Immunohistochemical Detection of Interleukin-12 and Interferon-γ in Pigs Experimentally Infected with Mycoplasma hyopneumoniae

Mycoplasma pneumonia of swine (MPS) lung lesions and immunogenic properties were compared between a Landrace line that was genetically selected for reduced incidence of pulmonary MPS lesions, and a non-selected Landrace line. The MPS-selected Landrace line showed significantly lower degrees of pulmonary MPS lesions compared with the non-selected Landrace line. When changes in immunity before and after vaccination were compared, the percentage of B cells in the peripheral blood of the MPS-selected Landrace line was significantly lower than that of the non-selected line. Furthermore, the concentration of growth hormone and the mitogen activity of peripheral blood mononuclear cells in the MPS-selected Landrace line showed significantly (P < 0.05) lower increases after vaccination than the non-selected line. Conversely, the concentration of peripheral blood interferon (IFN)-γ and salivary immunoglobulin A (IgA) after Mycoplasma hyopneumoniae vaccination was significantly higher in the MPS-selected Landrace line than in the non-selected line. Gene expression of toll-like receptor (TLR)2 and TLR4 was significantly higher in the MPS-selected Landrace line in immune tissues, with the exception of the hilar lymph nodes. The present results suggest that peripheral blood IFN-γ, salivary IgA TLR2, and TLR4 are important immunological factors influencing the development of MPS lesions.

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“…; Rodriguez et al . ). Furthermore, cell‐mediated immunity is important for controlling MPS (Thacker et al .…”

Section: Discussionmentioning

confidence: 97%

Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine

Borjigin

Shimazu

Katayama

et al. 2015

Animal Science Journal

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“…Although numerous functions and roles of IL-17 have been reported in mice and humans (Bailey 2009), there have been few reports on the role of IL-17 in pigs. IFN-γ is mainly produced by Th1-type T cells and mediates cellular immunity under Mhp infection, promoting the proliferation of macrophage to repair damaged cells (Rodríguez et al 2007). However, there are no reports on the relationship between Mhp or MPS and IL-17.…”

Section: Genetic Trendsmentioning

confidence: 99%

“…Therefore, the strong negative genetic correlation between MPS lesions and IL-17 in the present study represents an important finding. IFN-γ is mainly produced by Th1-type T cells and mediates cellular immunity under Mhp infection, promoting the proliferation of macrophage to repair damaged cells (Rodríguez et al 2007). Shimazu et al (2014) previously reported that IFN-γ concentration in serum after MPS vaccination was significantly higher in the MPS-selected Landrace line compared with a general, non-selected Landrace line.…”

Section: Genetic Trendsmentioning

confidence: 99%

Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs

Sato

Okamura

Kojima-Shibata³

et al. 2015

Animal Science Journal

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Mycoplasma pneumonia of swine (MPS) is responsible for significant economic losses in the swine industry. We selected Landrace pigs for reduced MPS pulmonary lesions over five generations, and measured concentrations of the following cytokines: interleukin (IL)-10, IL-13, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-γ to estimate their correlation with MPS lesions. Sheep red blood cells (SRBC) were injected twice intramuscularly at 70 and 95 kg body weight. Blood serum samples were collected after 1 week of secondary SRBC inoculation and cytokine concentrations were analyzed by ELISA. Genetic parameters and breeding values were estimated. The heritability estimates of IL-10, IL-13, IL-17, TNF-α and IFN-γ were 0.20 ± 0.06, 0.12 ± 0.06, 0.27 ± 0.07, 0.20 ± 0.10 and 0.05 ± 0.03, respectively. Genetic correlations of IL-17 and TNF-α with pulmonary MPS lesions were high (-0.86 ± 0.13 and 0.69 ± 0.29, respectively) and those of IFN-γ and IL-13 with MPS lesions were moderately negative (-0.45). Through selection, the breeding values of IL-17 and IFN-γ increased substantially and those of TNF-α decreased. These results suggest that innate and cellular immunity are more important for the suppression of pulmonary lesions in MPS than humoral-mediated immunity, such as antibody response.

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“…IL‐12 and interferon (IFN)‐γ are known to control the cellular immune type 1 response, and they are also involved in Mhp infection (Rodriguez et al . ). Hormones are also involved in immune reactions (Steinman ; Webster Marketon & Glaser ), although their role is not as clear as that of cytokines.…”

Section: Introductionmentioning

confidence: 97%

Genetic selection for resistance to mycoplasmal pneumonia of swine (MPS) in the Landrace line influences the expression of soluble factors in blood after MPS vaccine sensitization

Shimazu

Borjigin

Katayama

et al. 2013

Animal Science Journal

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We recently developed a Landrace line that is resistant to mycoplasmal pneumonia of swine (MPS) infection by genetic selection for five generations, and we reported that the immunophenotype of this line is different from that of the non-selected line in terms of changes in peripheral blood leukocyte population after MPS vaccination. This study followed up previous findings demonstrating changes in soluble factors in blood, namely, hormones, Mycoplasma hyopneumoniae-specific immunoglobulin G (IgG), and cytokines. These two lines were injected with MPS vaccine on days -7 and 0 after blood sampling on those days, and blood samples were collected on days -14, -7, 0, 2, 7 and 14. We found changes in the levels of many hormones and cytokines in both lines. However, we found that only growth hormone (GH) and interferon (IFN)-γ levels were statistically different between these two lines. GH concentration was reduced (day 0) and IFN-γ concentration was increased (day 14) in the MPS-selected line compared with the non-selected line, despite unchanged IFN-γ messenger RNA expression in blood cells. Although detailed mechanisms underlying these phenotypes remain unsolved, these traits would be useful to improve MPS resistance in pig production and provide an insight into MPS infection.

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Immunohistochemical Detection of Interleukin-12 and Interferon-γ in Pigs Experimentally Infected with Mycoplasma hyopneumoniae

Cited by 20 publications

References 13 publications

Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine

Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine

Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs

Genetic selection for resistance to mycoplasmal pneumonia of swine (MPS) in the Landrace line influences the expression of soluble factors in blood after MPS vaccine sensitization

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