Immunohistochemical distribution patterns of collagen type II, chondroitin 4-sulfate, laminin and fibronectin in human nasal septal cartilage

Üstünel, İ̇smail; Çaylı, Sevil; Güney, Kenan; Çelik-Özenci, Çiler; Tanrıöver, Gamze; Sahin, Zeliha; Balkan, Esor; Demir, Ramazan

doi:10.1078/0065-1281-00699

Cited by 13 publications

(11 citation statements)

References 20 publications

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“…This occurred when ossification of the laryngeal skeleton had started involving hyaline and fibrocartilage at the anterior commissure. Chondroitin‐4‐sulphate was also demonstrated immunohistochemically in the territorial matrix of human nasal septal cartilage (Üstünel et al. 2003a).…”

Section: Discussionmentioning

confidence: 99%

Gender‐specific distribution of glycosaminoglycans during cartilage mineralization of human thyroid cartilage

Claassen

Werner

2004

Journal of Anatomy

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The role of glycosaminoglycans (GAG) in the process of cartilage mineralization, especially in the hypertrophic zone of growth plates, is not yet fully understood. Human thyroid cartilage can serve as a model to observe matrix changes associated with cartilage mineralization because the processes follow a distinct route, progress very slowly and show sexual differences. Histochemical staining for low sulphated GAG (chondroitin-4-and -6-sulphates) was decreased in the interterritorial matrix of thyroid cartilage starting at the beginning of the fifth decade, but not in the pericellular or territorial matrix of chondrocytes. Because cartilage mineralization progressed in the interterritorial matrix it seems likely that a decreasing content of chondroitin-4-and -6-sulphates is involved in the mineralization process. This hypothesis is supported by the observation that immunostaining for chondroitin-4-and -6-sulphates was weaker in mineralized cartilage areas than in unmineralized areas, whereas there was no difference in staining for keratan sulphate. In all life decades, female thyroid cartilages contained more chondrocytes with a territorial rim of chondroitin-4-and -6-sulphates probably preventing cartilage mineralization compared with age-matched male specimens. Taken together, the characteristic distribution pattern of chondroitin-4-and -6-sulphates being more concentrated in female than in male thyroid cartilages provided evidence that these macromolecules decrease in cartilage mineralization.

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Section: Discussionmentioning

confidence: 99%

Gender‐specific distribution of glycosaminoglycans during cartilage mineralization of human thyroid cartilage

Claassen

Werner

2004

Journal of Anatomy

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“…7 The laminin concentration has been found to gradually decrease as one moves from the peripheral to the central parts of the cartilage, while the fibronectin concentration gradually increases, moving from the periphery to the centre. 8 Fibronectin present on cell surfaces has a role in many processes, including cell-cell and cell-substrate adhesion, cell morphology, and cell motility. In the present study, we performed both light and electron microscopic evaluations of the tissue between the perichondrium and the lamina propria, and between the perichondrium and the cartilage matrix.…”

Section: Discussionmentioning

confidence: 99%

“…Their mechanical roles are to provide an intercellular connection, as well as a connection between the different components of the matrix 7 . The laminin concentration has been found to gradually decrease as one moves from the peripheral to the central parts of the cartilage, while the fibronectin concentration gradually increases, moving from the periphery to the centre 8 . Fibronectin present on cell surfaces has a role in many processes, including cell–cell and cell–substrate adhesion, cell morphology, and cell motility.…”

Section: Discussionmentioning

confidence: 99%

Structural characteristics of septal cartilage and mucoperichondrium

et al. 2011

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“…1D) contains both type I collagen and elastin fibers, 20 septal cartilage is similar to articular cartilage in that the major collagen is type II. 21 Adult human septal chondrocytes produce more sGAG and type II collagen in in vitro culture than do human articular 22 or auricular 23 chondrocytes, indicating their superior chondrogenic potential. Both adult human auricular and septal chondrocytes produced more sGAG and expressed higher levels of type II collagen mRNA than did adult human costal chondrocytes in the presence of growth factors in in vitro culture.…”

Section: Differences Among Human Cartilage Tissue Typesmentioning

confidence: 98%

Septal Cartilage Tissue Engineering: New Horizons

Greene

Watson

2010

Facial plast Surg

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Cartilage tissue engineering is a dynamically changing field that has the potential to address some of the tissue repair challenges seen in nasal and craniofacial reconstructive surgeries. The scope of the problem includes limited autologous tissue availability, donor site morbidity associated with the harvesting of these tissue grafts, and the risk of an immune reaction to allogenic or synthetic implants that might be used as alternatives. Current tissue engineering strategies involve harvesting a small biopsy specimen from a patient and then isolating chondrocytes through enzymatic digestion of the extracellular matrix. These isolated chondrocytes can be expanded in monolayer and reseeded into a three-dimensional scaffold that could potentially be used as autologous surgical grafts. Using cell-expansion techniques, it would be feasible to generate abundant amounts of cartilage in defined shapes and sizes. The ideal tissue-engineered cartilage would resemble native tissue in terms of its biochemical, structural, and metabolic properties so that it could restore stability, function, and contour to the damaged or defective facial region. In this article, emerging technology and major challenges are described to highlight recent advances and overall trends within septal cartilage tissue engineering.

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Immunohistochemical distribution patterns of collagen type II, chondroitin 4-sulfate, laminin and fibronectin in human nasal septal cartilage

Cited by 13 publications

References 20 publications

Gender‐specific distribution of glycosaminoglycans during cartilage mineralization of human thyroid cartilage

Gender‐specific distribution of glycosaminoglycans during cartilage mineralization of human thyroid cartilage

Structural characteristics of septal cartilage and mucoperichondrium

Septal Cartilage Tissue Engineering: New Horizons

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