Immunohistochemical distribution of calretinin and calbindin (D‐28k) in the brain of the cladistian Polypterus senegalus

Graña, Patricia; Folgueira, Mónica; Huesa, Gema; Anadón, Ramón; Yáñez, Julián

doi:10.1002/cne.23293

Cited by 18 publications

(59 citation statements)

References 140 publications

(246 reference statements)

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“…The antiserum has previously been used in several nonmammalian species, including different fishes and amphibians, and it has shown no cross-reaction with other monoamines [Stuesse and Cruce, 1992;Marín et al, 1998;Adrio et al, 1999;Antri et al, 2006;Abalo et al, 2007;Carrera et al, 2008;López et al, 2009;. The specificity of ChAT, CB, and CR antisera has been previously described in Polypterus [Graña et al, 2013;, and the distribution patterns for these markers are similar in the brain of Erpetoichthys (present results).…”

mentioning

confidence: 61%

“…1 h, 4 e). A remarkable population of CBcontaining cells has been observed in the PVO, described within the dorsal and caudal hypothalamic parts [Graña et al, 2013] with CSF-contacting processes, and we observed that they intermingled with the serotonergic cells, but no double-labeled cells were detected.…”

Section: Forebrainmentioning

confidence: 71%

“…To correlate the serotonergic structures with the neurons containing CB and CR, and with the cholinergic cell groups, of which the precise localization in Polypterus was recently established [Graña et al, 2013;, a procedure based on double immunohistofluorescence was used as follows: (1) a first incubation for 72 h at 4 ° C in a mixture of rabbit anti-5-HT (diluted 1: 1,000) and goat anti-ChAT (diluted 1: 100, AB144P; Chemicon, Temecula, Calif., USA), mouse anti-CB (diluted 1: 1,000, catalog No. 300; Swant, Bellizona, Switzerland), or mouse anti-CR (diluted 1: 1,000, catalog No.…”

Section: Double 5-ht and Chat Cb Or Cr Immunolabelingmentioning

confidence: 99%

“…A few studies of Polypterus have reported some hodological features, mainly related to the olfactory system and telencephalon [Braford and Northcutt, 1974;Northcutt, 1981Northcutt, , 2004von Bartheld and Meyer, 1986;Holmes and Northcutt, 2003]. There are even fewer immunohistochemical studies addressing the neurochemical organization of the brain in Polypterus [Reiner and Northcutt, 1992;Wright and Demski, 1996;Chiba, 1997;Graña et al, 2013;.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we will compare our results with those obtained in other vertebrate classes to gain further insight into the phylogenetic evolution of this neurotransmitter system. In addition, we combined immunohistochemistry for the detection of 5-HT with that for the detection of choline acetyltransferase (ChAT), and the calcium-binding proteins calbindin-D28k (CB) and calretinin (CR), because the neuroanatomy of cladistians is not well documented, and the combined localization of these markers, the distribution patterns of which have been recently described [Graña et al, 2013;, is useful in clarifying the actual position of certain cell groups.…”

Section: Introductionmentioning

confidence: 99%

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Organization of the Serotonergic System in the Central Nervous System of Two Basal Actinopterygian Fishes: the Cladistians Polypterus senegalus and Erpetoichthys calabaricus

López¹,

González²

2014

Brain Behav Evol

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Cladistians (Polypteriformes) are currently considered basal to other living ray-finned fishes (actinopterygians), and their brain organization is therefore critical to providing information about the primitive neural characters that existed in the earliest ray-finned fishes. The organization of the serotonergic system in the brain has been carefully analyzed in most vertebrate groups, and in the present study we provide the first detailed information on the distribution of serotonergic cell bodies and fibers in the central nervous system of representative species of the two extant genera of cladistians, i.e. Polypterus senegalus and Erpetoichthys calabaricus, by means of immunohistochemistry against serotonin (5-HT). Distinct groups of immunoreactive cells were detected in the preoptic area, the hypothalamic paraventricular organ, the pineal organ, the pretectal region, the long column of the raphe in the rhombencephalic midline, the spinal cord, and amacrine cells in the inner nuclear layer of the retina. Fiber labeling was widely distributed in all main brain subdivisions but was more abundant in distinct pallial and subpallial areas, the preoptic area, the thalamus, the optic tectum, the tori semicircularis and lateralis, the rhombencephalic reticular formation, the nucleus of the solitary tract, and the dorsal aspect of the spinal cord. Our analysis makes it possible to establish which serotonergic structures characterized the earliest ray-finned fishes, and a comparison of these results with those from other classes of vertebrates, including a segmental analysis to correlate cell populations, reveals that most characteristics, such as the presence of serotonergic cells in the preoptic area and the basal hypothalamus, are preserved in all anamniotes. However, this system seems to be reduced in amniotes, mainly mammals, although important features are shared, such as the presence of serotonergic cells in the pineal organ, the retina, and the raphe nuclei.

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mentioning

confidence: 61%

Section: Forebrainmentioning

confidence: 71%

Section: Double 5-ht and Chat Cb Or Cr Immunolabelingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Organization of the Serotonergic System in the Central Nervous System of Two Basal Actinopterygian Fishes: the Cladistians Polypterus senegalus and Erpetoichthys calabaricus

López¹,

González²

2014

Brain Behav Evol

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Neuronal organization of the brain in the adult amphioxus (Branchiostoma lanceolatum): A study with acetylated tubulin immunohistochemistry

Castro

Becerra

Manso

et al. 2015

J of Comparative Neurology

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Amphioxus (Cephalochordata) belongs to the most basal extant chordates, and knowledge of their brain organization appears to be key to deciphering the early stages of evolution of vertebrate brains. Most comprehensive studies of the organization of the central nervous system of adult amphioxus have investigated the spinal cord. Some brain populations have been characterized via neurochemistry and electron microscopy, and the overall cytoarchitecture of the brain was studied by Ekhart et al. (2003; J. Comp. Neurol. 466:319-330) with general staining methods and retrograde transport from the spinal cord. Here, the cytoarchitecture of the brain of adult amphioxus Branchiostoma lanceolatum was reinvestigated by using acetylated tubulin immunohistochemistry, which specifically stains neurons and fibers, in combination with some ancillary methods. This method allowed reproducible staining and mapping of types of neuron, mostly in brain regions caudal to the entrance level of nerve 2, and its comparison with spinal cord populations. The brain populations studied and discussed in detail were the Retzius bipolar cells, lamellate cells, Joseph cells, various types of translumenal cells, somatic motoneurons, Rohde nucleus cells, small ventral multipolar neurons, and Edinger cells. These observations expand our knowledge of the distribution of cell types and provide additional data on the number of cells and the axonal tracts and commissural regions of the adult amphioxus brain. The results of this comprehensive study provide a framework for comparison of complex adult populations with the early brain neuronal populations revealed in developmental studies of the amphioxus.

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Organization of the nitrergic neuronal system in the primitive bony fishesPolypterus senegalusandErpetoichthys calabaricus(Actinopterygii: Cladistia)

López

Lozano

Morona

et al. 2015

J of Comparative Neurology

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Cladistians are a group of basal actinopterygian fishes that constitute a good model for studying primitive brain features, most likely present in the ancestral bony fishes. The analysis of the nitrergic neurons (with the enzyme nitric oxide synthase; NOS) has helped in understanding important aspects of brain organization in all vertebrates studied. We investigated the nitrergic system of two cladistian species by means of specific antibodies against NOS and NADPH-diaphorase (NADPH-d) histochemistry, which, with the exception of the primary olfactory and terminal nerve fibers, labeled only for NADPH-d, yielded identical results. Double immunohistochemistry was conducted for simultaneous detection of NOS with tyrosine hydroxylase, choline acetyltransferase, calbindin, calretinin, and serotonin, to establish accurately the localization of the nitrergic neurons and fibers and to assess possible interactions between these neuroactive substances. The pattern of distribution in both species showed only subtle differences in the density of labeled cells. Distinct groups of NOS-immunoreactive cells were observed in pallial and subpallial areas, paraventricular region, tuberal and retromammillary hypothalamic areas, posterior tubercle, prethalamic and thalamic areas, optic tectum, torus semicircularis, mesencephalic tegmentum, interpeduncular nucleus, superior and middle reticular nuclei, magnocellular vestibular nucleus, solitary tract nucleus, nucleus medianus magnocellularis, the spinal cord and amacrine cells in the retina. Large neurons in cranial nerve sensory ganglia were also labeled. The comparison of these results with those from other vertebrates, using a neuromeric analysis, reveals a conserved pattern of organization of the nitrergic system from this primitive fish group to amniotes, including mammals.

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Immunohistochemical distribution of calretinin and calbindin (D‐28k) in the brain of the cladistian Polypterus senegalus

Cited by 18 publications

References 140 publications

Organization of the Serotonergic System in the Central Nervous System of Two Basal Actinopterygian Fishes: the Cladistians <b><i>Polypterus senegalus</i></b> and <b><i>Erpetoichthys calabaricus</i></b>

Organization of the Serotonergic System in the Central Nervous System of Two Basal Actinopterygian Fishes: the Cladistians <b><i>Polypterus senegalus</i></b> and <b><i>Erpetoichthys calabaricus</i></b>

Neuronal organization of the brain in the adult amphioxus (Branchiostoma lanceolatum): A study with acetylated tubulin immunohistochemistry

Organization of the nitrergic neuronal system in the primitive bony fishesPolypterus senegalusandErpetoichthys calabaricus(Actinopterygii: Cladistia)

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