Immunohistochemical detection of Fas antigen in oral epithelia

Abstract

A monospecific and high titer polyclonal antibody, designated as Fas D, raised against synthetic polypeptides selected from a part of the human Fas antigen (aa 104-114), was used to identify the Fas antigen in human oral epithelia in normal and pathological states. The human gingival proteins had been extracted and analysed by an ABC (avidin-biotin complex) immunoblotting technique. The antibody interacted with a single band in gingival proteins with an estimated molecular weight of 35,000, which is in good agreement with that calculated from amino acid sequences of the human Fas antigen. Using an indirect immunohistochemical method, the antibody localized on the stratum spinosum and the basal part of the stratum corneum of normal human gingiva. Specimens obtained from patients with odontogenic keratocysts, leukoplakia, lichen planus, and squamous cell carcinoma were also stained with the antibody. The pattern of the Fas antigen distribution in oral stratified squamous epithelia was, with some overlapping, characteristic for each disease.

Differential expression of apoptosis-related proteins in various cellular components of ameloblastomas

“…In contrast to the present results, the authors found that Fas, FasL and caspase-3 reactivity in peripheral columnar or cuboidal cells was more intense than that in central polyhedral cells; this requires further study. MURAKI et al 16 demonstrated in their study that Fas-positive cells were found in leukoplakia with keratosis and in well-differentiated squamous cell carcinoma, suggesting that the Fas antigen may be related to the keratinization of some types of lesions. Taken together with the present results, the expression of both Fas and FasL in ameloblastomas appears to be associated with certain features of differentiation or degeneration of tumour cells.…”

“…Recent studies have detected apoptotic cells and several apoptosis-related proteins, such as Bcl-2 family gene products, Fas, Fas-ligand (FasL) and caspase-3, in developing tooth germs, odontogenic keratocysts and ameloblastomas 4,[6][7][8][9]14,16,19 , suggesting that apoptosis may play important roles in normal odontogenesis as well as in the pathogenesis of odontogenic lesions. The aim of this study was to evaluate the expression pattern of apoptosis-related proteins in different histological subtypes and various cellular components of ameloblastomas.…”

Fas ligand expression in esophageal carcinomas and their lymph node metastases

“…20 Previously, it has been shown that squamous cell carcinomas of the tongue and oral cavity, lung, and squamous cell carcinoma cell lines express Fas. [21][22][23][24] In a recent study, however, Fas expression in ESCCA was found to be heterogeneous and essentially absent in the majority of cases, although it was expressed in the Immunoperoxidase staining was done with hematoxylin counterstaining (original magnification ϫ100).…”

Regulation of CD95 (APO-1/ FAS) ligand and receptor expression in squamous-cell carcinoma by interferon-? and cisplatin

“…Derangement of the CD95 system is critical for tissue damage of UV-radiated skin and for dermic injury originating from cutaneous lupus erythematodes (Nakajima et al, 1997). In contrast to malignant melanoma and basal cell epithelioma, CD95 receptor expression was present in squamous cell carcinoma (SCC) as well as in normal skin (Muraki et al, 1997). High levels of CD95L expression were typically observed during tumor progression and immune evasion of malignant melanoma (Hahne et al, 1996).…”