Immunohistochemical criteria for endometrial receptivity in I/II stage endometriosis IVF-treated patients

Krylova, Yu.S.; Polyakova, V. O.; Kvetnoy, Igor M.; Kogan, Igor Yu.; Dzhemlikhanova, Lyailya Kh.; Niauri, Dariko A.; Gzgzyan, Alexandr; Ailamazyan, Eduard К.

doi:10.1080/09513590.2016.1232576

Cited by 12 publications

(5 citation statements)

References 9 publications

(8 reference statements)

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“…Western-blot and Synergy Brands, Inc (SYBR) green real-time- polymerase chain reaction (PCR) (Invitrogen) were used to test the expression of the marker for endometrial lining, cytokeratin (CK) 20 and endometrial receptivity marker, LIF. 21…”

Section: Methodsmentioning

confidence: 99%

Bone Marrow Mesenchymal Stem Cells (BMSCs) Restore Functional Endometrium in the Rat Model for Severe Asherman Syndrome

et al. 2019

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Objective: To investigate the feasibility to restore functional endometrium using bone marrow mesenchymal stem cells (BMSCs) in the Sprague Dawley (SD["SD" has been defined as both "Sprague Dawley" and "standard deviation." Please clarify which one is to be followed.]) rat model for Asherman syndrome (AS). Design: Basic research on treatment of AS utilizing an optimized rat model. Setting: University research laboratories. Animal(s): Sprague Dawley rat model in which AS was induced in accordance to an optimized protocol. Intervention(s): Bone marrow mesenchymal stem cells were harvested from the rat's bone marrow and labeled with green fluorescent protein (GFP) in the second passage of BMSCs. The fifth passage of GFP-labeled BMSCs was injected systemically through the tail vein in the optimized AS rat model. Main Outcome Measure(s): We examined the reproliferation of the endometrial lining and the expression of markers for endometrium and endometrial receptivity. The localization of engrafted GFPlabeled BMSCs was determined by a laser scanning confocal microscope and a fluorescence microscope. The number of pregnant rats and implanted embryos in each uterus was recorded to evaluate the function of endometrium. Result(s): We had demonstrated that in the in vivo experiments on our rat model for AS, the group which received BMSC injection had significantly improved reproductive outcomes-70% of these rats conceived, whereas none of the rats in the control group got pregnant (P < .01, w 2 test). The mean number of embryos undergoing implantation was 14 + 1.24 in the sham group and 7 + 5.70 in the BMSC group (Levene test, P ¼ .001). There was no significant difference between the groups from the time of coitus to conception. To further determine how BMSC injection could have resulted in the improved reproductive outcomes in rats with AS, we employed immunohistochemical techniques to examine the endometrium of these treated rats. On hematoxylin-eosin staining, we noted the reproliferation of all layers of the endometrium and with Masson staining, we noted significant reduction in fibrosis in the damaged endometrium of rats treated with BMSCs. Counterstaining for GFP and cytokeratin-positive cells was noted in the endometrial lining of treated rats, which might suggest the action of BMSCs in regenerating the damaged endometrial lining. The expression of the endometrial receptivity marker, Leukemia inhibitory factor (LIF), in this regenerated endometrial lining could have resulted in the improved reproductive outcomes observed in the AS rat model treated with BMSCs. Conclusion: Bone marrow mesenchymal stem cells were likely to play an important role in the reconstruction of the injured endometrium and improvement of reproductive outcomes in the optimized AS rat model.

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Section: Methodsmentioning

confidence: 99%

Bone Marrow Mesenchymal Stem Cells (BMSCs) Restore Functional Endometrium in the Rat Model for Severe Asherman Syndrome

et al. 2019

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“…The 'window of implantation' (WOI) is defined as a period when the uterus is receptive for implantation of embryo. WOI is characterized by histological and morphological changes of endometrium and ER is determined by several factors [8]. It should be recognized that at the moment there is no single and optimal marker of ER, because of lack of accurate and objective methods of ER assessment [9].…”

Section: Introductionmentioning

confidence: 99%

The role of abnormal hypermethylation of the HOXA10 and HOXA11 promoters in implantation failures in IVF programs

Nazarenko

Kalinina

Knyazeva

et al. 2019

Gynecological Endocrinology

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Objective: The objective of this study is to investigate the association between methylation levels of HOXA10 and HOXA11 promoters, clinical parameters, and implantation outcomes after in vitro fertilizationembryo transfer (IVT-ET) cycles in women with repeated implantation failures and tubal infertility. Methods: Endometrium samples were collected from 34 women during implantation window before IVF-ET cycle to assess methylation status of HOXA10 and HOXA11 promoters using bisulfite sequencing. All participants had a tubal factor of infertility and at least two implantation failures in the anamnesis. A logistic regression model was used to predict the implantation outcome depending on methylation status and clinical parameters. Results: The methylation of CpG-islands of HOXA10 and HOXA11 promoters was identified in 76.5 and 100% of participants, respectively. The median methylation levels did not differ significantly between the groups with different implantation outcomes, but a logistic regression model based on HOXA10 and HOXA11 methylation and clinical parameters allowed to classify the implantation outcomes with the total percentage of correct predictions of 85.19%. Conclusions: Abnormal methylation levels of the HOXA10 and HOXA11 promoters were found in the endometrium of women with tubal infertility and repeated implantation failures. The findings suggest that methylation status could be an important factor of implantation failure during IVF-ET cycles.

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“…[ 12 ] Research has increasingly shown that endometrial receptivity in patients with EMs is significantly decreased and accompanied by implantation failure and infertility. [ 13 ]…”

Section: Discussionmentioning

confidence: 99%

“…[12] Research has increasingly shown that endometrial receptivity in patients with EMs is significantly decreased and accompanied by implantation failure and infertility. [13] Our previous work concluded that the rates of embryo implantation and clinical pregnancy were significantly lower in patients with EMs who received IVF/intracytoplasmic sperm injection treatment than in other patients. [7] In this study, we mainly recruited patients under 38 years of age to eliminate age-related factors, based on the fact that older patients maintain a low pregnancy rate even after IVF/intracytoplasmic sperm injection treatment.…”

Section: The Mechanisms Of Ems-induced Infertilitymentioning

confidence: 99%

Factors affecting clinical outcomes after IVF-ET for infertile young patients with ovarian endometrioma: A 5-year retrospective cohort study

et al. 2022

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This study aimed to compare ovarian reserve function and outcomes after in vitro fertilization and embryo transfer (IVF-ET) for young women with pelvic endometriosis with or without ovarian endometrioma. We explored the main factors influencing pregnancy outcomes in young patients with endometrioma. A total of 619 patients ≤38 years of age who underwent IVF-ET in our reproductive center between January 2011 and December 2015 were recruited. Among these patients, 398 had pelvic endometriosis with ovarian endometrioma and 221 had pelvic endometriosis without ovarian endometrioma. Patients underwent ovulation induction during IVF-ET. The general conditions and clinical outcomes of IVF-ET treatment were compared. Key factors affecting the success of IVF-ET treatment for endometriomas were analyzed. During IVF-ET treatment, the numbers of retrieved oocytes and 2-pronuclei (2PN) embryos in all age groups ( P < .01), and the number of 2PN high-quality embryos in patients under 30 years of age was lower in the pelvic endometriosis with ovarian endometrioma group than in the pelvic endometriosis alone group ( P < .05). Logistic regression analysis showed the number of antral follicles, basal follicle-stimulating hormone (bFSH) levels, number of oocytes, number of 2PN embryos, and number of 2PN high-quality embryos were significantly related to the successful outcome of IVF-ET. Among these, the number of 2PN high-quality embryos was the only independent predictive factor. Ovarian endometrioma significantly impairs ovarian reserve function and ultimately affects the therapeutic efficacy of IVF-ET. Obtaining more 2PN high-quality embryos was important for IVF-ET treatment of young patients with ovarian endometriomas.

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Immunohistochemical criteria for endometrial receptivity in I/II stage endometriosis IVF-treated patients

Cited by 12 publications

References 9 publications

Bone Marrow Mesenchymal Stem Cells (BMSCs) Restore Functional Endometrium in the Rat Model for Severe Asherman Syndrome

Bone Marrow Mesenchymal Stem Cells (BMSCs) Restore Functional Endometrium in the Rat Model for Severe Asherman Syndrome

The role of abnormal hypermethylation of the HOXA10 and HOXA11 promoters in implantation failures in IVF programs

Factors affecting clinical outcomes after IVF-ET for infertile young patients with ovarian endometrioma: A 5-year retrospective cohort study

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