Immunohistochemical detection of neurotrophin-3 and -4, and their receptors in mouse taste bud cells

Takeda, Masako; Obara, Naoshi; Tsunekawa, Hiroaki

doi:10.1679/aohc.68.393

Cited by 10 publications

(7 citation statements)

References 47 publications

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“…For instance, glial cell line-derived neurotrophic factor is expressed in a different subset of taste bud cells than BDNF (Suzuki et al, 2007; Takeda et al, 2004), and its receptors are expressed in the geniculate ganglion (Farbman et al, 2004b; Yamout et al, 2005). However, many other factors could also play this role/s (Feng et al, 2014; Germana et al, 2006; McLaughlin, 2000; Suzuki et al, 2007; Suzuki et al, 2005; Takeda et al, 2005; Yee et al, 2005). …”

Section: Discussionmentioning

confidence: 99%

Taste bud-derived BDNF maintains innervation of a subset of TrkB-expressing gustatory nerve fibers

Tang

Rios-Pilier

Krimm

2017

Molecular and Cellular Neuroscience

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Taste receptor cells transduce different types of taste stimuli and transmit this information to gustatory neurons that carry it to the brain. Taste receptor cells turn over continuously in adulthood, requiring constant new innervation from nerve fibers. Therefore, the maintenance of innervation to taste buds is an active process mediated by many factors, including brain-derived neurotrophic factor (BDNF). Specifically, 40% of taste bud innervation is lost when Bdnf is removed during adulthood. Here we speculated that not all gustatory nerve fibers express the BDNF receptor, TrkB, resulting in subsets of neurons that vary in their response to BDNF. However, it is also possible that the partial loss of innervation occurred because the Bdnf gene was not effectively removed. To test these possibilities, we first determined that not all gustatory nerve fibers express the TrkB receptor in adult mice. We then verified the efficiency of Bdnf removal specifically in taste buds of K14-CreER:Bdnf mice and found that Bdnf expression was reduced to 1%, indicating efficient Bdnf gene recombination. BDNF removal resulted in a 55% loss of TrkB-expressing nerve fibers, which was greater than the loss of P2X3-positive fibers (39%), likely because taste buds were innervated by P2X3+/TrkB− fibers that were unaffected by BDNF removal. We conclude that gustatory innervation consists of both TrkB-positive and TrkB-negative taste fibers and that BDNF is specifically important for maintaining TrkB-positive innervation to taste buds. In addition, although taste bud size was not affected by inducible Bdnf removal, the expression of the γ subunit of the ENaC channel was reduced. So, BDNF may regulate expression of some molecular components of taste transduction pathways.

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Section: Discussionmentioning

confidence: 99%

Taste bud-derived BDNF maintains innervation of a subset of TrkB-expressing gustatory nerve fibers

Tang

Rios-Pilier

Krimm

2017

Molecular and Cellular Neuroscience

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“…This theory is supported by findings that the trkB and p75 receptors are widely expressed throughout the oral cavity and gastrointestinal tract. For example, immunoreactivity for trkB is expressed in all three types of mouse taste cells37, while p75 is found in Type II and III taste cells 38. p75 has also been also detected in the intercalated ducts of human sublingual gland tissue and the ductal and acinar cells of the submandibular gland in mice 39.…”

Section: Discussionmentioning

confidence: 99%

Identification of pro- and mature brain-derived neurotrophic factor in human saliva

Mandel

Ozdener

Utermohlen

2009

Archives of Oral Biology

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Objective-Growth factors, including brain-derived neurotrophic factor (BDNF), are polypeptides that are involved in the maintenance, survival, and death of central and peripheral cells. Numerous growth factors have been identified in saliva and are thought to promote wound healing and maintenance of the oral epithelium. The aim of this study was to determine if BDNF is also found in human saliva.Methods-Whole, unstimulated saliva samples (n=30) were analyzed by SDS-PAGE and Western blot using an anti-human BDNF antibody. Proteolytic cleavage products were similarly assessed following the incubation of pooled saliva with N-glycanase F and plasmin. Subjects genotyped for the BDNF Val66Met single nucleotide polymorphism (SNP).Results-These experiments revealed the presence of immunoreactive bands at 14, 32 and 34 kD, corresponding to mature (mBDNF) and proBDNF, as well as a truncated pro-form at 24 kD. Not every sample contained all forms of BDNF. Treatment with N-glycanase and plasmin reduced the size of the higher molecular weight bands, confirming the glycosylated pro-form of BDNF. mBDNF was detected significantly less often in subjects with the Val66Met SNP, compared to those without the polymorphism (X 2 = 4.05; P<0.05).Conclusions-While the function of salivary BDNF still requires elucidation, these findings suggest that it may be possible to use saliva in lieu of blood in future studies of BDNF and the Val66Met polymorphism.

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“…Both BDNF and TrkB are present in developing and adult taste buds (Nosrat and Olson 1995;Nosrat et al 1996;Nosrat 1998;Takeda et al 2005). This raises the possibility of BDNF being secreted by taste cells and TrkB activation of taste cells in a paracrine/autocrine manner.…”

Section: Discussionmentioning

confidence: 99%

Gustatory papillae and taste bud development and maintenance in the absence of TrkB ligands BDNF and NT-4

Ito

Nosrat

2009

Cell Tissue Res

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Taste buds and the peripheral nerves innervating them are two important components of the peripheral gustatory system. They require appropriate connections for the taste system to function. Neurotrophic factors play crucial roles in the innervation of peripheral sensory organs and tissues. Both brain-derived neurotrophic factor (BDNF) null-mutated and neurotrophin-4 (NT-4) null-mutated mice exhibit peripheral gustatory deficits. BDNF and NT-4 bind to a common high affinity tyrosine kinase receptor, TrkB (NTRK-2), and a common p75 neurotrophin receptor (NGFR). We are currently using a transgenic mouse model to study peripheral taste system development and innervation in the absence of both TrkB ligands. We show that taste cell progenitors express taste cell markers during early stages of taste bud development in both BDNF(-/-)xNT-4(-/-) and wild-type mice. At early embryonic stages, taste bud progenitors express Troma-1, Shh, and Sox2 in all mice. At later stages, lack of innervation becomes a prominent feature in BDNF(-/-)xNT-4(-/-) mice leading to a decreasing number of fungiform papillae and morphologically degenerating taste cells. A total loss of vallate taste cells also occurs in postnatal transgenic mice. Our data indicate an initial independence but a later permissive and essential role for innervation in taste bud development and maintenance.

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Immunohistochemical detection of neurotrophin-3 and -4, and their receptors in mouse taste bud cells

Cited by 10 publications

References 47 publications

Taste bud-derived BDNF maintains innervation of a subset of TrkB-expressing gustatory nerve fibers

Taste bud-derived BDNF maintains innervation of a subset of TrkB-expressing gustatory nerve fibers

Identification of pro- and mature brain-derived neurotrophic factor in human saliva

Gustatory papillae and taste bud development and maintenance in the absence of TrkB ligands BDNF and NT-4

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