Immunohistochemical Expression of Ionized Calcium Binding Adapter Molecule 1 in Cutaneous Histiocytic Proliferative, Neoplastic and Inflammatory Disorders of Dogs and Cats

Pierezan, Felipe; Mansell, Joanne; Ambrus, Andy; Hoffmann, Aline Rodrigues

doi:10.1016/j.jcpa.2014.07.003

Cited by 123 publications

(114 citation statements)

References 13 publications

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“…To ascertain total macrophage abundance in healing tissue, we employed the pan-macrophage marker, ionized calcium-binding adaptor molecule 1 (IBA-1), to quantify the spatiotemporal pattern of macrophage infiltration in Acomys and Mus . IBA-1 is an actin-binding protein active in macrophages and microglia and has been used to label cells in mouse, dog, cat, human and other primates (Imai et al, 1996; Köhler, 2007; Pierezan et al, 2014; Sasaki et al, 2001; Schmidt et al, 2016). In Acomys , we found IBA-1+ cells in the mesenchyme and the epidermis, but IBA-1 was specifically absent from polymorphonuclear neutrophils (Figure 2F, arrows).…”

Section: Resultsmentioning

confidence: 99%

Macrophages are necessary for epimorphic regeneration in African spiny mice

Simkin

Gawriluk

Gensel

et al. 2017

eLife

157

162

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How the immune system affects tissue regeneration is not well understood. In this study, we used an emerging mammalian model of epimorphic regeneration, the African spiny mouse, to examine cell-based inflammation and tested the hypothesis that macrophages are necessary for regeneration. By directly comparing inflammatory cell activation in a 4 mm ear injury during regeneration (Acomys cahirinus) and scarring (Mus musculus), we found that both species exhibited an acute inflammatory response, with scarring characterized by stronger myeloperoxidase activity. In contrast, ROS production was stronger and more persistent during regeneration. By depleting macrophages during injury, we demonstrate a functional requirement for these cells to stimulate regeneration. Importantly, the spatial distribution of activated macrophage subtypes was unique during regeneration with pro-inflammatory macrophages failing to infiltrate the regeneration blastema. Together, our results demonstrate an essential role for inflammatory cells to regulate a regenerative response.DOI: http://dx.doi.org/10.7554/eLife.24623.001

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Section: Resultsmentioning

confidence: 99%

Macrophages are necessary for epimorphic regeneration in African spiny mice

Simkin

Gawriluk

Gensel

et al. 2017

eLife

157

162

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“…In contrast to rodents, Iba1 expression has not been described in the intestine of dogs until now, although it has been proven as a useful macrophage marker in the canine skin. 39 Interestingly, PAS þ macrophages in HUC revealed both nuclear, as well as cytoplasmicmembranous, staining patterns. Iba1 is a key molecule in membrane ruffling and phagocytosis and therefore functions in activated macrophages and microglia.…”

Section: Discussionmentioning

confidence: 97%

Heterogeneity of macrophages in canine histiocytic ulcerative colitis

Nolte¹,

Junginger²,

Baum

et al. 2017

Innate Immun

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Histiocytic ulcerative colitis (HUC) is a chronic enteropathy which most notably occurs in Boxer dogs and French bulldogs. The inflamed mucosa is hallmarked by large, foamy, periodic acid-Schiff (PAS)-positive macrophages infiltrating the colonic mucosa. As little is known about their origin and phenotype, an immunohistochemical study was performed using different macrophage markers. Generally, canine colonic macrophages showed high expression of ionised calciumbinding adaptor molecule 1 and MHC class II. In canine HUC, macrophages revealed up-regulation of lysozyme and L1 Ag but decreased CD163 expression compared with controls, suggesting them to be pro-inflammatory cells, whereas the healthy colonic mucosa was characterised by an anti-inflammatory macrophage phenotype. In addition, PAS reaction was used to discriminate macrophage subpopulations. PAS -macrophages displayed higher expression of L1 Ag and CD64, whereas PAS þ cells, which were only present in HUC patients, were characterised by increased expression of lysozyme, inducible nitric oxide synthase and CD204. This indicates PAS þ cells to be mature macrophages contributing to the inflammatory process, which are most likely maintained by differentiation of immature PAS -macrophages continuously recruited from blood monocytes. In summary, macrophage heterogeneity in canine HUC probably illustrates their different maturation states and functions compared with the healthy animals.

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“…All cultured cells (FB‐LCH01, PWC‐HS01 and FCR‐HS02) were positive for histiocytic markers (HLA‐DR, CD1a, Iba‐1), S100 and vimentin (Table ) . FB‐LCH01 cells also were positive for CD11c which was known as a dendritic marker .…”

Section: Discussionmentioning

confidence: 99%

Establishment of cell line and in vivo mouse model of canine Langerhans cell histiocytosis

Son

Uchida

Thongtharb

et al. 2019

Vet Comparative Oncology

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A cell line named FB‐LCH01, derived from a dog diagnosed with Langerhans cell histiocytosis (LCH), was established and characterized. FB‐LCH01 had C‐shaped nucleoli, characterized by modal chromosome aberrations. The original tumour cells as well as established FB‐LCH01 cells were immunopositive for human leukocyte antigen‐DR, Iba‐1 and E‐cadherin, and immunonegative for CD163 and CD204, suggesting Langerhans cell origin. Furthermore, the characteristics of FB‐LCH01 were compared with those of two canine histiocytic sarcoma cell lines (PWC‐HS01 and FCR‐HS02) established previously. Expression of E‐cadherin was detected only in FB‐LCH01, but not in PWC‐HS01 and FCR‐HS02. All (n = 9) the severe combined immunodeficiency mice inoculated with the FB‐LCH01 cells developed subcutaneous tumour masses after 3 weeks. Eight of nine mice also developed metastatic lesions in the lymph nodes (8/8; 100%), lung (5/8; 62.5%), stomach (5/8; 62.5%), heart (4/8; 50%), pancreas (4/8; 50%), kidney (3/8; 37.5%), skin (3/8; 37.5%) and bone marrow (1/8; 12.5%). Tumour cells were pleomorphic and round‐ to polygonal‐shaped with prominent anisocytosis and anisokaryosis. The xenotransplanted tumour cells maintained the immunohistochemical features of the original tumour with persistent E‐cadherin expression at injection site and some visceral organs. In conclusion, the established cell line as well as the mice xenotransplant model in this study reflect the nature of canine LCH and may serve as promising models for investigating the patho‐tumorigenesis and therapy of the disease.

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Immunohistochemical Expression of Ionized Calcium Binding Adapter Molecule 1 in Cutaneous Histiocytic Proliferative, Neoplastic and Inflammatory Disorders of Dogs and Cats

Cited by 123 publications

References 13 publications

Macrophages are necessary for epimorphic regeneration in African spiny mice

Macrophages are necessary for epimorphic regeneration in African spiny mice

Heterogeneity of macrophages in canine histiocytic ulcerative colitis

Establishment of cell line and in vivo mouse model of canine Langerhans cell histiocytosis

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