Immunohistochemical Expression of Growth Hormone Secretagogue Receptor (GSH-R) of Adipose Tissue Macrophagesin Obese Bariatric Patients

Timofte, Daniel; Mocanu, Veronica; Eloae, Florin Zugun; Hristov, Ioana; Cretu, Iustina Silivestru; Aursulesei, Viviana; Bălan, Gheorghe G.; Ciuntu, Bogdan Mihnea; Oboroceanu, Teodor; Tiron, Adrian; Costan, Victor Vlad; Butcovan, Doina

doi:10.37358/rc.19.9.7563

Cited by 4 publications

(5 citation statements)

References 21 publications

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“…There is little information about the immunohistochemical expression of GSH-R in human adipose tissues (8). Our current data showed positive immunohistochemical expression of GSH-R associated with CD68-macrophage infiltration in epicardial adipose tissue.…”

Section: Discussionsupporting

confidence: 41%

“…In AT, ghrelin through its receptor GHS-R1a may promote adipose tissue inflammation, increased macrophage infiltration, and promoted macrophage polarization to M1 (7). In a previous paper, we showed that adipocyte diameter, ATM accumulation, and immunohistochemical expression of the ghrelin receptor in AT are associated with obesity (8). Ghrelin through its growth hormone secretagogue receptor (GHS-R1a), present on ATMs, may have a pro-inflammatory effect, and deletion of GHS-R1a would decrease the macrophage infiltration in adipose tissue (7,8).…”

Section: Introductionmentioning

confidence: 84%

“…In a previous paper, we showed that adipocyte diameter, ATM accumulation, and immunohistochemical expression of the ghrelin receptor in AT are associated with obesity (8). Ghrelin through its growth hormone secretagogue receptor (GHS-R1a), present on ATMs, may have a pro-inflammatory effect, and deletion of GHS-R1a would decrease the macrophage infiltration in adipose tissue (7,8). The effect of ghrelin and its receptors on pericardial adiposity is less known and could provide new therapeutic opportunities for ghrelin in cardiovascular medicine.…”

Section: Introductionmentioning

confidence: 95%

“…IHC procedures were used (8) for assessing the GSH-R (GHSR Polyclonal Antibody, ThermoFisher Scientific, PA5-28752) and CD68 (Dako, IS613) expression of the macrophages in periatrial adipose tissue samples from obese POAF patients.…”

Section: Immunohistochemistry Of CD 68 and Ghs-r In Periatrial Eatmentioning

confidence: 99%

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Association of Ghrelin Receptor and Inflammation in Peri-Atrial Adipose Tissue from Obese Patients with Postoperative Atrial Fibrillation

et al. 2020

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Context. Atrial fibrillation (AF) is the most common sustained arrhythmia in clinical practice. The increasing evidence supports links between inflammation and AF. There is evidence showing that obesity is a major cause of adipose tissue (AT) inflammation. Ghrelin (GHRL), through its growth hormone secretagogue receptor (GHS-R) present on adipose tissue macrophages (ATMs), could modulate AT inflammation.Objective. Our study aimed to evaluate the role of adipose tissue macrophages (ATMs) and their GHS-R in adipose tissue samples of right atrial appendages (RAA) biopsies.Subjects and Method. We obtained RAA biopsies from 10 obese patients, undergoing cardiac surgery for coronary artery bypass graft (CABG) and developing postoperative atrial fibrillation (POAF). The epicardial tissue samples were examined using immunohistochemistry to visualize and quantify CD68 and GSH-R expression of the ATMs.Results. Histologically, the mean adipocyte diameter (MAD) of epicardial adipose tissue (EAT) was larger in EAT samples with inflammation as compared to EAT without inflammation (84.2 µm vs. 79.6 µm). The expression of CD68 was lower in EAT without inflammation as compared to EAT with inflammation in adipose tissue samples. Similarly, the expression of GSH-R was lower in EAT samples without inflammation as compared to EAT samples with inflammation in adipose tissue.Conclusions. Increased epicardial fat area, macrophage infiltration, and GHS-R expression in epicardial ATMs appeared to be associated with postoperative atrial fibrillation in obese patients.

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Section: Discussionsupporting

confidence: 41%

Section: Introductionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 95%

Section: Immunohistochemistry Of CD 68 and Ghs-r In Periatrial Eatmentioning

confidence: 99%

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Association of Ghrelin Receptor and Inflammation in Peri-Atrial Adipose Tissue from Obese Patients with Postoperative Atrial Fibrillation

et al. 2020

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“…Ghrelin through its receptor GHS-R1a may promote adipose tissue inflammation, increase macrophage infiltration and promote macrophage polarization to M1 and increase of adipocyte diameter, immunohistochemical expression of the ghrelin receptors in atrial adipose tissue is associated with obesity [25].However, deletion of GHS-R1a would decrease the macrophage infiltration in adipose tissue [26]. Different studies have illustrated the relationship of ghrelin and obesity.It seems that ethnicity has a role in the level of ghrelin, as it was found that ghrelin concentration in obese Caucasians was significantly lower compared to those in their normal weight counterpart compared to Pima Indians [27].While another study explained ghrelin relation with the degree of insulin resistance or diabetes mellitus between obese individuals.…”

Section: Resultsmentioning

confidence: 99%

Detection of Leptin and Ghrelin Hormones and the Expression of their Receptors in Iraqi Obese Individuals

Alsaffar,

Jumaa,

Baqer

2024

Iraqi Journal of Science

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Obesity is a complex disease and a major worldwide health hazard with adult mortality. Obesity is defined by an increase in the body-mass index of 30 kg m−2 or greater. It belongs to the genetic predisposition and more consumption of high-energy foods and decreased requirement for physical activity in modern society. This study was designed to evaluate leptin and ghrelin hormones levels and the gene expression of leptin and ghrelin receptors in obese individuals. Seventy-five obese (45 females and 30 males) and 25 (15 females and 10 male) normal individuals were admitted to the Obesity Research and Therapeutic Unit at Alkindy College of Medicine/ University of Baghdad. All blood samples were pulled from obese and normal weight individuals. Leptin and ghrelin hormones were evaluated by ELISA technique and leptin and ghrelin receptors expressions were estimated by RT-PCR. Leptin level was lower in obese than normal 2.74±0.14 ng/ml, 3.47± 0.38 ng/ml respectively. While ghrelin level was higher in obese than normal (26.3 ± 0.56 ng/ml, 17.3 ± 0.5 ng/ml respectively). There was a significant decrease in leptin receptor Ob-Rb mRNA expression in obese individuals (P<0.05). Obese individuals had more ghrelin receptor GHS-R mRNA expression than normal people. In conclusion, obese patients were found to have higher ghrelin hormone and GHS-R as well as low leptin and Ob-Rb mRNA expressions.

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Macrophage Accumulation and Angiogenesis in Epicardial Adipose Tissue in Cardiac Patients with or without Chronic Heart Failure

et al. 2020

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Routinely measuring epicardial fat had become a novel tool for cardiovascular risk stratification. Structural changes in epicardial adipose tissue (EAT), including fat thickness, inflammation, and angiogenesis, have been described in coronary artery disease (CAD) patients. We proposed to measure EAT thickness and characterize inflammatory infiltrate and angiogenesis in epicardial adipose tissue in CAD patients with and without chronic heart failure (CHF), established by cardiac dysfunction on echocardiography (left ventricular ejection fraction, LVEF ≤ 50%) and symptoms of heart failure (New York Heart Association (NYHA) functional class II or III).The study included 15 patients with CAD (demonstrated by coronary angiography),, who underwent right atrial appendages (RAA) excision during coronary artery bypass graft (CABG). The study was performed by histopathological, immunohistochemical (IHC), and morphometrical analysis. EAT thickness was assessed by using morphometry applied on routine histological stains. Inflammatory cell infiltration and angiogenesis were investigated immunohistochemically by using antibodies against CD68 and CD34 markers. Diminished EAT thickness in the CAD patients with CHF was associated with increased macrophage infiltration and reduced angiogenesis of the EAT as compared to CAD patients without CHF. In conclusion, the present study on epicardial fat samples of the RAA suggested that high expression of CD68 appeared to be associated with severe deterioration of heart function in CAD patients who underwent myocardial revascularization consisting of CABG.

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Immunohistochemical Expression of Growth Hormone Secretagogue Receptor (GSH-R) of Adipose Tissue Macrophagesin Obese Bariatric Patients

Abstract: Obesity is a major cause of adipose tissue (AT) inflammation. Ghrelin (GHRL), through its growth hormone secretagogue receptor (GHS-R) present on adipose tissue macrophages (ATMs), could modulate AT inflammation.

Cited by 4 publications

References 21 publications

Association of Ghrelin Receptor and Inflammation in Peri-Atrial Adipose Tissue from Obese Patients with Postoperative Atrial Fibrillation

Association of Ghrelin Receptor and Inflammation in Peri-Atrial Adipose Tissue from Obese Patients with Postoperative Atrial Fibrillation

Detection of Leptin and Ghrelin Hormones and the Expression of their Receptors in Iraqi Obese Individuals

Macrophage Accumulation and Angiogenesis in Epicardial Adipose Tissue in Cardiac Patients with or without Chronic Heart Failure

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