Immunohistochemical characterization of superior cervical ganglion neurons supplying porcine parotid salivary gland

Wojtkiewicz, Joanna; Juranek, Judyta K.; Kowalski, Ireneusz M.; Bladowski, Marek; Całka, Jarosław; Majewski, Mariusz

doi:10.1016/j.neulet.2011.05.242

Cited by 15 publications

(20 citation statements)

References 46 publications

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“…In this study, single VIP-IR neurons were TH-negative and ChAT-negative, whereas TH-immunoreactivity in VIP-immunoreactive neurons of the human www.fhc.viamedica.pl lumbar ganglia [26] and porcine SCG [23,25] were not identified. The coexistence of DBH-and VIP-IR neurons was not identified in the paravertebral and prevertebral sympathetic ganglia of the cat [27], while the colocalization of DBH-and VIP-immunoreactivity was indicated only in neurons of thoracic ganglia in the pig [24].…”

Section: Discussioncontrasting

confidence: 61%

“…In the present study, all neurons which showed TH-immunoreactivity were also NPY-IR, while in the neurons of the porcine superior cervical ganglion (SCG), only small population of nerve cells contained both TH and NPY [23]. In our study, only single NPY-IR neurons were immunonegative for DBH.…”

Section: Discussionsupporting

confidence: 46%

“…No GAL-immunoreactive nerve cells were found in the thoracolumbar [11] and stellate ganglia of the pig [10], while in the porcine CSMG only solitary GAL-IR neurons were detected [17]. In our study, double-immunofluorescence revealed that only single GAL-positive neurons did not show TH-IR, indicating that these cells are non-noradrenergic, whereas virtually all of the GAL-IR cell bodies in the inferior mesenteric ganglion of pig [29] did not show immunoreactivity to TH or only small population of the porcine SCG contained TH and GAL [23].…”

Section: Discussionmentioning

confidence: 48%

“…In the porcine thoracic ganglia several clusters of 4 or more VIP-IR cells were described [24], and a small number of VIP-IR ganglionic cells in the cat superior mesenteric ganglion [12] as well as the SG of newborn and young mice and rats were observed [21]. In contrast, numerous cell were present in the canine SG and MCG [22], while VIP-IR neurons were not demonstrated in the ovine MCG [14] and porcine SCG [23,25]. In the present study, single neurons displayed immunoreactivity to VIP.…”

Section: Discussionmentioning

confidence: 99%

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Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Nourinezhad

Podlasz

Wąsowicz

2018

Folia Histochem Cytobiol.

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Introduction. Greater splanchnic nerve (GSN) is by far the largest of the splanchnic nerves and connects the paravertebral and prevertebral ganglia to transmit the majority of nociceptive information from the viscera. Despite its importance, the immunohistochemical features of the porcine GSN neurons have not yet been examined. Therefore, the aim of the study was to investigate the neurochemistry of the porcine GSN neurons and to compare their neurochemical coding with those of the paravertebral and prevertebral ganglia. Material and methods. Four gilts of Large White Polish breed were examined in this study. Antibodies to tyrosine hydroxylase (TH), dopamine b-hydroxylase (DBH), choline acetyltransferase (ChAT), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), galanin (GAL), methionine-enkephalin (MET), calcitonin gene-related peptide (CGRP), and substance P (SP) were used for immunohistochemical detection of classical neurotransmitters marker enzymes and neuropeptides in neuronal cell bodies of the GSN. Results. Double-labeling immunofluorescence revealed that virtually all GSN neurons exhibited the presence of catecholamine-synthesizing enzymes (TH/DBH-positive) and subpopulations of neurons contained immunoreactivity to NPY, VIP, SOM, GAL and MET. However, CGRP and SP-immunoreactivity were not observed in neuronal somata. Conclusions. Our data strongly suggest that the general immunohistochemical characterization of ganglion cells in the porcine greater splanchnic nerve is similar to that of the prevertebral ganglia (e.g. celiacomesenteric ganglion).

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Section: Discussioncontrasting

confidence: 61%

Section: Discussionsupporting

confidence: 46%

Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

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Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Nourinezhad

Podlasz

Wąsowicz

2018

Folia Histochem Cytobiol.

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“…There are available data showing the existence of different targets for NPY immunoreactive fibers of sympathetic and parasympathetic origin. Experiments using retrograde neuronal tracing technique combined with immunohistochemistry to identify NPY immunoreactive fibers in the rat submandibular and parotid glands 29,30 and porcine parotid glands 31 showed that the NPY sympathetic innervation is confined to the blood vessels of the glands, while periacinar NPY fibers have a parasympathetic origin. In the TMJ, it may also be possible that sympathetic NPY innervation is related to blood vessels while parasympathetic NPY nerve fibers innervate mainly the synovial membrane.…”

Section: Npy Immunoreactive Neuronal Profiles That Send Nerve Fibers mentioning

confidence: 99%

Distribuição dos neurônios e campos terminais que expressam a urocortina 3 no sistema nervoso central de primata não-humano (Cebus apella).

Batagello¹

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Distribution of alpha‐synuclein in rat salivary glands

Nishitani,

Mitoh,

Yajima

et al. 2024

The Anatomical Record

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Expression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition.

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Immunohistochemical characterization of superior cervical ganglion neurons supplying porcine parotid salivary gland

Cited by 15 publications

References 46 publications

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Distribuição dos neurônios e campos terminais que expressam a urocortina 3 no sistema nervoso central de primata não-humano (Cebus apella).

Distribution of alpha‐synuclein in rat salivary glands

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