Immunohistochemical expression of Epstein-Barr virus-encoded latent membrane protein (LMP-1) in paraffin sections of EBV-associated nasopharyngeal carcinoma in Spanish patients

Vera-Sempere, Francisco; Burgos, Javier S.; Botella, Maria-Soledad; Córdoba, Juan; Gobernado, M

doi:10.1016/0964-1955(95)00093-3

Cited by 29 publications

(16 citation statements)

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“…Niedobitek et al observed that EBER was exclusively present in the UCNT tumors 7, 39. In a fourth cohort of Spanish patients with NPC, EBV gene products were present in all 51 samples, with LMP‐1 present in 78% of patients; however, no association was observed between LMP‐1 and the WHO classification of tumor sub‐type 40…”

Section: Discussionmentioning

confidence: 95%

Molecular pathology parameters in human nasopharyngeal carcinoma

Shi

Pataki

MacMillan

et al. 2002

Cancer

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BACKGROUND To derive a better understanding of the biologic behavior of nasopharyngeal carcinoma (NPC), the authors evaluated a number of molecular variables to address the hypothesis that p53 dysfunction in NPC is associated with Epstein‐Barr virus (EBV), increased tumor angiogenesis, lower likelihood of apoptosis, and poorer clinical outcome. MATERIALS The biopsy samples from 87 NPC patients were obtained and sections were made to detect EBV, using in‐situ hybridization; the authors used immunohistochemistry to assess p53, p21WAF1/CIP1 expression, and microvessel density count (MVD). In situ end labelling was used to evaluate apoptosis and necrosis. Analyses were conducted on the association between each of these variables as well as clinical outcome, including survival and local control. RESULTS There was a highly significant association between EBV‐encoded RNA (EBER) positivity with p53 over‐expression in that only 1 out of 32 p53 over‐expressing tumors was EBER negative, as opposed to 19 out of 48 p53 negative tumors being EBER negative (P = 0.001). In addition, EBER positivity was highly associated with World Health Organization (WHO) type 3 NPC, Asian/Chinese ethnicity, a lower apoptotic index, and p21 over‐expression. p53 over‐expression was associated with a higher MVD count. Controlling for age and nodal status, EBER positivity was associated with both improved overall survival (P = 0.02), and disease‐free survival (P = 0.04). In contrast, the presence of tumor necrosis was associated with an inferior local control (P = 0.03). CONCLUSION p53 protein was over‐expressed in approximately one third of NPC samples in the current study, and this correlated significantly with the presence of EBER. Epstein‐Barr virus status was also associated with WHO type 3 NPC, Asian/Chinese ethnicity, and induction of p21. The presence of EBV appeared to predict for improved survival, the mechanism of which remains to be elucidated in this biologically complex disease. Cancer 2002;94:1997–2006. © 2002 American Cancer Society. DOI 10.1002/cncr.10440

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Section: Discussionmentioning

confidence: 95%

Molecular pathology parameters in human nasopharyngeal carcinoma

Shi

Pataki

MacMillan

et al. 2002

Cancer

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“…7,39 In a fourth cohort of Spanish patients with NPC, EBV gene products were present in all 51 samples, with LMP-1 present in 78% of patients; however, no association was observed between LMP-1 and the WHO classification of tumor sub-type. 40 The only other group which scored apoptosis in NPC observed a higher AI associated with p53 overexpression and a lower clinical stage. 5 Our patient samples showed an inverse relationship between positive EBER status and a lower AI.…”

Section: Discussionmentioning

confidence: 96%

Metastatic nonseminomatous germ cell tumors of the testis

Hendry

Norman

Dearnaley

et al. 2002

Cancer

138

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Corrugated plate photocatalytic reactors provide a promising photoreactor design due to its potential in enhancing energy efficiency. A detailed model of such reactors is needed for engineering applications and process design. The model for the light absorption in a corrugated plate reactor using a nonuniform radiation source is developed based on first principles. The local area‐specific rate of energy absorption (LASREA) distribution on the corrugated plates is examined via simulation. The effect of the design parameters on LASREA is investigated. The results from this research provide feasible basis for design and optimization of corrugated plate photoreactors. © 2005 American Institute of Chemical Engineers AIChE J, 2005

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“…These genes include three latent membrane proteins 1, 2A, and 2B (LMPs), six EBV nuclear antigens (EBNA1, 2, 3A, 3B, 3C, and EBNA-LP), and two small, non-coding nuclear RNAs (EBERs). Of these, LMP1 is oncogenic and was reported to be expressed in more than 70% of NPC patients [6,7]. …”

Section: Introductionmentioning

confidence: 99%

Epstein-Barr virus Latent Membrane Protein LMP1 reduces p53 protein levels independent of the PI3K-Akt pathway

2011

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BackgroundNasopharyngeal carcinoma (NPC) is an epithelial malignancy, which commonly occurs in Southern China, Taiwan, North Africa and Southeast Asia. Nasopharyngeal carcinoma is strongly associated with Epstein-Barr virus infection. The p53 tumour suppressor protein is rarely mutated in NPC suggesting that the inactivation of p53 pathway in NPC could be due to the presence of EBV proteins. The aim of this work was to determine the effects of EBV proteins namely LMP1 and LMP2A on the expression levels of p53 protein.FindingsIn this work we found that LMP1, but not LMP2A, decreased p53 protein levels. Overexpression of LMP1 resulted in increased ubiquitination of p53 suggesting that the decreased p53 protein levels by LMP1 was due to increased degradation of the protein. The reduction of p53 protein levels was independent of the PI3K-Akt pathway.ConclusionsLMP1, but not LMP2A, reduced p53 protein levels through the increase in the polyubiquitination of p53 protein and was independent of the PI3K-Akt pathway.

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Immunohistochemical expression of Epstein-Barr virus-encoded latent membrane protein (LMP-1) in paraffin sections of EBV-associated nasopharyngeal carcinoma in Spanish patients

Cited by 29 publications

References 24 publications

Molecular pathology parameters in human nasopharyngeal carcinoma

Molecular pathology parameters in human nasopharyngeal carcinoma

Metastatic nonseminomatous germ cell tumors of the testis

Epstein-Barr virus Latent Membrane Protein LMP1 reduces p53 protein levels independent of the PI3K-Akt pathway

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