Immunohistochemical detection of transforming growth factor-α, epidermal growth factor, and vascular endothelial growth factor expression in hyperstimulated rat ovary

Özçakır, Hasan Tayfun; Giray, Seren Gülşen; Özbilgin, Mahmut Kemal; Uyar, Yýldýz; Laçin, Selman; Çağlar, Hüsnü

doi:10.1080/j.0001-6349.2005.00586.x

Cited by 7 publications

(6 citation statements)

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“…Ozcakır et al [18] reported that VEGF immunoreactivity is stronger in the endothelium of blood vessels, the corpus luteum, and the stroma in OHSS groups compared with control groups. In line with the findings of this study, the present authors also observed VEGF-stained cell counts were higher in the OHSS placebo group than in the control group.…”

Section: Discussionmentioning

confidence: 99%

“…Other than VEGF, prostaglandins, cytokines, the renin-angiotensin-aldosterone system, estradiol, progesterone, the kinin-kallikrein system, and nitric oxide have been implicated in the etiology of OHSS [16,17]. There are studies in literature in which serum and peritoneal fluid VEGF levels have been shown to be reduced by the above mentioned pathways, and these effects prevented the development of OHSS [18][19][20]. Furthermore, Yilmaz et al [21] showed that FF reduces VEGF levels in the peritoneal fluid of animals with induced endometriosis.…”

Section: Discussionmentioning

confidence: 99%

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The effects of formoterol on the serum, peritoneal VEGF, MDA, and VEGF levels in the ovaries and endometrium of rats with OHSS

İnal¹,

Inal²,

Yılmaz³

et al. 2017

Clin. Exp. Obstet. Gynecol.

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To investigate the effects of formoterol (a beta2-adrenoreceptor agonist) on serum and peritoneal fluid VEGF, malondialdehyde (MDA) levels, and on VEGF-stained cell counts in the ovaries and endometrium of rats with ovarian hyperstimulation syndrome (OHSS) within the framework of immunohistochemical analysis. Materials and Methods: A total of 28 immature female Wistar rats were randomly divided into four groups. Three groups were given ten IU pregnant mare serum gonadotropin/day on days 22-25 of life. They were administered 30 IU hCG on day 26 of life to mimic OHSS. On days 26 and 27 of life, 24 mcg/kg/day formoterol in group 3 and 48 mcg/kg formoterol in group 4 were administered intraperitoneally per animal. Results: Although, there were no statistically significant differences between the groups in terms of serum and peritoneal fluid VEGF or MDA levels (serum VEGF: p = 0.281, peritoneal VEGF: p = 0.674, serum MDA: p = 0.543, peritoneal MDA: p = 0.506), there was a significant difference between the control and the OHSS placebo groups (p = 0.013) regarding the VEGF in the ovarian cortex. There was a significant difference between the control and the other groups in terms of ovarian stroma (p = 0.001), and there was also a statistically significant difference between the OHSS placebo and the other groups regarding VEGF in the endometrium (OHSS placebo vs. control group p = 0.002, OHSS placebo vs. the formoterol 24 mcg/kg group, p = 0.008, and OHSS-placebo vs. the formoterol 48 mcg/kg group, p = 0.001). Conclusions: Formoterol represents a potential novel strategy for the management of OHSS. Further studies, including those examining the dosage of formoterol, are warranted.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The effects of formoterol on the serum, peritoneal VEGF, MDA, and VEGF levels in the ovaries and endometrium of rats with OHSS

İnal¹,

Inal²,

Yılmaz³

et al. 2017

Clin. Exp. Obstet. Gynecol.

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“…TGF-α acts to promote the proliferation and differentiation of germ cells. In vitro studies have exhibited that TGF-α is expressed in ovarian follicles and theca-interstitial cells and regulates follicular growth, degeneration and atresia [29]. In the primordial phase, TGF-α is highly expressed in the oocytes, and as the primordial follicles develop, its expression decreases, indicating that it mainly modulates the development of primordial follicles and oocytes [30].…”

Section: Discussionmentioning

confidence: 99%

Clinical characteristics and risk factors of ovarian reserve decreases in women with Crohn’s disease: a case-control study

Xiang

Yao

et al. 2023

J Ovarian Res

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Background Crohn’s disease (CD), often occurring in women of child-bearing age, can decline the fertility rate. However, whether it reduces ovarian reserve has been rarely reported. This study aimed to evaluate the ovarian reserve in women with CD from the perspective of anti-Müllerian hormone (AMH), and explore the factors that can decrease ovarian reserve. Methods A case-control retrospective study was designed. We analyzed the AMH levels in a total of 135 CD women and 878 healthy controls. Through propensity score matching, the subjects were assigned in a ratio of 1:3 to CD group (n = 121) and control group (n = 324). Both groups shared similar basic characteristics, like age, body mass index and smoking status. Serum AMH levels were measured by chemiluminescence. Results The AMH level in the CD group was significantly lower than that in the control group (2.17 ± 2.23 μg/L vs 3.95 ± 2.01 μg/L, 95%CI [1.34-2.21], P < 0.001). In both groups, the AMH levels decreased as age increased, but without between-group difference in the decreasing rate (P = 0.639). Multivariate analysis showed that age > 30 years (OR, 2.905; 95%CI [1.053-8.531], P = 0.017), disease activity (OR,4.314; 95%CI [1.561-12.910], P = 0.002) and thalidomide use (OR,12.628; 95%CI [4.351 -42.820], P < 0.001) were independent risk factors associated with decreased ovarian reserve (AMH<1.1μg/L). Conclusion Ovarian reserve is lower in CD women than in healthy women. Age, CD activity and medication of thalidomide are risk factors that can aggravate the decline of ovarian reserve.

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“…There are few studies in the world which try to find a relationship between VEGF gene polymorphism and OHSS (4-7). Two of the polymorphisms -460 C/T and +405 G/C were reported to be associated with increased VEGF basal promoter activity.…”

Section: Discussionmentioning

confidence: 99%

“…Vasoactive properties and increased ovarian expression during the development of OHSS suggest that VEGF plays a major role in the development of this syndrome. Serum VEGF levels increase after Human chorionic gonadotropin (hCG) administration in superovulated women, which could be a risk of development OHSS (5-7). In women who developed OHSS, VEGF was released into the follicular fluid in response to hCG, which increased capillary permeability (8).…”

Section: Introductionmentioning

confidence: 99%

Association of -460C/T and +405 G/C polymorphisms of vascular endothelial growth factor gene and susceptibility of ovarian hyperstimulation syndrome

Ghasemi

Firouzabadi

Ahmadi

2017

IJRM

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Background:Ovarian hyperstimulation syndrome (OHSS) is one of the most important complications of assisted reproduction treatment. Many substances are involved in the regulation of the vascular permeability, which have been concerned to cause OHSS. Vascular endothelial growth factor (VEGF) has emerged as one of the main angiogenic factors, which could be responsible for increased vascular permeability.Objective: In this study the association of vascular endothelial growth factor -460C/T and +405 G/C polymorphisms and susceptibility to ovarian hyperstimulation syndrome was evaluated. Materials and Methods:In this cross sectional study, VEGF gene polymorphisms were amplified by Polymerase chain reaction- Restriction Fragment Length Polymorphism in 75 women with established OHSS (case group) and 85 normoresponder (control group) which received conventional ovarian stimulation regimen. Results:There was no significant difference in the frequency of -460 C/T polymorphism between cases and controls (p=0.85). The frequency of +405 G/C polymorphism was significantly higher in the OHSS women (p=0.03, OR=2.44; 95% CI=1.23-4.82). Conclusion: In women who developed OHSS, VEGF gene polymorphism +405 could be effective. Two of the polymorphisms -460 C/T and +405 G/C were reported to be associated with increased VEGF basal promoter activity. However, only +405 G/C gene polymorphisms were more frequent in cases than controls.

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Immunohistochemical detection of transforming growth factor-α, epidermal growth factor, and vascular endothelial growth factor expression in hyperstimulated rat ovary

Abstract: TGF-alpha, EGF, and VEGF are found to have increased in the hyperstimulated ovaries and this finding seems to be involved in the OHSS pathogenesis.

Cited by 7 publications

References 0 publications

The effects of formoterol on the serum, peritoneal VEGF, MDA, and VEGF levels in the ovaries and endometrium of rats with OHSS

The effects of formoterol on the serum, peritoneal VEGF, MDA, and VEGF levels in the ovaries and endometrium of rats with OHSS

Clinical characteristics and risk factors of ovarian reserve decreases in women with Crohn’s disease: a case-control study

Association of -460C/T and +405 G/C polymorphisms of vascular endothelial growth factor gene and susceptibility of ovarian hyperstimulation syndrome

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