Immunohistochemical Characterization of the Cellular Infiltration in Asthmatic Bronchi

Poston, Robin N.; Chanez, Pascal; Lacoste, J.-Y.; Litchfield, T.M.; Lee, T. H.; Bousquet, Jean

doi:10.1164/ajrccm/145.4_pt_1.918

Cited by 222 publications

(119 citation statements)

References 13 publications

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“…The lymphocytes are essentially all T-cells, and show increased activation compared to controls [11][12][13]15]. In the present study, we have demonstrated that lymphocyte numbers are increased both in cartilaginous and membranous airways although cell counts increase with airway size.…”

Section: Discussionsupporting

confidence: 53%

“…Increased numbers of polymorphonuclear cells (mainly eosinophils) and mononuclear cells (mainly lymphocytes) have been identified at autopsy in the airways of patients dying of asthma [1][2][3] and in patients with less severe asthma, by use of bronchoalveolar lavage (BAL) [4][5][6][7] and biopsy of proximal airways [7][8][9][10][11][12][13][14][15]. Immunohistochemical techniques have identified the CD4+ subset of T-lymphocytes in the airways of patients with asthma, and have demonstrated that these lymphocytes and eosinophils express markers of functional activation to a greater extent than those from nonasthmatic subjects [11,12,15,16].…”

mentioning

confidence: 99%

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The distribution of eosinophils and lymphocytes in the large and small airways of asthmatics

Carroll

Cooke²,

James³

1997

Eur Respir J

246

163

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Airway inflammation in asthma consists of variably increased numbers of mononuclear and polymorphonuclear cells, and there is a growing body of evidence to suggest a primary role for lymphocytes and eosinophils in the pathogenesis of asthma. The aim of this study was to examine the distribution of lymphocytes and eosinophils in the bronchial tree of cases of mild and severe asthma.In cases of fatal asthma (n=10), nonfatal asthma (sudden nonrespiratory death with a history of asthma n=10), and control cases (sudden death with no history of respiratory illness, n=10), lymphocytes and eosinophils were counted in transverse sections of large and small airways stained with haematoxylin and eosin.Cases of fatal asthma had longstanding severe asthma and nonfatal cases generally had mild asthma. Lymphocytes were increased in all airway size groups both in fatal and nonfatal cases of asthma compared to control cases (p<0.001). Eosinophils were increased (p<0.001) in all airway size groups in the cases of fatal asthma compared to the nonfatal asthma and control groups, which were similar. These differences between case groups were of similar magnitude, when only a random single airway section from each case in each size group was examined. The numbers of lymphocytes correlated with the number of eosinophils in the fatal asthma group (r=0.60; p<0.0001), and to a lesser extent in the nonfatal (r=0.34; p=0.001) and control groups (r=0.32; p=0.001).These findings suggest that increased numbers of lymphocytes are uniformly distributed in the large and small airways in cases of asthma, independently of asthma severity, and that eosinophil recruitment may be related to asthma severity. Hence, biopsy specimens of the proximal airways are likely to be representative of the cellular infiltrate in large and small airways in mild and severe asthma.

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Section: Discussionsupporting

confidence: 53%

mentioning

confidence: 99%

The distribution of eosinophils and lymphocytes in the large and small airways of asthmatics

Carroll

Cooke²,

James³

1997

Eur Respir J

246

163

View full text Add to dashboard Cite

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“…Endobronchial biopsy has been widely used as a research tool to assess airway inflammation in asthma [3,4,[6][7][8][9][10][11][12][13][14][15][16]22]. Its use as a tool in clinical research has recently become the subject of review [20].…”

Section: Discussionmentioning

confidence: 99%

“…Biopsy studies of airway inflammation have generally used only two or three biopsies per case to enumerate a particular cell type [3,4,[6][7][8][9][10][12][13][14][15][16], and have often relied on 2D cell profile methods, expressing density as cells per area [4,[8][9][10][11][12][14][15][16].…”

Section: Inflammation In Bronchial Biopsies ML Carroll Et Almentioning

confidence: 99%

“…Since most biopsy studies reporting the density of inflammatory cells have been in mild cases of asthma [3,4,[6][7][8][9][10][11][12][13][14][15][16]22], comparisons with post mortem studies are effectively limited to the small number of such studies that have compared transverse sections from cases of less severe, nonfatal asthma and controls [23,24]. Results of biopsy studies in mild asthma have not been entirely consistent with either results of the post mortem studies using cases of nonfatal asthma, or with each other.…”

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confidence: 99%

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Do bronchial biopsies represent mast cell density in airways? A stereological study

Carroll

Carroll²

2006

European Respiratory Journal

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Endobronchial biopsy specimens may not adequately represent inflammatory cell counts throughout the airway wall. The present study aimed to compare mast cell density in biopsies and airway sections using both stereological and nonstereological methods.Post mortem biopsies and adjacent transverse sections were obtained from a mean of five proximal airways per case in 10 subjects who had died of nonrespiratory causes. Tryptasepositive mast cells were measured stereologically in 30-mm sections and nonstereologically in 5-mm sections using an optical disector (cells?mm ), respectively. Reference areas included the inner and total airway wall and to 100 mm below the basement membrane.Case means, based on four or more biopsy sites, significantly correlated with those on transverse sections for counts over the inner airway wall only, using both stereological and nonstereological methods. Cells?mm -3 and cells?mm -2 were significantly correlated within all reference areas. When endobronchial biopsies are obtained from at least four proximal airways per case, intersubject comparisons of mean mast cell density in the inner airway wall are as well represented by counts on biopsies as they are on transverse sections. This is the case using either threedimensional, stereological or two-dimensional, nonstereological methods.

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Asthma

Tolomeo¹,

Baker²,

Weiss³

2011

Nursing Care in Pediatric Respiratory Disease

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Immunohistochemical Characterization of the Cellular Infiltration in Asthmatic Bronchi

Cited by 222 publications

References 13 publications

The distribution of eosinophils and lymphocytes in the large and small airways of asthmatics

The distribution of eosinophils and lymphocytes in the large and small airways of asthmatics

Do bronchial biopsies represent mast cell density in airways? A stereological study

Asthma

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