Immunohistochemical Expression of Growth Factors in the Follicular Wall of Normal and Cystic Ovaries of Sows

Sant’Ana, Fabiano José Ferreira de; Reis, Janildo Ludolf; Blume, Guilherme Reis; Gimeno, Eduardo Juan; Rey, Florencia; Ortega, Hugo Héctor

doi:10.1111/rda.12495

Cited by 5 publications

(6 citation statements)

References 37 publications

(100 reference statements)

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“…to previous studies found in swine (Barboni et al 2000, Shimizu et al 2002, Sant'Ana et al 2015, human (Gordon et al 1996, Neulen et al 1998, rat (Carmeliet et al 1996, Ortega et al 2007, and bovine (Babitha et al 2013), we also found that HIF-1α and VEGF were expressed in swine GCs and their levels increased with follicular development in this study, suggesting that HIF-1α/ VEGF signals are closely related to follicular development, ovulation, and corpus luteum formation.…”

Section: Journal Of Molecular Endocrinologysupporting

confidence: 89%

“…In addition to promoting follicular development for angiogenesis, VEGF can also promote GCs proliferation and steroid hormone synthesis and inhibit apoptosis according to increasing evidence (Grasselli et al 2002, Greenaway et al 2004, Irusta et al 2010). Meanwhile, it has been demonstrated that VEGF is present at a high expression level in swine (Barboni et al 2000, Shimizu et al 2002, Sant'Ana et al 2015 and human (Gordon et al 1996) GCs before ovulation and increases prostaglandin synthesis leading to ovulation in human (Trau et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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GPER mediates the IL6/JAK2/STAT3 pathway involved in VEGF expression in swine ovary GCs

Xiao

Wang

Lü

et al. 2022

Journal of Molecular Endocrinology

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Vascular endothelial growth factor (VEGF) plays a pivotal role for angiogenesis in ovaries, particularly during the follicular development and ovulation. Interleukin-6 (IL-6) is one of the major pro-inflammatory factors that are involved in the angiogenesis process physiologically and pathologically. Previous studies have shown that 17β-estradiol (E2) stimulates VEGF expression by upregulating hypoxia-inducible factor 1α (HIF-1α) in many cell types and the high level of E2 causes an inflammatory-like microenvironment before ovulation. However, whether IL-6 signaling is involved in E2 regulating VEGF expression in swine granulosa cells (GCs) is still unknown. In this study, we found the estrogen membrane receptor, G-protein coupled estrogen receptor 1 (GPER) was expressed in swine GCs, and the expression level of GPER, HIF-1α and VEGF increased with follicular development. In vitro study showed that E2, ICI182780, and GPER agonist (G1) promoted the expressions of HIF-1α and VEGF in swine GCs, while GPER antagonist (G15) inhibited the stimulating effect of E2 and G1. Meanwhile, G15 inhibited the stimulating effect of E2 and G1 on IL-6 mRNA expression and secretion. Furthermore, IL-6 antibody and AG490 (JAK2/STAT3 inhibitor) attenuated G1-induced HIF-1α and VEGF expression. In conclusion, this study revealed how estrogen-induced HIF-1α and VEGF expressions in swine GCs are mediated through GPER-derived IL-6 secretion leading to JAK2/STAT3 activation.

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Section: Journal Of Molecular Endocrinologysupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

GPER mediates the IL6/JAK2/STAT3 pathway involved in VEGF expression in swine ovary GCs

Xiao

Wang

Lü

et al. 2022

Journal of Molecular Endocrinology

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“…Ovarian cysts (OCs) of clinical relevance are commonly found in domestic animals [ 16 ] in which they cause gynecopathies [ 2 18 28 ] and hyperestrogenism [ 15 ]. OCs can originate from different ovarian structures and their development, frequency, and size varies across species [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…The most common types are follicular cysts (FCs), cysts of subsurface epithelial structure (SES), cystic rete ovarii (CRO), lutein cysts (LCs), and cystic corpora lutea (CCL) [ 10 15 16 29 ]. While OCs and related gynecopathies are well characterized in sows [ 28 ] and cattle [ 2 ], the association of OC morphology based on histology and immunohistochemistry in relation to the clinical manifestation of the patient has rarely been investigated in dogs [ 4 6 ]. In particular, it should be noted, that histologically evaluated LCs in dogs have not been fully documented in recent reports [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Histological classification of canine ovarian cyst types with reference to medical history

et al. 2018

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Ovaries of 21 bitches presented with gynecopathies were surgically removed and histologically examined. Standard histological, as well as immunohistochemical, classification of 193 cystic structures resulted in the classification of 72 cysts of subsurface epithelial structures (SES), 61 follicular cysts (FCs), 38 cystic rete ovarii (CRO), 13 lutein cysts (LCs), and 9 non-classifiable cysts (NCCs). In addition to the histological classification, results were interpreted according to subject medical history, clinical examination outcome, and macroscopic observations during ovariohysterectomy. Dogs with ovarian cysts (OCs) and associated reproductive perturbations were mostly nulliparous, of large breed, and had an average of 9.5 ± 3 years. Prolonged or shortened inter-estrus intervals of past heats, however, seemed to be relatively low-risk factors for the development of OCs in dogs. Furthermore, we provide histological observations of a rarely seen canine LC including a degenerated oocyte in the central cavity.

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“…Steroidogenic enzymes can influence the ovarian steroids synthesis and play an essential role in the creation and/or course of cystic ovarian disease in sows (Kozlowska, Majewski, & Jana, 2009). Recent investigations revealed that apoptosis and cell proliferation (Sun et al, 2012) and the expression of cytoskeletal proteins (Sant'Ana, Reis, Araújo, Gimeno, & Ortega, 2015) and of growth factors (Sant'Ana, Reis, Blume, et al, 2015) were altered in swine cystic ovarian follicles. It is possible that pathogenesis of this important ovarian lesion to be related to changes in the expression of carbohydrates in follicles and in other ovarian structures, as previously confirmed in experimental cystic follicles of rats (Barbeito, Ortega, Matiller, Gimeno, & Salvetti, 2013).…”

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confidence: 99%

Lectin histochemical pattern on the normal and cystic ovaries of sows

Mendes

Pinto

Gimeno

et al. 2019

Reprod Domestic Animals

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The lectin histochemical pattern (LHP) was characterized and compared in normal and cystic ovaries of sows. Six biotinylated lectins (PNA, SBA, WGA, RCA‐1, DBA and UEA‐1) were used on tissue sections. In the normal ovaries, the reaction to UEA‐1 and SBA was mild to moderate in mesothelial and endothelial cells. RCA‐1 staining was mild to moderate in theca interna of growing follicles, corpora luteum and mesothelium. In addition, this lectin presented strong reaction in endothelial cells, granulosa cells of atretic follicles, zona pellucida of growing follicles and plasma. DBA showed strong intensity in mesothelial and endothelial cells. There was mild to moderate reactivity to WGA in granulosa cells, corpus luteum and theca interna of follicles in development, and moderate in zona pellucida, in granulosa cells of atretic follicles and mesothelium. PNA staining was mild to moderate in oocytes and in the adventitia and media of medullary arteries. Changes in the LHP of the cystic ovaries were noted; however, there were no differences in these findings between the follicular and luteinized cysts. UEA‐1 reactivity in the cystic ovaries was moderately reduced in the mesothelial and endothelial cells, whereas there was mild reduction in the DBA staining in the granulosa cells. Reaction to RCA‐1 and WGA in the cysts also was decreased in theca interna, zona pellucida and granulosa cells of atretic follicles. Furthermore, endothelium and theca interna in the cystic ovaries presented mild reduction of marcation to SBA, whereas there was decreased reactivity to PNA in the oocytes and adventitia and media layers of the medullary arteries. The results of the current study show that cysts modify the LHP in swine ovaries. These changes of glycoconjugates in many ovarian structures could modify diverse process and may be one of the reasons for decreased fertility in sows.

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Immunohistochemical Expression of Growth Factors in the Follicular Wall of Normal and Cystic Ovaries of Sows

Cited by 5 publications

References 37 publications

GPER mediates the IL6/JAK2/STAT3 pathway involved in VEGF expression in swine ovary GCs

GPER mediates the IL6/JAK2/STAT3 pathway involved in VEGF expression in swine ovary GCs

Histological classification of canine ovarian cyst types with reference to medical history

Lectin histochemical pattern on the normal and cystic ovaries of sows

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