Immunohistochemical demonstration of collagenase and tissue inhibitor of metalloproteinases (TIMP) in synovial lining cells of rheumatoid synovium

Okada, Yasunori; Gonoji, Yukio; Nakanishi, Isao; Nagase, Hideaki; Hayakawa, Taro

doi:10.1007/bf02899418

Cited by 74 publications

(48 citation statements)

References 47 publications

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“…IL-6 promotes synovitis by inducing neovascularisation via vascular endothelial growth factor (VEGF)-stimulated pannus proliferation, resulting in infiltration of inflammatory cells and synovial hyperplasia [17]. In terms of joint erosion, IL-6 causes bone resorption by inducing osteoclast formation via the induction of RANKL in synovial cells [18,19], and cartilage degeneration by producing matrix metalloproteinases (MMPs) in synovial cells and chondrocytes [20,21].…”

Section: The Role Of Interleukin-6 In the Pathogenesis Of Rheumatoid mentioning

confidence: 99%

Targeting Interleukin-6 in Rheumatoid Arthritis

Yusof

Emery

2013

Drugs

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Section: The Role Of Interleukin-6 In the Pathogenesis Of Rheumatoid mentioning

confidence: 99%

Targeting Interleukin-6 in Rheumatoid Arthritis

Yusof

Emery

2013

Drugs

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“…Both MMP-1 and MMP-3 are known to be major cartilage-degrading enzymes which are produced by synovial lining cells and chondrocytes themselves [7,25,26]. Although recent studies have demonstrated the importance of other proteinases, such as aggrecanases, in cartilage destruction [16,28,32], MMP-1 and MMP-3 possibly play significant roles, since both enzymes have a wide variety of substrate specificity and are capable of degrading collagens and proteoglycans, which are major components of the cartilage matrix [21].…”

Section: Duration Of Pain (Months)mentioning

confidence: 99%

Biochemical markers in the synovial fluid of glenohumeral joints from patients with rotator cuff tear

Yoshihara

Hamada

Nakajima

et al. 2001

Journal Orthopaedic Research

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It is known that rotator cuff tears are sometimes accompanied by joint destruction. Our purpose was to elucidate the pathology with this condition. Thirty-two synovial fluid (SF) samples aspirated from the glenohumeral joints of patients with rotator cuff tears, including 7 with partial-thickness and 25 with full-thickness tears of the rotator cuff (10 massive and 15 isolated supraspinatus tendon (SSp) tears), were examined. Collagenase (MMP-l), stromelysin 1 (MMP-3), tissue inhibitor of metalloproteinases-1 (TIMP-1) and carboxy-terminal type I1 procollagen peptide (pCOL 11-C) were measured in the SF using the respective sandwich enzyme immunoassays. Glycosaminoglycan (GAG) was also quantified with a cationic dye binding method using 1.9-dimethylmethylene blue. Levels of any molecules except pCOL 11-C in the SF appeared to be higher in full-thickness tears than those in partial-thickness tears. Moreover, levels of MMP-1, MMP-3 and GAG in the SF were significantly higher in massive tears of the rotator cuff in comparison with those in isolated SSp tears. Such significance was not observed in the levels of TIMP-I or pCOL I1 C in the SF. We examined the relation of those levels with operative findings or clinical parameters from full-thickness tears, and observed significant correlations of the tear size with the levels of MMP-1, MMP-3 and GAG in the SF. Although these marker molecules in SF do not always originate from cartilage, our results may indicate the potential for accelerated cartilage-degrading activity in the glenohumeral joint in massive tears of the rotator cuff.

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“…The deparaffinized sections were preincubated with normal bovine serum to prevent nonspecific binding, and then incubated overnight at 4°C with an optimal dilution (0.5 g/mL) of a primary monoclonal antibody against human MMP-1 (F-67, Fuji Chemical Industries, Ltd., Toyama, Japan) (22). Thereafter, the slides were incubated with an alkaline phosphatase-conjugated goat anti-mouse immunoglobulin antibody.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…The reaction products were resolved using a mixture of 5-bromo-4-chloro-3-indolyl phosphate and nitroblue tetrazolium chloride (BCIP/NBT; DAKO, Carpinteria, CA). Rheumatoid arthritis tissue served as the positive control (22). Analysis of immunohistochemical staining was performed by two investigators (JS, MI).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Expression of Matrix Metalloproteinase-1 in Human Colorectal Carcinoma

et al. 2000

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Matrix metalloproteinases are considered to play an important role in tumor invasion and metastasis. To elucidate the involvement of MMP-1 in human colorectal carcinoma, we performed immunohistochemical analysis on tissues from 20 colorectal adenomas and 142 colorectal adenocarcinomas, including 27 intramucosal carcinomas and 115 invasive carcinomas. MMP-1 was not expressed in any of the 20 cases of colorectal adenoma examined. In contrast, 108 of 142 cases (76.1%) with colorectal adenocarcinoma showed immunoreactivity for MMP-1 in the carcinoma cells themselves. Expression of MMP-1 was also identified in stromal cells around the carcinoma. We investigated the relationship between pathological features in colorectal carcinoma and MMP-1 immunoreactivity of the tumor cells. MMP-1 expression was less frequent in intramucosal carcinomas and weaker than that in invasive carcinomas (P < .0001). Among the 115 cases of invasive carcinomas, MMP-1 immunoreactivity was significantly correlated with the depth grading of tumor invasion (P < .05), tumor growth pattern (P < .05), the presence of lymphatic invasion (P < .05), venous invasion (P < .05), neural invasion (P < .05), lymph node metastasis (P < .005), hepatic metastasis (P < .05), and increasing stages of Dukes' classification (P < .05). In situ hybridization, using an MMP-1 oligonucleotide probe, Colorectal carcinoma is the most common malignant tumor of the alimentary tract in the Western world. At the time of diagnosis, colorectal carcinoma usually shows extensive local invasion and metastasis. Enzymatic degradation of the extracellular matrix, such as basement membrane and interstitial stroma, is an essential step in tumor invasion and metastasis (1). Matrix metalloproteinases (MMPs) constitute a family of zinc-dependent enzymes that are involved in the degradation of the extracellular matrix (2). According to their structures and substrate specificities, MMPs can be classified into subgroups of collagenases (MMP-1, MMP-8, and MMP-13), gelatinases (MMP-2 and MMP-9), stromelysins (MMP-3, MMP-10, MMP-12, and MMP-7), membrane-type MMPs (MT-MMPs) and other MMPs. Currently, this family of MMPs has at least 17 different members (3). Tumor invasion is facilitated by degradation of the interstitial stroma, which is the main component of the extracellular matrix. This process can be furthered by collagenases, particularly interstitial collagenase (MMP-1) (4). A requirement for MMP-1 for tumor invasion has been demonstrated in in vitro assays (5). However, the tissue distribution and the role of MMP-1 in vivo are still controversial. Previously, MMP-1 was shown to be expressed mainly in stromal cells surrounding the carcinoma cells (6 -9). In contrast, recent studies have reported MMP-1 expression by the carcinoma cells themselves and suggested a relationship between tumor progression and this MMP-1 expression (10 -13). Murray et al. (11) have described that immunohistochemically detected MMP-1 expression by the tumor cells was associated with poor prognosis of patients w...

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Immunohistochemical demonstration of collagenase and tissue inhibitor of metalloproteinases (TIMP) in synovial lining cells of rheumatoid synovium

Cited by 74 publications

References 47 publications

Targeting Interleukin-6 in Rheumatoid Arthritis

Targeting Interleukin-6 in Rheumatoid Arthritis

Biochemical markers in the synovial fluid of glenohumeral joints from patients with rotator cuff tear

Expression of Matrix Metalloproteinase-1 in Human Colorectal Carcinoma

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