Immunohistochemical Evaluation of the Effects of Nitrate in Drinking Water on Laminin Alpha-5 Expression in Rat Renal Glomeruli

Jalali, Mehdi; Nikravesh, Mohammad Reza; Dorafshani, Mahmoud Moghaddam

doi:10.5812/ircmj.42638

Cited by 2 publications

(3 citation statements)

References 30 publications

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“…Also, there was no significant difference in lamininα 5 expression among the group receiving water containing 45mg/L of nitrate compared to distilled water and control groups. However, they observed a significant increase in anti-lamininα 5 reaction in a group treated with water containing 100mg/L of nitrate compared to another group [25]. These results were not in line with our findings regarding the effects of hyperthyroidism on lamininα 5 gene expression.…”

Section: Discussioncontrasting

confidence: 99%

“…Mohammadi et al in a study using a mouse model showed that nicotine-induced immunohistochemical reaction of collagen IV in the alveolar BM of the control group changed from light brown to dark brown [23], and in a similar investigation on pulmonary alveoli, laminin expression significantly decreased in experimental groups compared to control groups [24]. Effects of nitrate in drinking water on laminin-α5 expression in rat renal glomeruli showed that by increasing the concentration of nitrate in drinking water of Wistar rats, the severity of the reaction of anti-laminin-α5 decreased in renal glomeruli [25]. Also, there was no significant difference in lamininα 5 expression among the group receiving water containing 45mg/L of nitrate compared to distilled water and control groups.…”

Section: Discussionmentioning

confidence: 90%

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Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-Î±5 Gene Expression in Balb/C Mouse Seminiferous Tubules

Sadeghi,

Jalali,

Nikravesh

et al. 2019

GMJ

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Background: Hyperthyroidism is one of the disorders of the thyroid gland, an organ that controls the cellular and molecular behaviors of the seminiferous tubule basement membrane (BM), and ultimately, influences its evolutionary process. We aimed to investigate the effects of hyperthyroidism on immunohistochemical characteristics and gene expression levels of collagen IV and laminin-Î±5 in seminiferous tubules BM of Balb/C mice. Materials and Methods: Twenty male Balb/C mice were divided into experimental and control groups. The experimental group received 500 mg/l of levothyroxine (L-thyroxine) diluted in drinking water for two months to inducing hyperthyroidism, which was confirmed by radioimmunoassay. At the end of the study, the mice were sacrificed, and their testes were extracted for immunohistochemistry and real-time polymerase chain reaction assays. Results: Although a weak reaction was observed in the experimental specimens, no significant enhancement was noted in color intensity of type IV collagen in the seminiferous tubules BM of the experimental group as compared to the control group (P>0.05). Collagen IV gene expression results in the experimental group were not significantly different from the controls (P>0.05). Thus, there was a significant increase in laminin Î±5 gene expression compared to the control group (P=0.016). Conclusion: Considering the key role of collagen IV and laminin-Î±5 in the seminiferous tubule BM in the testes, the results of this study indicated that hyperthyroidism has important effects on both structures and functions of these two components. [GMJ.2019;8:e1369]

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 90%

Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-Î±5 Gene Expression in Balb/C Mouse Seminiferous Tubules

Sadeghi,

Jalali,

Nikravesh

et al. 2019

GMJ

View full text Add to dashboard Cite

show abstract

“…in a study using a mouse model showed that nicotine-induced immunohistochemical reaction of collagen IV in the alveolar BM of the control group changed from light brown to dark brown [ 23 ], and in a similar investigation on pulmonary alveoli, laminin expression significantly decreased in experimental groups compared to control groups [ 24 ]. Effects of nitrate in drinking water on laminin-α5 expression in rat renal glomeruli showed that by increasing the concentration of nitrate in drinking water of Wistar rats, the severity of the reaction of anti-laminin-α5 decreased in renal glomeruli [ 25 ]. Also, there was no significant difference in lamininα 5 expression among the group receiving water containing 45mg/L of nitrate compared to distilled water and control groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-α5 Gene Expression in Balb/C Mouse Seminiferous Tubules

Sadeghi

Jalali

Nikravesh

et al. 2019

GMJ

Self Cite

View full text Add to dashboard Cite

Background: Hyperthyroidism is one of the disorders of the thyroid gland, an organ that controls the cellular and molecular behaviors of the seminiferous tubule basement membrane (BM), and ultimately, influences its evolutionary process. We aimed to investigate the effects of hyperthyroidism on immunohistochemical characteristics and gene expression levels of collagen IV and laminin-α5 in seminiferous tubules BM of Balb/C mice. Materials and Methods: Twenty male Balb/C mice were divided into experimental and control groups. The experimental group received 500 mg/l of levothyroxine (L-thyroxine) diluted in drinking water for two months to inducing hyperthyroidism, which was confirmed by radioimmunoassay. At the end of the study, the mice were sacrificed, and their testes were extracted for immunohistochemistry and real-time polymerase chain reaction assays. Results: Although a weak reaction was observed in the experimental specimens, no significant enhancement was noted in color intensity of type IV collagen in the seminiferous tubules BM of the experimental group as compared to the control group (P>0.05). Collagen IV gene expression results in the experimental group were not significantly different from the controls (P>0.05). Thus, there was a significant increase in laminin α5 gene expression compared to the control group (P=0.016). Conclusion: Considering the key role of collagen IV and laminin-α5 in the seminiferous tubule BM in the testes, the results of this study indicated that hyperthyroidism has important effects on both structures and functions of these two components. [GMJ.2019;8:e1369]

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Immunohistochemical Evaluation of the Effects of Nitrate in Drinking Water on Laminin Alpha-5 Expression in Rat Renal Glomeruli

Abstract: Background: Nitrate is a polyatomic ion with the molecular formula NO3 -. Nitrate poisoning occurs through metabolism in the

Cited by 2 publications

References 30 publications

Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-Î±5 Gene Expression in Balb/C Mouse Seminiferous Tubules

Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-Î±5 Gene Expression in Balb/C Mouse Seminiferous Tubules

Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-α5 Gene Expression in Balb/C Mouse Seminiferous Tubules

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