Immunohistochemical evidence of Muc1 expression during rat embryonic development

Abstract

During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (−), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.

Preparation, quality control, and biodistribution assessment of [111In]In‐DOTA‐PR81 in BALB/c mice bearing breast tumors

“…Also high uptake in kidney organ during the time study were observed which is in agreement with the findings of Lacunza and coworkers 17 . This has already been shown by other groups, working with anti‐MUC1 probes 39–41 …”

Deficiency of intestinal mucin-2 ameliorates experimental alcoholic liver disease in mice

“…The negative control was performed using isotype immunoglobulin G instead of the primary antibody. The positive control is small intestine from a 2‐week‐old male C57BL/6J mouse 47. Representative sections are shown.…”

Spatiotemporal expression of Rhomboid domain containing 2 (Rhbdd2) during rat development

“…Embryos and fetuses were obtained and processed according to previous studies (Lacunza et al, 2010). They were obtained by laparotomy and hysterectomy of the pregnant females.…”

“…Immunohistochemistry was performed according to standard protocols as reported in a previous study (Lacunza et al, 2010). Briefly, dewaxed sections were treated with 10 mM sodium citrate buffer at 100 • C for 5 min for antigen retrieval and were placed in methanol with 0.3% H 2 O 2 to block endogenous peroxidase activity; after three washes in PBS, sections were blocked for non-specific binding with normal horse serum diluted 1:10 in 1% bovine serum albumin in PBS, (BSA)/PBS.…”