Immunohistochemical characterization of intraocular metastasis of a canine transmissible venereal tumor

Pereira, Joabe dos Santos; Silva, A.B.F.; Martins, Alanda Lopes Baptista; Ferreira, Ana Maria Reis; Brooks, Dennis

doi:10.1046/j.1463-5224.2000.00097.x

Cited by 36 publications

(31 citation statements)

References 9 publications

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“…Vimentin is a marker of mesenchymal cells and is expressed in the TVT (Pereira et al 2000), in the MCT (Jakab et al 2009, Lin et al 2010, in the PCT (Baer et al 1989, Schrenzel et al 1998) and in the LHS (Azakami et al 2006). In this study, the tumours deϐined as TVT by IHC were positive for vimentin in 100% (6/6) of the cases and positive for CD45 in 28.6% (2/7) of the cases.…”

Section: Discussionsupporting

confidence: 47%

“…It is suggested that natural cutaneous implantation occurs in bites or other wounds (Schlafer & Miller 2007). Speciϐic TVT cell markers have not been described, but immunostaining for vimentin (Pereira et al 2000), lysozyme (Park et al, 2006), CD45 (Gross et al 2006) and CD45RA (Gross et al 2006) associated with histomorphological analysis support the diagnosis of this neoplasm.…”

Section: Introductionmentioning

confidence: 99%

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Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Araújo

Preis

Lavalle³

et al. 2012

Pesq. Vet. Bras.

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Pesq. Vet. , cytokeratin, vimentin and E-cadherin) on formalin-ϐixed, parafϐin-embedded sections of canine cutaneous round cell tumours. Neoplastic tumours were diagnosed by histology and histochemical stains and included 107 mast cell tumours, 31 cutaneous histiocytomas, two localized histiocytic sarcomas, 21 cutaneous lymphomas, three plasma cell tumours, one transmissible venereal tumour and seven unclassiϐied round cell tumours. The histologic diagnosis was modiϐied in 39.5% of the total 172 neoplasms. The staining for CD45 and Ecadherin were variable, and therefore, the ϐinal diagnoses of cutaneous histiocytoma and localized histiocytic sarcoma were made based on histology in association with negative results for CD3, CD79a, CD117 and cytokeratin. The cellular origin of unclassiϐied round cell tumours was deϐined in all cases. Cutaneous B-cell lymphoma and plasma cell tumours were CD79a-positive and could be distinguished from each other by the morphological characteristics. Mast cell tumours and T cell lymphoma were CD117 and CD3 positive, respectively. The positive staining for vimentin and the negative staining for CD3, CD79a, CD117 and cytokeratin favoured the diagnosis of transmissible venereal tumours. Thus, the ϐinal diagnosis of cutaneous round cell tumours should be based on the interpretation of immunohistochemical results together with the cellular morphology observed by histology. Therefore, more studies to optimize the speciϐic markers in formalin-ϐixed, parafϐin-embedded tissues (especially for histiocytes) are required for deϐinitive diagnosis of round cell tumours in dogs. las redondas em cães. Os tumores foram diagnosticados usando-se a histopatologia e a marcação imuno-histoquí-mica. Foram incluídos 107 mastocitomas, 31 histiocitomas cutâneos, 2 sarcomas histiocíticos localizados, 21 linfomas cutâneos, 3 plasmocitomas, 1 tumor venéreo transmissí-vel e 7 tumores de células redondas não classiϐicados. O diagnóstico histológico foi modiϐicado em 39,5% do total de 172 neoplasias. A marcação do anticorpo CD45 e E--caderina foi variável e, nesse sentido, o diagnóstico ϐinal de histiocitoma cutâneo e sarcoma histiocítico localizado foi baseado na histologia em associação com os resultados negativos para CD3, CD79a, CD117 e citoqueratina. A origem celular dos tumores de células redondas não classiϐicados foi deϐinida em todos os casos. foram distinguidos entre si pelas características morfológi-cas. Marcação positiva para vimentina e negativa para CD3, CD79a, CD117 e citoqueratina favoreceram o diagnóstico dos tumores venereos transmissíveis. Assim, o diagnósti-co ϐinal dos tumores de células redondas foram baseados na interpretação dos resultados da imuno-histoquímica em conjunto com a avaliação das características morfoló-gicas observadas na histologia. Finalmente, mais estudos em relação à padronização de marcadores especíϐicos para tecidos paraϐinizados (especialmente para histiócitos) são necessários para o diagnóstico deϐinitivo das neoplasias de células redondas em cães.TERMOS DE INDEXAÇÃO...

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Section: Discussionsupporting

confidence: 47%

Section: Introductionmentioning

confidence: 99%

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Araújo

Preis

Lavalle³

et al. 2012

Pesq. Vet. Bras.

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“…At first, the chosen panel showed compatibility to the results reported in the scientific literature (Pereira et al 2000, Morris et al 2002, Mukaratirwa & Gruys 2003, Araú-jo et al 2012, Mascarenhas et al 2014. In fact, the phenotypic profile presented by the authors to identify the TVT cells can be considered as an auxiliary method in the definitive diagnosis of the tumor.…”

Section: Discussionmentioning

confidence: 53%

“…From a clinical point of view, TVT cells have been usually characterized through markers such as vimentin, lysozyme, alpha-antitrypsin (Moore & Rosin 1986, Sandusky et al 1987, Mozos et al 1996, Marchal et al 1997, Pereira et al 2000, Morris et al 2002, Mukaratirwa & Gruys, 2003, Araújo et al 2012, Mascarenhas et al 2014. For this reason, these antibodies were firstly considered to characterize TVT primary cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Besides genital contact, TVT also may have an extra-genital localization (Pereira et al 2000, Rodrigues et al 2001, Albanese et al 2002, Siddle & Kaufman 2013. TVT usually is restricted to the points of origin and implantation of the disease, except in rare cases, around 2.5% (Pandey et al 1989), in which it invades adjacent tissues, lymphatic and/or blood circulation, and reaches distant areas such as lungs, liver, spleen, brain and other organs as metastatic deposits (Meuten 2002, Maxie 2007, Ganguly et al 2013, Strakova & Murchison 2014.…”

Section: Introductionmentioning

confidence: 99%

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Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor

et al. 2016

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RESUMO.-[Caracterização imunocitoquímica de culturas primárias de tumor venéreo transmissível canino.]Os anticorpos anti-vimentina, anti-lisozima, anti-alfa 1 antitripsina, anti-CD3 e anti-CD79α foram empregados para a caracterização de culturas primárias de tumor venéreo transmissível canino (TVT). Amostras para cultura primá-ria e imuno-histoquímica foram coletadas de oito cães com diagnóstico clínico e citológico de TVT. Para validar o resultado inmunocitoquímico nas culturas de TVT foi realizada a contagem de cromossomos. Para a análise estatística o teste de Mann-Whitney foi empregado a um nível de significância de p<0.05. As culturas e os tecidos de TVT apresentaram intensa reatividade para vimentina, moderada a leve para Lisozima, moderada para alfa-antitripsina e não houve marcação para CD3 e CD79α. Immunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. Immunocytochemical characterization of primary cell culture in transmissible venereal tumor o uso da immunocitoquímica para a caracterização de culturas de TVT. Assim, e devido ao fato de se observar semelhança entre a imunomarcação em células e tecidos, sugere-se que o uso desta técnica possa auxiliar na confirmação de culturas primárias do tumor, fato muito importante porque a utilização da cultura do tumor pode permitir o acesso a informação relevante sobre resposta potencial a um tratamento e conhecimento do comportamento biológico do tumor.TERMOS DE INDEXAÇÃO: Tumor venéreo transmissível canino, alfa 1 antitripsina, citogenética, lisozima, vimentina.

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Other Mesenchymal Tumors

Gross¹,

Ihrke²,

Walder³

et al. 2005

Skin Diseases of the Dog and Cat

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Immunohistochemical characterization of intraocular metastasis of a canine transmissible venereal tumor

Cited by 36 publications

References 9 publications

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor

Other Mesenchymal Tumors

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