Immunohistochemical detection of lipid peroxidation products, protein-bound acrolein and 4-hydroxynonenal protein adducts, in actinic elastosis of photodamaged skin

Abstract

Acrolein and 4-hydroxy-2-nonenal (HNE) are both byproducts of a lipid peroxidation reaction. Actinic elastosis in photodamaged skin of aged individuals is characterized by the accumulation of fragmented elastic fibers in the sun-exposed areas. To study whether a lipid peroxidation reaction is involved in the accumulation of altered elastic fibers in actinic elastosis, skin specimens obtained from sun-damaged areas were immunohistochemically examined using the antibodies against acrolein and HNE. Both antibodies were found to react with the accumulations of elastic material. Double immunofluorescence labeling demonstrated that acrolein/elastin and HNE/elastin were colocalized in the actinic elastosis. Western blot analysis showed that the polypeptide with a molecular weight of 62 kDa reacted with anti-acrolein, anti-HNE and anti-elastin antibodies. The results suggest that acrolein and HNE may be associated with actinic elastosis.

Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds

“…These two approaches may also be combined. with proteins was also developed and tested around the same time [102,103]; subsequently other researchers produced an antibody against the same immunogen, and showed that it recognized acrolein-modified albumin [104]. Using this antiserum they developed an ELISA and reported limited cross-reactivity with adducts of formaldehyde, malondialdehyde, or 4-hydroxynonenal with albumin.…”

A simplified procedure for semi-targeted lipidomic analysis of oxidized phosphatidylcholines induced by UVA irradiation

“…For example, nonfragmented OxPLs induce barrier-protective effects in lung endothelium, whereas fragmented species are barrier disruptive ( 9 ). Similarly, exposure of dermal fi broblasts to UVA-generated, nonfragmented oxidized phosphatidylcholine (OxPCs) induced adaptive responses ( 10,11 ), whereas production of fragmented species containing reactive carbonyl groups correlated to skin ageing ( 12 ) and pathologies of the skin that develop upon solar exposure, such as actinic elastosis ( 13 ).…”

Molecular pathogenesis of emphysema

“…In addition, the damaged lung may "remember" the accumulated inflicted injury. For example, in damaged skin, colocalization of elastin and the oxidative stress marker 4-hydroxy-nonenal has been identified, as well as colocalization of elastin and the cell toxin acrolein (116). Although acrolein can upregulate endothelial cell heme oxygenase-1 (117), it is also one of the most chemically reactive aldehydes inhaled with cigarette smoke (118), and lysyl-acroleinated proteins have been detected in plasma samples from smokers (119).…”