Immunohistochemical Detection of JC Virus in Nontumorous Renal Tissue of a Patient with Renal Cancer but without Progressive Multifocal Leukoencephalopathy

Aoki, Naoto; Kitamura, Tadaichi; Tominaga, Takashi; Fukumori, Nobutaka; Sakamoto, Yosimitsu; Kato, Kenzo; Mori, Mayumi

doi:10.1128/jcm.37.4.1165-1167.1999

Cited by 9 publications

(3 citation statements)

References 23 publications

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“…In our cases, the immunohistochemically positive cells with morphological evidence of viral infection were randomly distributed in the renal parenchyma and focally involved renal tubules. These findings are similar to those reported by Aoki et al (32) and Dorries and ter Meulen (33). in surgical samples, but different from those of Randhawa et al (11), who reported the selective infection of re- nal cortical parenchyma by JC virus in a single kidney transplanted patient.…”

Section: Discussionsupporting

confidence: 87%

“…In all of these cases, nPCR of the LT region, followed by restriction fragment length polymorphism analysis, revealed the presence of JC virus, whereas BK virus sequences were never found. Like Aoki et al (32) and Dorries and ter Meulen (33), we did not find any inflammatory infiltration around the renal tubules containing JC virus-positive cells or casts. Randhawa et al (11) reported slight interstitial inflammatory infiltration in close proximity to inclusion-bearing cells stained with anti-JC virus VP1 antiserum, but their case was co-infected with BK virus, which may have been the cause of the inflammatory infiltration.…”

Section: Discussionsupporting

confidence: 84%

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Lytic JC Virus Infection in the Kidneys of AIDS Subjects

et al. 2003

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Our objective was to investigate the role of the human polyomavirus JC virus as a possible cause of renal damage in AIDS subjects. Histology, immunohistochemistry, and molecular biology were used to evaluate the frequency of viral infection, genotypes, viral status, and the presence of rearrangements or point mutations in specific genomic regions of strains isolated from renal tissue. Formalin-fixed, paraffin-embedded sections of postmortem renal specimens obtained from 111 unselected AIDS patients were stained for routine histology and with anti-SV40 antibody. The immunohistochemically positive specimens were further investigated by means of nested polymerase chain reaction for different polyomavirus genomic regions (large T, transcriptional control region, and viral protein 1). Furthermore, the sequences of transcriptional control region and viral protein 1 were also analyzed. Immunohistochemistry was positive in seven cases (6.3%), four of which showed morphological evidence of viral replication (intranuclear inclusion bodies and/or intratubular cellular casts): in all seven cases, only epithelial tubular cells (with and without inclusion bodies) and cellular casts were stained. The JC virus genome was identified by polymerase chain reaction in five of the seven immunohistochemically positive cases; transcriptional control region and viral protein 1 were amplified in, respectively, three and four cases. Transcriptional control region sequence analysis revealed major rearrangements in all three cases, with duplications of all the transcriptional factor-binding sites, whereas no point mutations were found in the viral protein 1 region, which was characterized as Type 1A in all cases. For the first time in AIDS subjects, this study shows that although rarely, JC virus can replicate in renal tissue. Molecular biology revealed major rearrangements in the transcriptional control region that, together with other unknown factors, could justify the increased pathogenicity of this human polyomavirus.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 84%

Lytic JC Virus Infection in the Kidneys of AIDS Subjects

et al. 2003

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“…Even though JCV infection has never revealed any histopathological changes in the kidney, viral DNA is commonly detected in the renal cortex and medulla of more than 50% of individuals. 20,54 We used the SNA and MAA lectins to probe 5.0-m-thick kidney sections for the presence of ␣2,6and ␣2,3-linked SA, respectively. Figure 6A shows that the receptor-type SA expression is high in the kidney demonstrated by staining of the renal glomerulus and the surrounding tubules.…”

Section: Expression Of the Jcv Receptor-type Sa In Human Kidney And Lungmentioning

confidence: 99%

Differential Distribution of the JC Virus Receptor-Type Sialic Acid in Normal Human Tissues

Eash

Tavares

Stopa

et al. 2004

The American Journal of Pathology

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JC virus (JCV), a member of the polyomavirus family, causes a demyelinating disease of the central nervous system (CNS) in humans known as progressive multifocal leukoencephalopathy. Although glial cells are the principal target of JCV productive infection in progressive multifocal leukoencephalopathy patients, little is known regarding the site of JCV persistence and the mechanisms by which the virus spreads to the CNS to cause disease. Previous work has demonstrated the presence of replicating JCV DNA in B lymphocytes from peripheral blood, tonsil, and spleen and it has been hypothesized that lymphocytes may be one site of JCV persistence. Detection of viral gene products in renal tubules and excretion of JC virions in the urine suggests JCV persistence in the kidney. A respiratory route of viral transmission has also been hypothesized implicating the lung as another possible site of persistent JCV infection. Earlier studies from our laboratory have shown that terminal alpha 2,6-linked sialic acid is a critical component of the JCV receptor. In this report we examined the tissue distribution of this JCV receptor-type sialic acid in a panel of normal human tissues. Our results demonstrate that in normal brain JCV receptor-type sialic acids are expressed on oligodendrocytes and astrocytes, but not on cortical neurons. The receptor-type sialic acid is also more highly expressed on B lymphocytes than on T lymphocytes in normal human spleen and tonsil. In addition, both the kidney and lung express abundant levels of alpha 2-6-linked sialic acids. Our data show a striking correlation between the expression of the JCV receptor-type sialic acid on cells and their susceptibility to infection by the virus. These findings also support the hypothesis of JCV persistence in lymphoid tissue and B-cell-facilitated viral dissemination to the CNS.

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Simian virus 40 regulatory region structural diversity and the association of viral archetypal regulatory regions with human brain tumors

Lednicky

Butel

2001

Seminars in Cancer Biology

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Immunohistochemical Detection of JC Virus in Nontumorous Renal Tissue of a Patient with Renal Cancer but without Progressive Multifocal Leukoencephalopathy

Cited by 9 publications

References 23 publications

Lytic JC Virus Infection in the Kidneys of AIDS Subjects

Lytic JC Virus Infection in the Kidneys of AIDS Subjects

Differential Distribution of the JC Virus Receptor-Type Sialic Acid in Normal Human Tissues

Simian virus 40 regulatory region structural diversity and the association of viral archetypal regulatory regions with human brain tumors

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