Immunohistochemical evaluation of proliferative activity (Ki-67 index) in different histological types of cutaneous basal cell carcinoma

Abstract

Evaluation of tumor cell proliferation status belongs to the basic prognostic indicators in a routine biopsy report. In cutaneous basal cell carcinoma (BCC), however, there are discrepancies about a true prognostic significance of this histopathological parameter. The aim of this study was to assess a proliferative activity (Ki-67 index) in BCCs of the skin. Biopsy specimens from 80 cutaneous BCCs (63 primary, 17 recurrent) of different histological types from 75 subjects (34 men, 41 women) were enrolled into this study. All samples were immunohistochemically stained by antibody against Ki-67 antigen (DAKO, clone MIB-1, dilution 1:100). For the statistical analysis, χ 2 test was employed. We found a striking percentage variability of nuclear Ki-67 expression in individual tumors (range 2-70%). Mean value of Ki-67 index was 27.4% (in primary tumors 28.1 %, in recurrent lesions 25.6%). The highest Ki-67 expression occurred in infiltrative BCCs (average 38.1%), morpheaform BCCs (average 37.0%), and superficial BCCs (average 35.7%), the lowest expression was recorded in nodular BCCs (average 21.7%) and BCCs with adnexal (trichoepithelial) differentiation (18.6%). There were not persuasive and statistically significant quantitative differences in proliferation activity of tumor cells between the individual histological BCC types, as well as between primary and recurrent lesions. A distribution of Ki-67 positive cells in tumor nests was mostly irregular and areas with a high number of Ki-67 labeled cells often occurred adjacent to areas with a lower number of cells expressing this marker. Because of a marked Ki-67 staining variability, we can conclude that the simple quantification of BCC proliferation activity alone may not be sufficient for the prediction of further biological behavior, evolution and clinical outcome of this malignancy.

In vitro and in vivo antitumor effect of gefitinib nanoparticles on human lung cancer

“…In our study, the GEF-NPs group showed the lowest expression of ki-67, CD-31 and EGFR after treatment compared with the other three groups. A low degree of cell proliferation, MVD and EGFR generally correlates with slow tumor growth, higher degree of differentiation and hence more moderate clinical and biological behavior (Barto s et al, 2012).…”

O Mosaico Inflamatório nos Subtipos de Carcinoma Basocelular

“…entretanto, a média de células em proliferação variou muito de um subtipo ao outro de cBc, apresentando baixa atividade proliferativa celular, apesar da variação. outro estudo 25 indica que um tumor individual não possui um padrão homogêneo de proliferação. o subtipo morfea apresentou a mais alta média de células em proliferação.…”

Evaluation of Topical Application of Platelets Rich Plasma and Hyaluronic Acid on the Healing of Experimentally Induced Tongue Ulcer

“…Immunohistochemical study was done to detect the Ki-67 nuclear antigen to evaluate the cellular proliferation and CD31 to detect the endothelial cells proliferation. Immunohistochemical comprised the following stages (20,21) :…”

“…The Ki-67 labeling index (LI) for cell proliferation was used five areas at high power (×400 magnification). A minimum of 500 cells were calculated, and the percentage among the number of cells that had a brown stained nucleus and the whole number of cells was calculated (20).…”