Immunohistochemical distribution of glucagon -, insulin -, somatostatin -, gastrin-, and serotonin-containing cells in the pancreas of the Van cat

Karaca, Turan; Kara, Adem; Şimşek, Nejdet; Uslu, Sema; Tekiner, Deniz; Yörük, Mecit

doi:10.3906/vet-1309-72

Cited by 6 publications

(7 citation statements)

References 22 publications

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“…Insulin-, glucagon-, and somatostatin- IR cells were also observed in the exocrine parenchyma, ductus epithelium, and subepithelial connective tissue of the exocrine ducts with lower frequency in this study. Although the presence of endocrine cells in those regions was commonly reported in higher vertebrates, 41,42 similar observations were reported in some fish species. Immunoreactive cells were detected among the exocrine acini; however, no immunoreactivity was noted in the ducts of the exocrine pancreas of the five species of osteoglossomorha.…”

Section: Discussionsupporting

confidence: 83%

Immunohistochemical distribution of insulin-, glucagon- and somatostatin-containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae)

Kaptaner

2019

Eur J Histochem

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The Lake Van fish (Alburnus tarichi) is a species that is endemic to Turkey’s Lake Van basin. In this study, the regional distribution, volume density, and relative frequency of some pancreatic endocrine cells in Lake Van fish were investigated via immunohistochemistry using specific mammalian antibodies. The pancreatic tissue was observed to be surrounded by adipose tissue, which was adjacent to the gall bladder or extrahepatic bile duct, or dispersed in the adipose tissue ranked among coils of post-esophageal swelling and intestine. The pancreatic endocrine cells were examined, including the islets, exocrine pancreas, and pancreatic ducts. According to the modified aldehyde fuchsin staining and immunohistochemistry, insulin-secreting beta cells were observed to localize throughout the islets. Glucagon immune-reactive (IR) cells were observed to be situated moderately on the islet periphery, and were rarely determined in the islet central region. A small number of somatostatin-IR cells were observed in the islet centers and peripheries. Similar distributions of those 3 endocrine cells were also determined in the secondary islets. Additionally, the endocrine cell percentages did not differ between the primary and secondary islets; insulin-, glucagon- and somatostatin-IR cells comprised approximately 54%, 29%, and 11% of the endocrine cells in the principal islets, whereas they comprised 52%, 27%, and 14% in the secondary islets, respectively. Insulin-, glucagon- and somatostatin-IR cells were also determined among the epithelium and subepithelial connective tissue in the pancreatic ducts or exocrine areas of the pancreas. With this study, the existence, regional distribution, and relative frequency of the insulin-, glucagon- and somatostatin-IR cells were first investigated in the pancreatic tissue of Lake Van fish and the results were discussed.

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Section: Discussionsupporting

confidence: 83%

Immunohistochemical distribution of insulin-, glucagon- and somatostatin-containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae)

Kaptaner

2019

Eur J Histochem

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“…Ahmed (22) (27) in cat recorded that β-cells occupied the center of the islet whereas α-cells were located at periphery. Differently, other investigators like Dhoolappa (28) in Indian donkey and Karaca et al (29) in Van cat documented that α-cells were tend to occur in the center of the islet and β-cells towards the periphery. Tsuchitani et al (30) in minipig mentioned that both α-and β-cells were present in all parts of the islet, intermingling with each other.…”

Section: Discussionmentioning

confidence: 72%

Histological and ultrastructural studies of the pancreas of native rabbits (Oryctolagus cuniculus)

Mohammed¹

2019

IJVS

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The pancreas of male adult native rabbit was studied by using light microscope (LM) and transmission electron microscope (TEM). The pancreas possessed both exocrine and endocrine portions. Exocrine portion included both acinar and ductal cells. The acinar cells were pyramidal in shaped which formed the lining epithelia of secretory units (acini). The apical cytoplasm of acinar cells contained unequal size and variable electron opacity of zymogen granules, while the basal part occupied by welldeveloped rough endoplasm reticulum (rER). Short microvilli projected from the free surfaces of both acinar and ductal cells. The lateral cell membranes of adjacent acinar and ductal cells adhere apically by junctional complex, which was composed of zonulae occludens and zonulae adherens. There was notable interdigitating between the lateral cell membranes of neighboring ductal cells that were located beneath the junctional complex. The oval or irregular pancreatic islets consisted of β, α and δ endocrine cells. Both β-and α-cells could be demonstrated by using LM and TEM, whereas δ-cells visualized with TEM only. The cytoplasm of β-cells contained purple granules and α-cells showed yellowish granules in their cytoplasm when stained with aldehyde fuchsine method. At ultrastructure level, the cytoplasmic granules of β-cells were identified by homogenous electron dense core with clear hallo zone, the numerous granules of α-cells had very electron dense granules and δ-cells contained few granules similar to those of α-cells.β-or α-cells inserted as a single cell and in a partial manner between the ductal cells of some ducts.

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“…In addition, individual endocrine cells such as α, β, and δ cells can be observed outside the islets (Figures 6-8). This phenomenon was also detected in and many other mammalian species, including rat, cat, dog, pig, non-human primate, bottlenose dolphin, and beluga whale (24,26,34,35). Although why this kind of endocrine cell arrangements in these species are still largely unknown, some studies suggested that these alone endocrine cells may be an indication of proliferation or neogenesis (34)(35)(36)(37).…”

Section: Discussionmentioning

confidence: 82%

Characterization of Insulin and Glucagon Genes and Their Producing Endocrine Cells From Pygmy Sperm Whale (Kogia breviceps)

et al. 2020

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Insulin and glucagon are hormones secreted by pancreatic β and α cells, respectively, which together regulate glucose homeostasis. Dysregulation of insulin or glucagon can result in loss of blood glucose control, characterized by hyperglycemia or hypoglycemia. To better understand the endocrine physiology of cetaceans, we cloned and characterized the insulin and glucagon genes from pygmy sperm whale (Kogia breviceps). We obtained the complete coding sequences of the preproinsulin and preproglucagon genes, which encodes the preproinsulin protein of 110 amino acid (aa) residues and encodes the preproglucagon protein of 179 aa residues, respectively. Sequence comparison and phylogenetic analyses demonstrate that protein structures were similar to other mammalian orthologs. Immunohistochemistry and immunofluorescence staining using insulin, glucagon, and somatostatin antibodies allowed analysis of pygmy sperm whale islet distribution, architecture, and composition. Our results showed the pygmy sperm whale islet was irregularly shaped and randomly distributed throughout the pancreas. The architecture of α, β, and δ cells of the pygmy sperm whale was similar to that of artiodactyls species. This is the first report about insulin and glucagon genes in cetaceans, which provides new information about the structural conservation of the insulin and glucagon genes. Furthermore, offers novel information on the properties of endocrine cells in cetacean for further studies.

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Immunohistochemical distribution of glucagon -, insulin -, somatostatin -, gastrin-, and serotonin-containing cells in the pancreas of the Van cat

Cited by 6 publications

References 22 publications

Immunohistochemical distribution of insulin-, glucagon- and somatostatin-containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae)

Immunohistochemical distribution of insulin-, glucagon- and somatostatin-containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae)

Histological and ultrastructural studies of the pancreas of native rabbits (Oryctolagus cuniculus)

Characterization of Insulin and Glucagon Genes and Their Producing Endocrine Cells From Pygmy Sperm Whale (Kogia breviceps)

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