Immunohistochemical detection of collagen type III and IV in relation with transformation of Ito cells in liver sinusoids of patients with reactive biliary hepatitis

Gulubova, Maya; Stoyanov, Hristo D.; Julianov, Alexander; Vasilev, I; Stoyanova, Irina; Hadjipetkov, Philip

doi:10.1016/s0065-1281(99)80020-8

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…The number of Ito cells (fat-storing cells) increases periportally and around the central veins in reactive biliary hepatitis [6] and this increase has been suggested to be related to hepatic fibrosis in humans and other mammals [7,10]. In the normal human liver, the cells can be identified by the presence of lipid droplets and expression of α-smooth muscle actin [7].…”

mentioning

confidence: 99%

Immunohistochemical and Ultrastructural Study of Ito Cells(Fat-Storing Cells) in Response to Extrahepatic Bile Duct Ligation in Broiler Chickens.

Handharyani

Ochiai

Iwata

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. The Ito cell (fat-storing cell) lies in perisinusoidal space of liver and has a variety of functions. We investigated the immunohistochemistry and ultrastructure of Ito cells in normal and cholestatic livers of broiler chickens. Immunohistochemistry demonstrated that Ito cells expressed HHF35 muscle actin, vimentin, desmin, glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), chromogranin A and cytokeratins in normal livers. These cells were diffusely scattered throughout the lobules. Livers treated with extrahepatic bile duct ligation (BDL) showed cholestasis, fibrosis, proliferation of biliary ductules and Ito cells. The Ito cells were frequently found in fibrotic areas and were larger in size with more extensive immunoreactivity than those of normal livers. Ultrastructural study demonstrated that Ito cells were closely associated with the production of collagen fibers in BDL livers. These findings suggest that Ito cells actively react against hepatocytic injuries and play a major role in the hepatic fibrogenesis of cholestatic livers of chickens. KEY WORDS: bile duct ligation, broiler, immunohistochemistry, Ito cell, ultrastructure.

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confidence: 99%

Immunohistochemical and Ultrastructural Study of Ito Cells(Fat-Storing Cells) in Response to Extrahepatic Bile Duct Ligation in Broiler Chickens.

Handharyani

Ochiai

Iwata

et al. 2001

The Journal of Veterinary Medical Science

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“…In people, chronic liver diseases, including livers with cholestasis, are accompanied by newly formed intrahepatic bile ductules and a -smooth muscle actin-positive HSCs (Hines et al, 1993). The HSCs can proliferate, transform into myofibroblasts and play a major role in fibrotic activity (Enzan et al, 1994;Gulubova et al, 1999). The present results suggest that HSCs in birds have cytological functions in hepatic fibrosis similar to those in mammals.…”

Section: Discussionmentioning

confidence: 58%

“…The cells expressing a -smooth muscle actin are unevenly distributed in hepatic lobules. In reactive cholangiohepatitis of people, which is a result of chronic diseases of the gall bladder, bile ducts or pancreas, bile acid retention in bile ducts may cause transformation of HSCs and increased collagen type III and type IV in the liver (Hines et al, 1993;Gulubova et al, 1999). However, the biological nature and the role of HSCs has not been sufficiently clarified in hepatic disorders of birds.…”

Section: Introductionmentioning

confidence: 99%

Idiopathic hepatic fibrosis with cholestasis in broiler chickens: Immunohistochemistry of hepatic stellate cells

2002

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Immunohistochemical examinations of hepatic stellate cells (HSCs) were performed on six enlarged livers from broiler chickens with malformation of the extrahepatic biliary tract (group 1) and on eight broiler livers affected with naturally occurring cholangiohepatitis without biliary malformation (group 2). The livers from both groups were grossly enlarged, firm and tan-coloured, and histologicall y revealed severe diffuse fibrosis with proliferation of bile ductules. HSCs positive for muscle actin and desmin actively proliferated in the perisinusoidal space and around newly formed bile ductules. There was no difference in the immunohistochemical reactivities and location of HSCs between the two groups. The findings suggest that the diffuse hepatic fibrosis found in group 2 as well as group 1 results from reactive proliferation of HSCs.

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“…Magnifications, a x 7 000, b x 12 000. changes such as moderate portal inflammation and fi brosis have been described in various conditions leading to nonspecific reactive hepatitis. [2] In some cases periductal fibrosis and slight cholestasis could be detected [17] . The presence of intense inflammatory infiltration in liver parenchyma surrounding the hydatid cysts could cause hepatocyte derangements and stimulation of fibrogenesis.…”

Section: Discussionmentioning

confidence: 99%

Liver tryptase-positive mast cells and fibrosis in children with hepatic echinococcosis

Gulubova

Vlaykova

Kalinova

et al. 2005

J Indian Assoc Pediatr Surg

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Immunohistochemical detection of collagen type III and IV in relation with transformation of Ito cells in liver sinusoids of patients with reactive biliary hepatitis

Cited by 5 publications

References 29 publications

Immunohistochemical and Ultrastructural Study of Ito Cells(Fat-Storing Cells) in Response to Extrahepatic Bile Duct Ligation in Broiler Chickens.

Immunohistochemical and Ultrastructural Study of Ito Cells(Fat-Storing Cells) in Response to Extrahepatic Bile Duct Ligation in Broiler Chickens.

Idiopathic hepatic fibrosis with cholestasis in broiler chickens: Immunohistochemistry of hepatic stellate cells

Liver tryptase-positive mast cells and fibrosis in children with hepatic echinococcosis

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