Immunohistochemical Expression of Estrogen Receptor β in Normal and Tumoral Canine Mammary Glands

Mulas, J. Martı́n de las; Ordás, J.; Millán, M. Y.; Chacón, Fernando; Lara, M. De; Monteros, A. Espinosa de los; Reymundo, C.; Jover, Antonio

doi:10.1354/vp.41-3-269

Cited by 29 publications

(32 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the bitch, a study has demonstrated that ERβ is expressed in normal and neoplastic mammary glands, with higher expression in BMT than in MMT. Furthermore, the expression is higher in complex and mixed histological types of MMT (which are associated with a favorable prognosis) compared to simple types (Martín de las Mulas et al, 2004), showing that ERβ expression may be a favorable prognostic factor.…”

Section: Prognosismentioning

confidence: 97%

Canine Mammary Tumors: Risk Factors, Prognosis and Treatments

2016

View full text Add to dashboard Cite

Mammary tumors are the most common neoplasm in the female dog, with a median age of appearance between 9 and 11 years. They may appear as single or multiple nodules, and posterior mammary glands are more frequently affected than anterior glands. Both benign and malignant tumors may occur in the dog, and according to histological criteria, approximately 50% of the tumors are malignant. Mammary gland tumors tend to be heterogeneous in their pathological characteristics and clinical behavior. Different hormones and growth factors play a key role in the development of this neoplasm, however, the mechanism by which they influence tumor growth and their possible prognostic value are still under study. Besides, new therapeutic options for each particular tumor type are being developed. The aim of this article is to review pathological, prognostic and therapeutic aspects of canine mammary neoplasms.

show abstract

Section: Prognosismentioning

confidence: 97%

Canine Mammary Tumors: Risk Factors, Prognosis and Treatments

2016

View full text Add to dashboard Cite

show abstract

“…Martín De las Mulas and coworkers [9] studied the immunohistochemical detection of ER β in normal and tumor-affected canine mammary glands. They reported the expression of ER β in the nuclei of acinar and ductal epithelial cells and some basal myoepithelial cells.…”

Section: Introductionmentioning

confidence: 99%

An Immunohistochemical Study on the Expression of Sex Steroid Receptors in Canine Mammary Tumors

Louis¹,

Varshney²,

Nair³

2012

ISRN Veterinary Science

View full text Add to dashboard Cite

Steroid hormones are found to play a major role in the genesis and progression of mammary tumors. The aim of this study was to immunohistochemically detect the presence of estrogen receptor alpha (ERα), estrogen receptor beta (ERβ), and progesterone receptor (PR) and also to study the association between these markers in 29 cases of benign (11) and malignant (18) canine mammary tumors. ERα immunostaining was noticed in only one case of carcinosarcoma specifically in the nuclei of epithelial and a few myoepithelial cells. ERβ immunostaining was noticed in the nuclei and cytoplasm of epithelial cells and smooth muscles lining the blood vessels. Immunoexpression of ERβ was 82% in benign tumors and 78% in malignant tumors. PR immunostaining was expressed in the nuclei of epithelial cells in both benign and malignant tumors. Among the 15 PR+ cases, 6 (55%) were of benign type, and 9 (50%) were of malignant type. The most common group of hormone receptor was the ERα−/PR+/ERβ+ (46%) in benign tumors and ERα−/PR−/ERβ+ (38%) in malignant tumors. Although there was no significant association between ERα and PR with ERβ, the findings indicated that ERβ was consistently expressed in both benign and malignant tumors, irrespective of ERα and PR status.

show abstract

“…An increased expression for the oestrogen (ER) and progesterone (PR) receptors is related to a better prognosis while the positivity of the CK5 receptor is related to a worse prognosis, in both canines and humans with mammary carcinomas (Terra et al 2012, Horta et al 2012, Figueiroa et al 2012. In canines, the expression of ER is higher in benignant tumors than in malignant ones and is also higher in complex and mixed tumors in comparison with simple sub-types (De Las Mulas et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Cell block sensitivity for immunohistochemical detection of cytokeratin 5, oestrogen and progesterone receptors in canine primary mammary carcinoma

Silveira

Campos

Düfloth

et al. 2017

Austral j. vet. sci.

View full text Add to dashboard Cite

ABSTRACT. Mammary carcinomas are relatively common ailments among female canines aged around 10 years old, presenting an important morbidity with an average survival of five years. The cytoinclusion technique is frequently employed in human medicine as the investigative method of choice as it quickly provides resources for the determination of the correct therapeutic response, however, the effectiveness of the technique in canines remains understudied in veterinary medicine. This study aims at evaluating the degree of correlation with immunohistochemical marking for cytokeratin 5 (CK5), oestrogen and progesterone receptors (ER and PR) between the cytoinclusion and the histopathology technique in mammary carcinomas. Twenty-five samples of mammary carcinoma, both for the cytoinclusion and histopathological techniques were submitted for histological processing; microscope slides were created for hematoxylin-eosin (HE) staining and the immunohistochemical technique (IHC) was assessed for the ER, PR and CK5 receptors. Through the HE staining, we reached a concordance rate of 100% between the cytoinclusion and the histopathological analysis in the diagnosis of carcinomas. The immunohistochemical assay presented sensitivity of 85.71%, 95.45% and 100% and Cohen's kappa of 0.78, 0.84 and 0.95 for ER, PR and CK5, respectively, as well as 100% specificity and P<0.01 for all three markers. Therefore, cytoinclusion represents an accessible, fast and low-cost method, offering high sensitivity for the cytomorphological and immunohistochemical diagnosis of mammary carcinoma in female canines.Key words: cytoinclusion, histopathological, immunohistochemistry, tumor. RESUMEN.Los carcinomas mamarios son relativamente comunes en hembras caninas con edades de alrededor 10 años y representan un importante factor de morbilidad para estas hembras; la técnica de citoinclusión es frecuentemente empleada en medicina humana como un método de diagnóstico que permite proveer información rápida para la determinación de una conducta terapéutica correcta, sin embargo, la efectividad de esta técnica permanece poco estudiada en medicina veterinaria. El objetivo del estudio fue evaluar el grado de correlación entre marcadores inmunohistoquímicos para citokeratina 5 (CK5), receptores de estrógenos y progesterona (RE y RP) entre la técnica de citoinclusión y la técnica histopatológica en carcinoma mamario. Veinticinco muestras de carcinoma mamario para la técnica de citoinclusión e histopatológica fueron sometidas a procesamiento histológico, láminas fueron confeccionadas para la tinción mediante hematoxilina y eosina (HE), y la técnica de inmunohistoquímica para identificar la expresión de RE, RP y CK5. Mediante la tinción de la HE fue encontrado una proporción de concordancia del 100% entre el análisis de citoinclusión e histopatológico de carcinoma mamario. El análisis inmunohistoquímico evidenció una sensibilidad del 85,71%, 95,45% y 100% y un índice kappa de Cohen de 0,78, 0,84 y 0,95 para RE, RP y CK5, respectivamente, así como 100% de especifi...

show abstract

Immunohistochemical Expression of Estrogen Receptor β in Normal and Tumoral Canine Mammary Glands

Cited by 29 publications

References 10 publications

Canine Mammary Tumors: Risk Factors, Prognosis and Treatments

Canine Mammary Tumors: Risk Factors, Prognosis and Treatments

An Immunohistochemical Study on the Expression of Sex Steroid Receptors in Canine Mammary Tumors

Cell block sensitivity for immunohistochemical detection of cytokeratin 5, oestrogen and progesterone receptors in canine primary mammary carcinoma

Contact Info

Product

Resources

About