Immunohistochemical demonstration of apoptosis-regulated proteins, Bcl-2 and Bax, in resected non-small-cell lung cancers

Abstract

Bcl-2 overexpression was significantly associated with SCC. Spontaneous apoptosis was significantly associated with SCC and with negative nodal metastasis. Apoptosis-regulated proteins, Bcl-2 and Bax, and AI lack significant associations with each other and prognostic values in patients with resected NSCLC.

Molecular Profiling of Computed Tomography Screen-Detected Lung Nodules Shows Multiple Malignant Features

“…In agreement with the aforementioned cDNA study, the almost identical percentages of molecular alterations reported herein support the hypothesis that lung cancer detected in a screening program has a similar malignant potential than conventionally, nonscreen-detected cancer. Furthermore, all the data from both series are concordant with the published literature on resectable non -small cell lung cancer in relation to cell cycle -related proteins (18,21,23,24,33,(40)(41)(42), apoptosis regulators (26,43,44), invasive and angiogenic markers (26,27), RNA processing molecules (28,29), and genetic aberrations (15,38).…”

“…Thus, high levels of Ki-67 (18,19,40), loss of p16 (21), accumulation of p53 (45,46), cyclin E overexpression (40), and Bcl-2 expression (26,43,44,47) were observed more commonly in squamous cell carcinomas than in adenocarcinomas. However, p21 loss was observed more frequently in adenocarcinomas than in squamous cell carcinomas.…”

Prognostic value of the apoptotic index analysed jointly with selected cell cycle regulators and proliferation markers in non-small cell lung cancer

“…AI was defined as an amount of TUNEL positive cells per 1000 tumor cells, concordant with previously published methods [18,21,[23][24][25][26] and criteria [11,18,21,[23][24][25][26]. In the enlarged study group the mean and median AI remained similar to our previous study (12 vs. 14 and 8 vs. 9, respectively) [11], and within the range demonstrated by other authors (the mean AI range was 11-23, the median AI range was [8][9][10][11][12][13][14][15] [18,20,21,23,[26][27][28]. Similar to other authors [18,20,21], a larger number of cases allowed us to divide our group of patients into four subgroups, based on 25th, 50th and 75th percentile of AI.…”

Clinical significance of apoptotic index in non-small cell lung cancer: correlation with p53, mdm2, pRb and p21WAF1/CIP1 protein expression

“…We did not find any correlation between AI and patient characteristics. In other NSCLC series higher AI was associated with squamous (Hanaoka et al 2002) or adenous lung cancer subtype (To¨rma¨nen et al 1995). Tanaka et al (2002) reported that the highest AI was more common in moderate-or poor-differentiated than in well-differentiated NSCLC cases, whereas in lymphomas, breast, endometrial or thyroid carcinomas, higher apoptosis was associated with the less differentiated tumors (revied by Soini et al 1998).…”