Immunohistochemical detection of P-glycoprotein and GSTP1-1 in testis cancer

Katagiri, Atsuko; Tomita, Y; Nishiyama, T; Kimura, Masahiro; Sato, Shigeaki

doi:10.1038/bjc.1993.299

Cited by 40 publications

(18 citation statements)

References 21 publications

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“…Pgp expression was observed in 27% of tumours, in agreement with previous reports (35%, Katagiri et al, 1993;33%, Eid et al, 1998). In contrast to the report by Eid et al (1998), we found no association between Pgp expression and nonseminomatous histology or advanced stages of disease.…”

Section: Discussionsupporting

confidence: 82%

Lung resistance-related protein as a predictor of clinical outcome in advanced testicular germ-cell tumours

et al. 2003

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This study was undertaken to investigate the expression and predictive value for outcome of multidrug resistance-associated (MDR) proteins P-glycoprotein (Pgp), MRP1, BCRP, and LRP, in advanced testicular germ-cell tumours (TGCT). Paraffin-embedded sections from 56 previously untreated patients with metastatic TGCT were immunostained for Pgp, MRP1, BCRP, and LRP. All patients received platinum-based chemotherapy after orchidectomy. Immunostaining was related to clinicopathological parameters, response to chemotherapy, and outcome. Strong and intermediate expressions of the different MDR-related proteins were: 27 and 41% (Pgp), 54 and 37% (MRP1), 86 and 7% (BCRP), and 14 and 29% (LRP). P-glycoprotein and MRP1 associated, respectively, to low AFP (P ¼ 0.026) and high LDH levels (P ¼ 0.014), whereas LRP expression associated with high b-hCG levels (P ¼ 0.003) and stage IV tumours (P ¼ 0.029). No correlation was found between Pgp, MRP1, and BCRP expression and response to chemotherapy and survival. In contrast, patients with LRP-positive tumours (strong or intermediate expression) had shorter progression-free (P ¼ 0.0006) and overall survival (P ¼ 0.0116) than LRP-negative patients, even after individual log-rank adjustments by statistically associated variables. Our data suggest that a positive LRP immunostaining at the time of diagnosis in metastatic TGCT is associated with an adverse clinical outcome.

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Section: Discussionsupporting

confidence: 82%

Lung resistance-related protein as a predictor of clinical outcome in advanced testicular germ-cell tumours

et al. 2003

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“…Wc have recently shown that MAb C494 crossreacts with pyruvate carboxylase (PC), and a search for epitopespecific sequences of MAb C494 revealed that mouse PC contains four of the five most reactive amino acids (REG) near the carboxy terminus (17). Although the epitope of MAb JSB-1 has not been characterized, it is being widely used for immunohistochemical detection of P-gp in clinical tissues specimens (18,19). We show here that the MAb JSB-1 also crossreacts with PC under both immunoblotting and immunohistochemical conditions.…”

Section: Introductionmentioning

confidence: 73%

“…In summary, MAb JSB-1 is known for excellent immunostaining of P-gp in formalin-fixed, paraffin-embedded, and frozen tissue sections (34), and is widely used for detection of MDRl P-gp expression in clinical specimens (18,19,35). The present study shows intense immunohistochemical staining with MAb JSB-1 in a tissue lacking expression of MDRl P-gp and has identified at least one crossreactive polypeptide as PC, an enzyme abundant in mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Multidrug resistance P-glycoprotein monoclonal antibody JSB-1 crossreacts with pyruvate carboxylase.

Rao

Anthony

Piwnica-Worms³

1995

J Histochem Cytochem.

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Multidrug resistance (MDR) is associated with overexpression of a 170 KD plasma membrane P-glycoprotein (P-gp), a putative energy-dependent efflux transporter that reduces intracellular accumulation of chemotherapeutic agents. For detection of P-gp expression in normal and malignant tissues, an MDR1-specific monoclonal antibody (MAb) JSB-1 has been used extensively. In this report we show that MAb JSB-1 crossreacts with a protein of M(r) approximately 130,000 present in rat liver mitochondrial inner membrane/matrix fractions. Peptide mapping and microsequencing identify this protein as pyruvate carboxylase (PC), an abundant mitochondrial enzyme. MAb JSB-1 also crossreacts with purified PC from bovine liver. Under immunoblotting conditions, this crossreactivity is partially abolished by pre-incubation of MAb JSB-1 with a 1000-fold molar excess of MAb C494 epitope-specific peptide (PNTLEGN), indicating that the epitope of MAb JSB-1 may either overlap with or be in close proximity to that of MAb C494. Immunohistochemical cross-reactivity was also demonstrated in cryosections of human skeletal muscle, a tissue known not to express P-gp. MAb JSB-1 strongly immunostained Type 1 fibers, the subtype known to contain abundant mitochondria. Use of MAb JSB-1 for detection of MDR1 P-gp expression should be approached with caution.

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“…The high expression of GST-pi in many neoplasms was related to the resistance of anticancer drugs. Katagiri et al reported that the expression of GST-pi negatively correlated to the sens etivity of anticancer drug in testicular tumor [6] . Dong J suggested that the expression of GST-pi closely related to resistance of anticancer drug in ovariogenic neoplasm [7] .…”

Section: Biological Characters Of Gst-pimentioning

confidence: 99%

Ultrastructural localization of glutathione S-transferase-pi in human colorectal cancer cells

Guo

2000

WJG

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Immunohistochemical detection of P-glycoprotein and GSTP1-1 in testis cancer

Cited by 40 publications

References 21 publications

Lung resistance-related protein as a predictor of clinical outcome in advanced testicular germ-cell tumours

Lung resistance-related protein as a predictor of clinical outcome in advanced testicular germ-cell tumours

Multidrug resistance P-glycoprotein monoclonal antibody JSB-1 crossreacts with pyruvate carboxylase.

Ultrastructural localization of glutathione S-transferase-pi in human colorectal cancer cells

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