 29,34 …”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, AFP has been reported to stain embryonal carcinoma; in over half of cases in some studies 30,31,33,34 . These issues have led some authors to conclude that AFP is not a specific or reliable marker for YST 29,34 …”

Section: Discussionmentioning

confidence: 99%

Glypican 3 has a higher sensitivity than alpha‐fetoprotein for testicular and ovarian yolk sac tumour: immunohistochemical investigation with analysis of histological growth patterns

et al. 2010

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“…Subsequent studies identified a number of antigens that were highly expressed in CIS cells and gonocytes but not in the normal germ cell in the adult testes. Other CIS markers were identified using antibodies raised against glycolipids and glycoproteins expressed by tumour-derived cell lines, including M2A (22,(30)(31)(32), 43-9F (22,33,34) and TRA-1-60 (35). One of the first markers found was placental-like alkaline phosphatase (PLAP), a tissue-specific alkaline phosphatase with unknown biological function, which is also expressed in classical seminoma as well as primordial germ cells and early gonocytes (16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26).…”

Section: Similarities Between Cis Cells and Normal Germ Cellsmentioning

confidence: 99%

The emerging phenotype of the testicular carcinoma in situ germ cell

Meyts¹,

Bártková²,

Samson³

et al. 2003

Apmis

140

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This review summarises the existing knowledge on the phenotype of the carcinoma in situ (CIS) cell. CIS is a common pre-invasive precursor of testicular germ cell tumours of adolescents and young adults. These tumours display a variety of histological forms. Classical seminoma proliferates along the germ cell lineage, whereas embryonal carcinoma retains embryonic features and readily differentiates into teratomas that resemble various somatic cell lineages. A thorough review of the gene expression in CIS cells in comparison to normal testicular germ cells and overt tumours supports the view that CIS is a common precursor for both tumour types. Impaired cell differentiation resulting in a partial retention of the embryonic features, associated with an increasing genomic instability may be responsible for a remarkable phenotypic heterogeneity of CIS cells. Depending on the degree of differentiation and pluripotency, CIS cells found in adult patients seem to be predestined for further malignant progression into one or the other of the two main types of overt tumours. A new concept of phenotypic continuity of differentiation of germ cells along germinal lineage with a gradual loss of embryonic features based on the analysis of gene expression in all types of germ cells during their ontogeny is presented in this review. The data point out that despite the phenotypic continuum of gene expression, there are two periods of rapid changes of gene expression: first at the transition from primordial germ cells to pre-spermatogonia, and later during the pubertal switch from the mitotic to meiotic cell division. The persistent expression of embryonic traits in CIS cells, and the high expression of the cell cycle regulators that are typical of mitotic germ cells support our long-standing hypothesis that CIS cells originate from primordial germ cells or gonocytes and not from germ cells in the adult testis.

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Immunohistochemical expression of monoclonal antibody 43‐9F in testicular germ cell tumours

Cited by 12 publications

References 15 publications

Reactivity of Germ Cell Maturation Stage-Specific Markers in Spermatocytic Seminoma: Diagnostic and Etiological Implications

Reactivity of Germ Cell Maturation Stage-Specific Markers in Spermatocytic Seminoma: Diagnostic and Etiological Implications

Glypican 3 has a higher sensitivity than alpha‐fetoprotein for testicular and ovarian yolk sac tumour: immunohistochemical investigation with analysis of histological growth patterns

The emerging phenotype of the testicular carcinoma in situ germ cell

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