Immunohistochemical expression of HER ‐ 2 and Ki ‐ 67 in granulosa cell tumor in bitches

Matos, Andréa Cardoso de; Consalter, Angélica; Batista, Barbara P.; Fonseca, Ana Beatriz Monteiro; Ferreira, Ana Maria Reis; Leite, Juliana da Silva

doi:10.1111/rda.13903

Cited by 5 publications

(4 citation statements)

References 28 publications

(35 reference statements)

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“…As expected, the proliferation rate was low in our GCT, supporting the benignity of these types of tumors. In a previous study, the mean Ki-67 index was 1.69 ± 3.00% in benign human ovarian tumors (with granulosa cell tumors among them) compared with 38.75 ± 23.61% in malignant tumors [ 1 ], and in another, it was 3.2 ± 3.7% compared with 33.1 ± 16.7% [ 15 ], which is in accord with bovine [ 44 ] and canine [ 17 ] GCTs as well as with our results.…”

supporting

confidence: 58%

Immunohistochemical markers for equine granulosa cell tumors: a pilot study

DOLIN,

SCHWEIGER,

WASELAU

et al. 2023

JES

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Sex cord–stromal tumors (SCSTs), generally referred to as granulosa cell tumors (GCTs) or granulosa-theca cell tumors (GTCTs) in equids, show complex compositions and variable numbers of hormone-producing cells. These tumors can be difficult to diagnose, especially in early stages. Therefore, we tested a panel of antibodies for vimentin, smooth muscle actin, laminin, Ki-67, E-cadherin, calretinin, moesin, p-ezrin, AMH, and aromatase, markers used for tumor composition and classification, progression, and prognosis in human SCSTs, on an exemplary grapefruit-size equine GCT within the left ovary of a 13-year-old mare with stallion-like behavior and elevated testosterone levels in comparison with normal ovarian tissue. The tumor showed a low proliferation rate and prominent moesin and p-ezrin staining in granulosa cells. E-cadherin, calretinin, aromatase, and AMH are suggested to be potential markers for different cell components of equine SCSTs that can support tumor diagnosis and classification.

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supporting

confidence: 58%

Immunohistochemical markers for equine granulosa cell tumors: a pilot study

DOLIN,

SCHWEIGER,

WASELAU

et al. 2023

JES

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“…At the end, the nuclei were counterstained with hematoxylin and mounted with DAB. Interpretation criteria [14,15] tumor cell nuclei with brownish-yellow granules accounted for ≥10% were recorded as a positive expression of P receptor and E receptor; positive staining cells accounted for ≥14% were recorded as high expression of Ki67 antigen, otherwise, it was recorded as low expression of Ki67 antigen; cell membrane molecules stained at a different level were recorded as 0, +, ++ and +++, among which 0 and<puncsp> </puncsp>+<puncsp> </puncsp>are recorded as HER factor 2 negative, +++ is recorded as HER factor 2 positive, ++ requires, respectively further in situ hybridization gene amplification verification. 3 shows that the AUC of the UES is used for evaluating the expression of P receptor, HER factor 2 and Ki67 were 0.704 (95% CI:0.600-0.795), 0.763 (95% CI:0.663-0.845) and 0.820 (95% CI:0.726-0.893), respectively, as shown in Table 3 and Figure 2.…”

Section: Detection Of Biological Prognostic Factorsmentioning

confidence: 99%

[Retracted] Correlation Analysis of Ultrasound Elastography Score with Invasive Breast Cancer and Biological Prognostic Factors

Wan

Qin

et al. 2022

Contrast Media & Molecular Imaging

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Invasive breast cancer (IBC) is a kind of malignant tumor in which cancer cells have broken through the basement membrane of breast ducts or lobular acini and invaded the stroma. Although ultrasound elastography score (UES) has shown unique advantages in the diagnosis of IBC, its value in the prognosis is not clear. Here, we explored the correlation of UES with IBC and biological prognostic factors. The datum of 86 patients with suspected IBC from January 2018 to December 2021 was collected. UE was applied in the examination of all patients. The lesion tissue of the malignant group was punctured to detect and analyze the expression of biological prognostic factors, including estrogen receptor (E receptor), progesterone receptor (P receptor), and human epidermal growth factor 2 (HER factor 2) and Ki67. The differences in UES under different biological prognostic factors were compared. The receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of UES of IBC and the expression of biological prognostic factors. Based on the pathological diagnosis results, the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of UES in the diagnosis of IBC were analyzed. The correlation of UES with IBC and biological prognostic factors was analyzed by multiple linear regression and Spearman method. ROC analysis showed that the area under the curve of UES for diagnosing IBC and evaluating the expression of P receptor, HER factor 2, and Ki67 were 0.877, 0.704, 0.763, and 0.820, respectively (P＜0.05). The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of UES when diagnosing IBC were 92.42%, 90.00%, 91.86%, 96.83%, and 78.26%, respectively. The UES of E receptor expression (positive and negative group) showed no obvious variance ( P > 0.05). The UES of P receptors (positive and negative), HER factor 2 (positive and negative), and Ki67 (high and low expression) showed obvious differences ( P < 0.05). Multiple linear regression and Spearman indicated UES was significantly correlated with the expression of P receptor, HER factor 2, and Ki67 (P＜0.05). UES has a certain diagnostic value for IBC and is significantly correlated to the expression of P receptor, HER factor 2, and Ki67, which is helpful for evaluating the prognosis of patients with IBC.

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“…The veterinary literature on ErbB family gene expression is extensive. The expression of epidermal growth factor receptors has been reported in several cancer types, including canine mammary carcinoma [25], gastric epithelial tumors [36], urothelial neoplasms [37,38], ovarian tumors [39], osteosarcoma [40], lung cancer [41], anal sac gland tumors [42] and feline mammary tumors [43,44], endometrial tumors [45], and pulmonary carcinoma [46]. Despite melanoma being among the first tumors in which EGFR is suggested to be a metastatic marker [47], little is known about ErbB receptors in this cancer, and the literature about this issue is sometimes controversial.…”

Section: Discussionmentioning

confidence: 99%

A Preliminary Evaluation of the Prognostic Role of HER-2 and HER-3 Immunohistochemical Expression in Canine Melanomas

Parisi,

Aurisicchio,

Pecorari

et al. 2024

Animals

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Canine melanoma is a malignant and aggressive neoplasm showing clinical, histological, and molecular features similar to the human counterpart. In human medicine, epidermal growth factor receptors (EGFRs) have already been suggested as prognostic markers and potential therapeutic targets in cutaneous melanoma. The aim of this study was to evaluate the expression of HER-2 and HER-3 in canine melanomas by immunohistochemistry and correlate their expression to the clinicopathological parameters of the examined tumors. Thirty-seven canine melanoma samples were recruited. Data regarding signalment and clinical parameters were also collected. The population was composed of 18 cutaneous, 16 oral/mucosal, and three digital/foot pad melanomas. Histopathological investigations were carried out to analyze histological type, ulceration, and mitotic count. On each sample, immunohistochemistry was performed using an anti-Melan-A or anti-Melanoma antigen, i.e., anti-HER-2 and anti-HER-3 antibodies. HER-2 and HER-3 positivity were classified using already established scoring criteria and a statistical analysis was carried out. The results highlighted that HER-2 expression was observed in 48.6% of the samples and HER-3 expression in 18.9%. The highest HER 2 score (3+) was recorded in 16.2% of the samples, while the coexpression of the two receptors was detected in 13.5% of the samples. A statistically significant association (p < 0.05) was observed between the expression of HER-2 and HER-3 and the presence of ulceration in oromucosal tumors. This work confirms the expression of HER-2 and HER-3 in canine melanomas and suggests a putative association with negative prognostic parameters. Further studies are necessary to strengthen these data by increasing the samples size and combining pathological examinations with molecular biology in the investigation of EGFR family receptors.

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Immunohistochemical expression of HER ‐ 2 and Ki ‐ 67 in granulosa cell tumor in bitches

Cited by 5 publications

References 28 publications

Immunohistochemical markers for equine granulosa cell tumors: a pilot study

Immunohistochemical markers for equine granulosa cell tumors: a pilot study

[Retracted] Correlation Analysis of Ultrasound Elastography Score with Invasive Breast Cancer and Biological Prognostic Factors

A Preliminary Evaluation of the Prognostic Role of HER-2 and HER-3 Immunohistochemical Expression in Canine Melanomas

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