Immunohistochemical expression levels of p53 and eIF4E markers in histologically negative surgical margins, and their association with the clinical outcome of patients with head and neck squamous cell carcinoma

Singh, Jagtar; Jayaraj, Ramamoorthi; Baxi, Siddhartha; Mileva, Mariana; Skinner, John M.; Dhand, Navneet K.; Thomas, Mahiban

doi:10.3892/mco.2015.689

Cited by 22 publications

(28 citation statements)

References 21 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to compare the correlation between Thsd7a and common markers of ESCC immunohistochemistry, we performed 4 other indicators of the same patient group (P53, P63, CK5/6, and Ki-67; Figure 1B ). The positive rates and expression localization of these 4 indexes were consistent with previous studies [ 25 – 29 ], while the positive rate of Thsd7a for ESCC and adjacent normal tissue was 70.1% and 18.2%, respectively (Table 1 ). And the expression of these indicators, suggesting that the cancer of these patients had the characteristic of active proliferation and highly malignant degree of ESCC.…”

Section: Resultssupporting

confidence: 90%

“…Thsd7a is mainly expressed in cell membrane, which is entirely concordant with the previous findings in Membranous Nephropathy [ 23 , 24 ], and there is a little expression in the cytoplasm, which was never sported before. We used P53, P63, CK5/6, Ki-67 as positive control, which showed that the tumor tissues collected were higher malignant ESCC, and the positive rates of the markers were similar with the early reports [ 25 – 29 ]. The positive rate (70.1%) and positive coincidence rate (55.6%) showed that Thsd7a had higher expression in ESCC of Kazakh patients, Xinjiang.…”

Section: Discussionsupporting

confidence: 71%

See 1 more Smart Citation

Expression, prognosis and functional role of Thsd7a in esophageal squamous cell carcinoma of Kazakh patients, Xinjiang

et al. 2017

View full text Add to dashboard Cite

Thsd7a (Thrombospondin type 1 domain containing 7a) is a critical transmembrane protein. Studies have indicated that Thsd7a was associated with cytoskeletal organization, cell migration and filopodia formation. However, the involvement of Thsd7a remains elusive in human Esophageal Squamous Cell Carcinoma (ESCC). Consequently, immunohistochemistry and reverse transcription-polymerase chain reaction were utilized to study the correlation between the expression of Thsd7a and clinical-pathological characteristics. The influence of Thsd7a on apoptosis, cell proliferating activity, cell cycle, migratory and invasive capacity was determined in Eca 109 and EC 9706 cell lines in vitro. And the influence on proliferating activity was testified using naked mice model in vivo. In addition, the potential molecular mechanism was tested by microarray. It was discovered that there is a certain correlation between Thsd7a and the Kazakh ESCC. By knocking out Thsd7a, the invasion, migration and proliferation could be decreased. And it could also arrest the cell cycle at G1 phase and increase the apoptosis rate. It was further verified that Thsd7a had obvious effect on proliferation in naked mice with xenograft of Eca109 cells. Finally, it was uncovered by microarray analysis that a variety of tumor genes and pathways related to Thsd7a. Together, it was demonstrated that Thsd7a might have a certain degree of carcinogenesis in ESCC.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Discussionsupporting

confidence: 71%

Expression, prognosis and functional role of Thsd7a in esophageal squamous cell carcinoma of Kazakh patients, Xinjiang

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The phosphorylation of EIF4E is very frequently observed in HNSCC [49]. Moreover, EIF4E is up-regulated in surgical margins of HNSCC patients with local recurrence and could serve as a prognostic biomarker [53]. DeFatta et al indicated that the FaDu cell line displays a high EIF4E protein level and that this is similar to the level observed in patients.…”

Section: Discussionmentioning

confidence: 98%

Oncogenic Role of ZFAS1 lncRNA in Head and Neck Squamous Cell Carcinomas

Kolenda

Guglas

Kopczyńska

et al. 2019

Cells

View full text Add to dashboard Cite

Background: Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous disease with high mortality. The identification of specific HNSCC biomarkers will increase treatment efficacy and limit the toxicity of current therapeutic strategies. Long non-coding RNAs (lncRNAs) are promising biomarkers. Accordingly, here we investigate the biological role of ZFAS1 and its potential as a biomarker in HNSCC. Methods: The expression level of ZFAS1 in HNSCC cell lines was analyzed using qRT-PCR. Based on the HNSCC TCGA data, the ZFAS1 expression profile, clinicopathological features, and expression of correlated genes were analyzed in patient tissue samples. The selected genes were classified according to their biological function using the PANTHER tool. The interaction between lncRNA:miRNA and miRNA:mRNA was tested using available online tools. All statistical analyses were accomplished using GraphPad Prism 5. Results: The expression of ZFAS1 was up-regulated in the metastatic FaDu cell line relative to the less aggressive SCC-25 and SCC-040 and dysplastic DOK cell lines. The TCGA data indicated an up-regulation of ZFAS1 in HNSCCs compared to normal tissue samples. The ZFAS1 levels typically differed depending on the cancer stage and T-stage. Patients with a lower expression of ZFAS1 presented a slightly longer disease-free survival and overall survival. The analysis of genes associated with ZFAS1, as well its targets, indicate that they are linked with crucial cellular processes. In the group of patients with low expression of ZFAS1, we detected the up-regulation of suppressors and down-regulation of genes associated with epithelial-to-mesenchymal transition (EMT) process, metastases, and cancer-initiating cells. Moreover, the negative correlation between ZFAS1 and its host gene, ZNFX1, was observed. The analysis of interactions indicated that ZFAS1 has a binding sequence for miR-150-5p. The expression of ZFAS1 and miR-150-5p is negatively correlated in HNSCC patients. miR-150-5p can regulate the 3′UTR of EIF4E mRNA. In the group of patients with high expression of ZFAS1 and low expression of miR-150-5p, we detected an up-regulation of EIF4E. Conclusions: In HNSCC, ZFAS1 displays oncogenic properties, regulates important processes associated with EMT, cancer-initiating cells, and metastases, and might affect patients’ clinical outcomes. ZFAS1 likely regulates the cell phenotype through miR-150-5p and its downstream targets. Following further validation, ZFAS1 might prove a new and valuable biomarker.

show abstract

“…10 p53 mutations in negative surgical margins have been shown to be valuable in predicting local recurrences in head and neck squamous cell carcinoma. [11][12][13] Eukaryotic initiation factor 4E (eIF4E) have a crucial role in control of initial stage protein synthesis. 14 It is the product of a proto-oncogene and carries immense potential as a tumor marker as it has shown overexpression in several epithelial cell tumors and has been reported to be almost 100% overexpressed in HNSCC, breast carcinomas and colon carcinomas.…”

Section: Introductionmentioning

confidence: 99%

“…15,16 Few retrospective studies have shown that expression of eIF4E at negative surgical margins of HNSCC is useful in predicting local relapse. 13,17 E-cadherin is important for development and maintenance of epithelium and is one of the most essential molecules in cell-to-cell adhesion. 18 The deficit of E-cadherin-mediated cell adhesion associates with the loss of the epithelial morphology and results in invasion of tumor cells.…”

Section: Introductionmentioning

confidence: 99%

The prognostic role of immunohistochemical markers in surgical margins of Indian patients with oral squamous cell carcinoma- a retrospective study protocol

Ranka¹,

Chaudhary²,

Sharma³

et al. 2021

Int J Clin Trials

View full text Add to dashboard Cite

Background: Positive surgical margins in oral squamous cell carcinoma (OSCC) is an important predictor of recurrence. Unfortunately, recurrence is found in cases with negative surgical margins as well and hence molecular markers are being investigated to identify the tumor cells in negative surgical margins to improve the survival of patients. The study aimed to ascertain the prognostic role of p53, eIF4E and E-cadherin in negative surgical margins and association of their expression with clinical parameters, recurrence, and survival.Methods: India based retrospective cohort study of patients who were surgically treated for OSCC between 2011 and 2014. Thirty patients with negative surgical margins were assessed for this study. Sixty-four negative surgical margins were evaluated using immunohistochemistry with monoclonal mouse p53, monoclonal rabbit eIF4E and monoclonal mouse E-cadherin antibodies.Results: Fisher’s exact test, Fishers Freeman Halton exact test and contingency tables were used to assess the association between recurrence rate and clinicopathologic parameters also any association with the expression of p53, eIF4E and E-cadherin in the surgical margins and each patients’ clinical characteristics. ANOVA (analysis of variance) was used to assess the role of clinicopathologic parameters in survival of the patients. The sensitivity and specificity of each antibody along with positive and negative predictive values were calculated. Kaplan Meier was used for analysis of recurrence and five-year survival. P<0.05 was used to denote a statistically significant difference.Conclusion: This study could help in identification of patients with minimal residual cancer in the surgical margins and might help in better therapeutic management to improve survival of OSCC patients.

show abstract

Immunohistochemical expression levels of p53 and eIF4E markers in histologically negative surgical margins, and their association with the clinical outcome of patients with head and neck squamous cell carcinoma

Cited by 22 publications

References 21 publications

Expression, prognosis and functional role of Thsd7a in esophageal squamous cell carcinoma of Kazakh patients, Xinjiang

Expression, prognosis and functional role of Thsd7a in esophageal squamous cell carcinoma of Kazakh patients, Xinjiang

Oncogenic Role of ZFAS1 lncRNA in Head and Neck Squamous Cell Carcinomas

The prognostic role of immunohistochemical markers in surgical margins of Indian patients with oral squamous cell carcinoma- a retrospective study protocol

Contact Info

Product

Resources

About