Immunohistochemical evidence of leptin and its receptor in the carpal glands of domestic pigs and wild boar

Dall’Aglio, Cecilia; Mercati, Francesca; Pascucci, Luisa; Bazzucchi, Cinzia; Ceccarelli, Piero

doi:10.1111/vde.12175

Cited by 2 publications

(2 citation statements)

References 9 publications

(17 reference statements)

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“…In this regard, it is well known that many other hormones known as adipokines are produced not only by white adipose tissue but also by other organ tissues, where they probably play different roles, always linked to the functionality of the tissue/organ where they are evidenced. [1][2][3][4][5][6] Resistin is an adipose tissue-specific secretory factor that in humans is encoded by the RETN gene. 7 The first study considering resistin as an adipokine was on mice, in which the molecule induced resistance to insulin (its name is most likely linked to this particular action); hence, it was perceived as a potential and dangerous link between obesity and diabetes.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results

et al. 2019

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Resistin is a polypeptide hormone of the adipokine-family, primarily, but not exclusively, produced by the adipose tissue. Recent studies suggested that resistin may affect the male and female reproductive activity. The study aim was to immunohistochemically evaluate the presence and distribution of resistin in the ovine uterus. Uterine samples were collected from two groups of ewes at the end of an experimental trial during which the animals of the first group (CTRL) were fed only by grazing while those of the second one (EXP) were supplemented with barley and corn. Using a monoclonal antibody against resistin, tested by Western Blot, the immunopositive reaction was identified in the cytoplasm of epithelial lining cells and uterine glands. The endogenous production of resistin seemed to be affected by different diet, as evidenced by staining differences between the CTRL and EXP groups. Our findings support the existence of a peripheral resistin system in the sheep uterus. It is possible that this system is involved in the functionality of the uterus, which is also affected by the animal’s nutritional status.

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Section: Introductionmentioning

confidence: 99%

Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results

et al. 2019

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“…In addition, some authors showed the primary action of intradermal adipose tissue (IAT) in mouse and humans as a key source of signaling molecules and adipokines, including Lep, that intervene in regulating HF cycle. 11,12 Following studies on humans and laboratory animals, investigation on this molecule has also been performed in domestic animals, 13,14 although research on the skin is still limited. 15…”

Section: Introductionmentioning

confidence: 99%

Epithelial expression of the hormone leptin by bovine skin

et al. 2019

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Leptin (Lep) stimulates keratinocytes to proliferate, intervenes in the wound healing and participates to hair follicle morphogenesis and cycle. While it is secreted by skin structures including epidermis and hair follicles, intradermal adipose tissue also seems to have a role in Lep secretion and accordingly in the control of hair follicle growth in mice and humans. Lep was investigated in the skin of humans and laboratory animals but there are not data regarding bovine species. The aim of this work was to study the expression of Lep and its receptor (LepR) in the skin of bovine and, at the same time, to investigate the presence and extension of intradermal adipose tissue. A morphological evaluation of the skin was performed while the presence and localization of Lep and LepR were analyzed by RTPCR and immunohistochemistry. A high and thick dermis without adipocytes was observed. Hair follicles and sebaceous and sweat glands were located in the proximal part of the skin while a thick layer of connective tissue, lacking adipose cells, separated these structures by subcutis. RT-PCR evidenced the transcripts for both molecules. By immunohistochemistry, Lep and LepR were observed in the epidermis and hair follicles. Based on the absence of intradermal adipose tissue and the presence of both Lep and LepR in the epidermis and in the hair follicle epithelium, it can be posited that in bovine skin Lep participates to the control of epidermis growth and hair follicle cycle through a paracrine and autocrine mechanism.

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Immunohistochemical evidence of leptin and its receptor in the carpal glands of domestic pigs and wild boar

Abstract: We hypothesize that leptin may be produced by the carpal gland and may act on the gland's secretory epithelial cells with an autocrine/paracrine mechanism, thus affecting the secretory activity of the gland itself.

Cited by 2 publications

References 9 publications

Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results

Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results

Epithelial expression of the hormone leptin by bovine skin

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