Immunohistochemical Expression of Interleukin-17 and Hormonal Receptors in Benign and Malignant Breast Lesions

Abstract

Objectives: IL17 is a critical pro-inflammatory cytokine that is involved in inflammation, multidrug resistance and growth persistence pathways in cancer. This study is aiming at studying the expression of IL17 and hormonal receptors expression in benign and malignant breast lesions using immunohistochemical staining methods.Results: A total of 137 cases of breast lesions were studied, 97 (70.8%) were malignant and 40 (29.2%) were benign cases. Age range for malignant and benign cases were between 26 to 80 years [mean age 50±2 years], and 20 to 70 years [mean age 41±4 years], respectively, Odds ratio= 2.3 [1.78-1.99, 95% CI]. The majority of the histopathological diagnosis of the benign and malignant lesions were 21 (15.3%) fibro-adenomas and 87 (63.5%) invasive ductal carcinoma, respectively. Expression of IL17 and age were insignificantly negatively correlated for both groups; benign cases [r= -0.054, P value 0.742] and malignant cases [r= -0.080, P value 0.444]. IL17 expression was showing insignificant association with age group, P value 0.065. IL17 expression showed a statistical significance based on the different histopathological diagnosis, P value 0.035. Expression levels of estrogen, progesterone, and human epidermal receptors were showing insignificant difference among IL17 expression categories, P values 0.678, 0.623, and 0.361, respectively.

Comparative evaluation of host immune response and cytokine signature pertaining to Th1 and Th2 immune arms in serum and tissue among patients of acute localised vs. chronic disseminated dermatophytosis

“…Positive and negative controls were run during all reactions and to perform negative control the primary antibody was omitted. Expression of cytokine in tissue was performed semi‐quantitatively based on the overall intensity of staining on the cytoplasm by a pathologists based on percentage of cells expressing stain (10%–30% mild, 40%–60% moderate, >60% strong) 7 . Skin scrapping from advancing margin of active lesion was obtained for fungal culture by standard inoculation technique on Sabouraud dextrose agar.…”

Association of three single nucleotide polymorphisms of interleukin -35 for detection and prediction of women with breast cancer in Erbil city

“…Different polymorphisms in the promoter regions of the gene influence this shift, which can increase a person's susceptibility to cytokine-induced chronic inflammation. Proinflammatory cytokines in the tumor microenvironment have been related to a poor prognosis in breast cancer patients, according to research (Ali et al, 2020). In addition, SNPs, for example, may disrupt normal gene expression patterns and lead to inefficient immune responses, which in turn elevate cancer risks.…”

Association of interleukin-17A rs2275913 polymorphism with rheumatoid arthritis susceptibility in Sudanese population

“…19 In the IL-17 gene, there were several polymorphisms detected that might influence the expression of IL-17 . 20,21 Not only the IL-17A - 197G/A (rs2275913) was associated with the risk to RA, but also the severity of the disease and the response to treatment. 22,23 Therefore, this study aimed to investigate the association between IL-17A-197G/A (rs2275913) single nucleotide polymorphism and RA among Sudanese RA patients.…”