Immunohistochemical distribution of surfactant apoproteins in hypoplastic lungs of nonimmunologic hydrops fetalis

Toki, Naoyuki; Sueishi, Katsuo; Minamitani, Makoto; Maeda, Hitoshi; Nakano, Hitoo; Suzuki, Yuzuru

doi:10.1016/0046-8177(95)90202-3

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…Moreover, surfactant apoprotein B was more diffusely and strongly expressed in type II pneumocytes than surfactant apoprotein A. The previous study of Toki et al (1995) showed SP-A was recognized from 23 gestational weeks of human fetal lung, and diffuse immunoreactivity in alveolar type II cells after 31 gestational weeks. Conversely, SP-B began to be recognized from 20 gestational weeks, and after 26 gestational weeks, most of the cases had diffuse reaction products in alveolar type II cells.…”

Section: Discussionmentioning

confidence: 78%

“…There have been some reports concerning the immunohistochemical results of SP in lung tumors and non-malignant lesions (Dempo et al, 1987;Mizutani et al, 1987;Singh and Katyal, 1980;Toki et al, 1995). According to these studies, the developmental regulation of SP-A and SP-B are known to be different from each other (Toki et al, 1995) and the expression of SPs in cancer cells has been demonstrated in adenocarcinomas, which are considered to be derived from the terminal airways (Dempo et al, 1987;Mizutani et al, 1987). However, there is no report about differential expression of SP-A and SP-B in usual interstitial pneumonia (UIP) associated with pulmonary carcinoma.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical localization of surfactant apoproteins in usual interstitial pneumonia associated with pulmonary carcinoma

Chung

Kitaichi

Ham

et al. 1998

Microsc. Res. Tech.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical localization of surfactant apoproteins in usual interstitial pneumonia associated with pulmonary carcinoma

Chung

Kitaichi

Ham

et al. 1998

Microsc. Res. Tech.

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“…Congenital lymphatic dysplasia may lead to reduced lymph flow, systemic lymphatic reflux, formation of ascites, pleural and pericardial effusions, increased interstitial fluid accumulation and development of nonimmune HF [ 11 ]. Furthermore, HF with excessive accumulation of the fluid in the thoracic cavity may result in pulmonary hypoplasia due to compression [ 12 ] and impaired lung maturation [ 13 ]. Mutations in genes, important for lymphatic development, are known as a possible cause of lymphatic dysplasia in humans.…”

Section: Discussionmentioning

confidence: 99%

Pulmonary hypoplasia and anasarca syndrome in Cika cattle

et al. 2015

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BackgroundHydrops foetalis is defined as excessive fluid accumulation within the foetal extravascular compartments and body cavities. It has been described in human and veterinary medicine, but despite several descriptive studies its aetiology is still not fully clarified. Pulmonary hypoplasia and anasarca (PHA) syndrome is a rare congenital abnormality in cattle that is characterised by hydrops foetalis including extreme subcutaneous oedema (anasarca) and undeveloped or poorly formed lungs (pulmonary hypoplasia). Until now, sporadic cases of PHA were reported in cattle breeds like Australian Dexter, Belted Galloway, Maine-Anjou, and Shorthorn. This report describes the first known cases of PHA syndrome in Slovenian Cika cattle.Case presentationA 13-year-old cow aborted a male calf in the seventh month of pregnancy, while a male calf was delivered by caesarean section on the due date from a 14-year-old cow. The pedigree analysis showed that the calves were sired by the same bull, the dams were paternal half-sisters and the second calf was the product of a dam-son mating. Gross lesions were similar in both cases and characterized by severe anasarca, hydrothorax, hydropericardium, ascites, hypoplastic lungs, absence of lymph nodes, and an enlarged heart. The first calf was also athymic. Histopathology of the second affected calf confirmed severe oedema of the subcutis and interstitium of the organs, and pulmonary hypoplasia. The lymph vessels in the subcutis and other organs were severely dilated. Histopathology of the second calf revealed also lack of bronchus associated lymphoid tissue and adrenal gland hypoplasia.ConclusionsThe findings were consistent with known forms of the bovine PHA syndrome. This is the first report of the PHA syndrome occurring in the local endangered breed of Cika cattle. Observed inbreeding practice supports that this lethal defect most likely follows an autosomal recessive mode of inheritance. In the light of the disease phenotype it is assumed that a mutation causing an impaired development of lymph vessels is responsible for the hydrops foetalis associated malformations in bovine PHA.

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“…SP-A begins to appear in bronchial epithelial cells during the 15th week of gestation, and in alveoli at approximately wk 23 of gestation. Next, at roughly 31 wk of gestation, sufficient amounts of SP-A are expressed in type II alveolar epithelial cells (9).…”

Section: Discussionmentioning

confidence: 99%

Expression of Intrapulmonary Surfactant Apoprotein-A in Autopsied Lungs: Comparative Study of Cases with or without Pulmonary Hypoplasia

et al. 2000

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To investigate the functional maturity of the lungs of infants with pulmonary hypoplasia, we measured the expression of surfactant apoprotein-A (SP-A) in the autopsied lungs. Autopsied lungs were taken from 16 infants who died at birth or soon after. A lung-to-body weight ratio of less than 1.2% was defined as pulmonary hypoplasia. Eight infants were classified as belonging to the normal group, and eight as belonging to the pulmonary hypoplasia group. Many of the pulmonary hypoplasia group were complicated not only by pulmonary hypoplasia, but also by amniotic fluid volume abnormalities or an anatomical malformation. We measured the expression of SP-A immunologically using murine anti-human SP-A MAb in the autopsied lung tissue, and subjected the tissue to SP-A staining by the direct staining method. The expression of SP-A was assessed as one of four grades: Ϫ, Ϯ, 1ϩ, 2ϩ. The staining intensity of SP-A was high at 1ϩ or stronger in five infants of the normal group. SP-A expression was significantly reduced, however, in all infants of the pulmonary hypoplasia group except for one infant with normal amniotic fluid volume and relatively mild pulmonary hypoplasia. There was a significant negative correlation between the staining intensity of SP-A and two factors: pulmonary hypoplasia and abnormal amniotic fluid volume (p ϭ 0.039 and p ϭ 0.0063, respectively). In the present study, we demonstrated that SP-A expression was significantly reduced in infants with pulmonary hypoplasia. We speculate that the functional maturity of the lungs of infants with pulmonary hypoplasia is also suppressed. The alveolar lining is composed of type I and type II epithelial cells. Human pulmonary surfactants are lipid-protein complexes (lipoproteins) synthesized in type II cells. These surfactants are secreted into the alveolar space in order to cover the surface of alveoli and thus reduce alveolar surface tension. The functional expression of pulmonary surfactants requires specific proteins (apoproteins). To date, four types of surfactant apoproteins (SP) have been identified: SP-A, SP-B, SP-C, and SP-D. Of these, SP-A is the most prevalent form of SP (1). SP-A is a hydrophilic glycoprotein with a molecular weight of approximately 28 -36 kD, and the level of SP-A in the amniotic fluid, gastric juice, and respiratory aspirate is a useful indicator of the functional maturity of fetal and neonatal lung tissue (2, 3).In the present study, to assess the functional maturity of the lungs of infants with pulmonary hypoplasia, we measured the expression of SP-A immunohistologically using murine antihuman SP-A MAb in the autopsied lungs of infants who died at birth or soon after. METHODS Subjects.Autopsied lungs were taken from 16 infants at some point during a 21-y period between November 1976 and February 1998. Of the 16 infants, 14 came from the Division of Neonatal Intensive Care or the Department of Gynecology and Obstetrics at our hospital. These subjects had died at birth or within 5 d of birth. The remaining two infants came from the Neo...

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Immunohistochemical distribution of surfactant apoproteins in hypoplastic lungs of nonimmunologic hydrops fetalis

Cited by 6 publications

References 34 publications

Immunohistochemical localization of surfactant apoproteins in usual interstitial pneumonia associated with pulmonary carcinoma

Immunohistochemical localization of surfactant apoproteins in usual interstitial pneumonia associated with pulmonary carcinoma

Pulmonary hypoplasia and anasarca syndrome in Cika cattle

Expression of Intrapulmonary Surfactant Apoprotein-A in Autopsied Lungs: Comparative Study of Cases with or without Pulmonary Hypoplasia

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