Immunohistochemical examination of cholecystokinin and gastrin receptors (CCK-2/gastrin-R) expression in normal and exocrine cancerous human pancreatic tissues

Sellam, Feriel; Harir, Noria; Khaled, Meghit Boumédiène; Mrabent, Nesrine M.; Belkralladi, Houria; Tou, Abdelnacer; Diaf, Mustapha; Salah, Rachida; Moulessehoul, Soraya

doi:10.1016/j.pan.2015.09.019

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…Various techniques as RT-PCR and western blotting were used to treatment and diagnosis in exocrine pancreatic cancer. [22][23] GRM8, which was downregulated in active group was shown to be one of the most important receptors having a role in L-glutamate neurotransmitter transfer in the central nervous system. Glutamatergic neurotransmitters were shown to be vital components of normal brain functions.…”

Section: Discussionmentioning

confidence: 99%

Comparative Expression Analysis of Genes Encoding Neurotransmitter Receptors Between Entrepreneurs and Non-Entrepreneurs

Yıldırım¹,

Aslan²,

Daştan³

et al. 2021

PJMHS

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Introduction and Purpose: Recent studies revealed that different behavioral and physiological processes are closely related to different receptor levels in humans. On the other hand, there weren’t any studies that investigated neurotransmitter activity and entrepreneurship relationships. Little is known about how genes expressed in the blood are associated with entrepreneurship. In this study, we measured the expression of 84 genes encoding neurotransmitter receptors between the entrepreneurs and non-entrepreneurs (n=25) using RT-PCR arrays to monitor differentially expressed genes for exploring molecular mechanism behind the entrepreneurship mindset. Materials and Methods: After determining whether participants are entrepreneurs or not, blood samples were collected. Blood samples were collected in Vacutainer® EDTA tubes as 10 ml and RNA isolation was performed at the Erzurum Technical University, Cell Culture Laboratory. Trizol® solution for RNA isolation (Thermo®, USA) was applied according to the manufacturer's instructions. Concentrations of RNA samples were measured at 260 nm using a spectrophotometer (Epoch®, Biotek). Then, total RNA was reverse transcribed into cDNA by using High-Capacity cDNA Reverse Transcription Kit (Thermo®). Total 84 genes were analyzed via RT² Profiler™ Human Neurotransmitter Receptors PCR Array (Qiagen®, USA) in Rotor-Gene Q real-time PCR cycler (Qiagen®, USA). Findings: The gene expression results obtained from RT-PCR were compared between entrepreneurs and non-entrepreneurs, and presented as Fold Changes (FC). According to our results, positive FC values indicated an increase in the expression of the genes and negative FC values indicated decrease in gene expression levels in entrepreneurs. Results: These 84 different genes regulate neurotransmitter biosynthesis, uptake, transport, and signaling via neurotransmitter receptors. According to gene expression analyses, gene expressions that could be related to the entrepreneur behavior might be connected to not only undesired psychological outcomes like various addictions and also neurological cases such as schizophrenia and depressive disorder. Our results firstly indicated that entrepreneurship was not only associated with neurotransmitter release but also with receptor levels. Keywords: Entrepreneurship, Entrepreneurs, Genes, Neurotransmitter Receptors, RT-PCR Array

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Section: Discussionmentioning

confidence: 99%

Comparative Expression Analysis of Genes Encoding Neurotransmitter Receptors Between Entrepreneurs and Non-Entrepreneurs

Yıldırım¹,

Aslan²,

Daştan³

et al. 2021

PJMHS

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“…The other important finding was that cholecystokinin A and B receptors are differentially expressed in normal pancreas and pancreatic adenocarcinoma. It was demonstrated that the human CCK-A receptor is selectively expressed by pancreatic adenocarcinoma, but not by normal pancreas [15][16][17].…”

Section: Discussionmentioning

confidence: 99%

Biochemical Clinical and Pathological Profile of Pancreatic Head Carcinoma in North Africa

Diallo¹,

Konate²,

Sellam³

et al. 2020

JSO

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Objective: A statistical clinical assessment of the effects of bilirubin and others hem catalyze biochemical parameters over the main signaling pathways involved in pancreatic head cancer to determine their carcinogenic impact using a molecular approach. Material and Methods: Patients’ data were collected at surgery department Sidi bel Abbes University Hospital (North Africa) patients were diagnosed with pancreatic cancer between 2013 and 2016. The data inclusion criteria were: Age, gender, medical history, surgical history, the year of diagnosis, as well as biological records such as: bilirubin, fibrinogen, creatinine and urea rates. Results: 44 patients with pancreatic cancer were included in our study (sex ratio=1.75). The mean age was 67.61 years. The most recorded medical history was diabetes with a rate of 43.2%, concerning surgical histories, 27.3% patients underwent a cholecystectomy. M1 was the most common diagnosis stage (27%). 81.81% of patients had hyperbilirubinemia, 6.81% had a normal bilirubinemia, 25% had high level of fibrinogen, 3.6% had a normal level. 77.3% had normal ureanemia, 18.2% had hyper-ureanemia and 15.9% had hypocreatininemia, 68.2% had normal level, 13.6% had hypercreatininemia. We matched patients’ groups with bilirubin level using Spearman’s correlation test and found a significant P value (P=0.015). We calculated the correlation between bilirubinemia and the stage of diagnosis, using the one-way ANOVA test and we found a significant P value (P=0.013). Conclusion: Our results represent an interesting shortcut to synthetize specific biochemical antibodies for bio-marking and diagnosis use, or even to lower serum level to reduce cancer aggressiveness.

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Role of p38 Signaling Pathway in Pentagastrin-Regulated Cell Proliferation of Colorectal Carcinoma Cell Line HT-29

Mao¹,

Wu²,

Wu³

et al. 2016

JCT

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Objective: To investigate the effects and mechanisms of p38 signaling pathway in pentagastrinregulated cell proliferation of colorectal carcinoma cell line HT-29. Methods: HT-29 cell line of colorectal carcinoma was in vitro incubated and divided into the control group, pentagastrin group, proglumide group, and pentagastrin + proglumide group. MTT reduction assay was performed to detect the proliferation status of HT-29 cell line and determine the optimal dosage of pentagastrin and proglumide. Annexin V-fluorescein isothiocyanate flow cytometry was used to detect the proliferation index (PI) and apoptosis rate (AR) of HT-29 cells. Reverse transcriptase polymerase chain reaction was performed to detect the mRNA expression of the pentagastrin receptor/cholecystokinin-B receptor (CCK-BR) and p38. The protein and phosphorylation levels of p38 were estimated by western blotting. Results: RT-PCR detection showed that CCK-BR mRNA was expressed in the HT-29 cell line. Pentagatrin improved HT-29 cell proliferation in dosage of 6.25 -100 mg/L, and the optimal dosage of pentagastrin was 25.0 mg/L. Proglumide had no significant effect on the proliferation of HT-29 cells, but significantly inhibited the proliferation of HT-29 cells stimulated by pentagastrin when the dosage of proglumide was 8.0 -128.0 mg/L, and the optimal dosage was 32.0 mg/L. The AR in the pentagastrin group was significantly lower than that in the control group and in the pentagastrin + proglumide group. The PI in the pentagastrin group was significantly higher than that in the control group and in the pentagastrin + proglumide group. P38 phosphorylation level in the pentagastrin group was significantly lower than that in the control group, and in the pentagastrin + proglumide group. There were no significant differences in the mRNA and protein expression of p38 in the control, pentagastrin, proglumide and pentagastrin + proglumide groups. Conclusion: Pentagastrin can improve proliferation of the CRC cell line HT-29 and inhibit apoptosis via the p38 signal transduction pathway. This mechanism may be associated with suppressed p38 protein phosphorylation level due to inhibition of proglumide, a ga-J. D. Mao et al. 379 strin receptor antagonist.

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Immunohistochemical examination of cholecystokinin and gastrin receptors (CCK-2/gastrin-R) expression in normal and exocrine cancerous human pancreatic tissues

Cited by 3 publications

References 46 publications

Comparative Expression Analysis of Genes Encoding Neurotransmitter Receptors Between Entrepreneurs and Non-Entrepreneurs

Comparative Expression Analysis of Genes Encoding Neurotransmitter Receptors Between Entrepreneurs and Non-Entrepreneurs

Biochemical Clinical and Pathological Profile of Pancreatic Head Carcinoma in North Africa

Role of p38 Signaling Pathway in Pentagastrin-Regulated Cell Proliferation of Colorectal Carcinoma Cell Line HT-29

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