Immunohistochemical characterization of axonal sprouting and reactive tissue changes after long-term implantation of a polyimide sieve electrode to the transected adult rat sciatic nerve

Klinge, Petra M.; Vafa, Morad Ali; Brinker, Thomas; Brandis, Almuth; Walter, G. F.; Stieglitz, Thomas; Samii, Madjid; Wewetzer, Konstantin

doi:10.1016/s0142-9612(00)00420-8

Cited by 59 publications

(46 citation statements)

References 44 publications

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“…An understanding of this organization is critical for the design of penetrating peripheral nerve electrodes. It has been previously observed that these types of electrodes are accompanied by a chronic inflammatory response including the recruitment of macrophages to the device interface (Klinge et al, 2001;Lago et al, 2007;Christensen et al, Fig. 5.…”

Section: Discussionmentioning

confidence: 89%

“…Specifically, the sciatic nerve is the peripheral nerve of choice when conducting animal studies involving nerve gap repair or peripheral nerve electrode implantation. With regard to electrode implantation, the rat sciatic nerve has been used extensively in experiments with nerve cuff (Jellema and Teepen, 1995;Rodrıguez et al, 2000;Vince et al, 2004;Vince et al, 2005a,b) and sieve or regenerating electrodes (Klinge et al, 2001;Ceballos et al, 2002;Lago et al, 2005;Castro et al, 2008;Kim et al, 2009;Lacour et al, 2009), whereas the cat sciatic nerve is also commonly used in nerve cuff electrode studies (Stein et al, 1977;Hoffer et al, 1981;Krarup and Loeb, 1988;Walter et al, 1995;Loeb and Peck, 1996;Grill and Mortimer, 2000;Romero et al, 2001) and has been used more frequently for experiments using penetrating peripheral nerve devices (Branner et al, 2001(Branner et al, , 2004Leventhal et al, 2006). To understand the changes that might occur in the underlying nerve after the implantation of these peripheral nerve devices, it is necessary to have knowledge of the basic structure and composition of the nerve under normal conditions.…”

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confidence: 99%

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Differences Exist in the Left and Right Sciatic Nerves of Naïve Rats and Cats

Christensen

Tresco

2015

The Anatomical Record

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The sciatic nerve of rats and cats is commonly used in experimental models of peripheral nerve injury and repair, as well as experiments involving peripheral nerve electrode implantation. In such experiments, morphometric parameters from the implanted nerve are commonly evaluated and compared to control values obtained from the contralateral nerves. However, this may not be an appropriate approach as differences may naturally exist in the structure of the two nerves owing to developmental or behavioral asymmetry. Additionally, in the cat, baseline values for standard morphometric parameters are not well established. In this study, we characterized fascicle area, fiber count, fiber density, fiber packing, mean g-ratio, and fiber diameter distributions in the rat and cat, as well as investigated the potential for naturally occurring sided differences in these parameters in both species. We also investigated whether animal age or location along the nerve influenced these parameters. We found that sided or left/right leg differences exist in some parameters in both the rat and the cat, calling into question the validity of using the contralateral nerve as a control. We also found that animal age and location along the nerve can make significant differences in the parameters tested, establishing the importance of using control nerves from age-and behaviorally matched animals whose morphometric parameters are collected and compared from the same location. Anat Rec, 298:1492Rec, 298: -1501Rec, 298: , 2015. V C 2015 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 89%

mentioning

confidence: 99%

Differences Exist in the Left and Right Sciatic Nerves of Naïve Rats and Cats

Christensen

Tresco

2015

The Anatomical Record

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“…1. The results of this qualitative analysis, which demonstrated that the microelectrode and control surfaces were not toxic to the human and mammal cells (Black, 1999), along with the widely accepted use of polymides and gold in biomedical devices (Schneider and Steiglitz, 2004;Liu et al, 2003;Kanno et al, 2002;Klinge et al, 2001), suggest that the Kapton and gold microelectrode array is suitable for use with human patients.…”

Section: Methodsmentioning

confidence: 95%

Micro-field evoked potentials recorded from the porcine sub-dural cortical surface utilizing a microelectrode array

Kitzmiller

Hansford

Fortin

et al. 2007

Journal of Neuroscience Methods

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A sub-dural surface microelectrode array designed to detect microfield evoked potentials has been developed. The device is comprised of an array of 350-micron square gold contacts, with bidirectional spacing of 150 microns, contained within a polyimide Kapton material. Cytotoxicity testing suggests that the device is suitable for use with animal and human patients. Implementation of the device in animal studies revealed that reliable evoked potentials could be acquired. Further work will be needed to determine how these microfield potentials, which demonstrate selectivity for one eye, relate to the distribution of the ocular dominance columns of the occipital cortex.

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“…Polyclonal anti-GFAP and olfactory marker protein (OMP) antibodies were from Dako (Dianova, Hamburg, Germany). The monoclonal 27C7 antibody, which was generated using viable neonatal rat Schwann cells as immunogen (Wewetzer et al 1996) is an established marker for non-myelin-forming Schwann cells (Weidner et al 1999;Klinge et al 2001;Vroemen and Weidner 2003) and has been characterized previously in detail (Wewetzer et al 1997).…”

Section: Antibodiesmentioning

confidence: 99%

“…After cryoprotection in 30% sucrose, the tissue was embedded in cryomatrix using liquid nitrogen-cooled isopenthane, as previously described (Klinge et al 2001). Frontal sections (12 m) were cut with a cryostat and mounted onto poly-L-lysine-coated object slides.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Cell surface expression of 27C7 by neonatal rat olfactory ensheathing cells in situ and in vitro is independent of axonal contact

Brandes

Khayami

Peck³

et al. 2011

Histochem Cell Biol

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Olfactory ensheathing cells (OECs) are Schwann cell-like glial cells of the olfactory system that promote neural regeneration after transplantation into the injured central nervous system. Compared to the closely related Schwann cells, however, the biological characterization of OECs has remained fragmentary. This is due to the fact that the expression of OEC-specific markers is subject to complex regulation and that intricate ultrastructural analysis is essential to determine their localization. The p75 neurotrophin receptor (p75(NTR)) as the prototype OEC marker, for example, is only expressed by a minor population of neonatal rat OECs in situ. The major population carries O4-positive axonal fragments on their surface after dissociation and up-regulates p75(NTR) during culturing (Wewetzer et al. in Glia 49:577-587, 2005). In the present study, we investigated whether the cell surface determinant 27C7, defined by a monoclonal antibody to Schwann cells, is also expressed by neonatal rat OECs in situ and in vitro. Primary cell suspensions of the olfactory bulb displayed 27C7 expression of both p75(NTR)-negative and p75(NTR)-positive OECs, while immature oligodendrocytes and astrocytes were devoid of any 27C7 labeling. This together with the finding that the intrafascicular OECs of the olfactory nerves in the mucosa expressed 27C7 but not p75(NTR), suggests that 27C7 was expressed by the entire OEC population in situ. Maintenance of OECs in the absence of olfactory neurons in organotypic slice culture up-regulated p75(NTR) but did not alter 27C7 expression. It is concluded that 27C7 unlike p75(NTR) is constitutively expressed by OECs and may, therefore, be a useful marker for characterization of neonatal OECs in situ and in vitro.

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Immunohistochemical characterization of axonal sprouting and reactive tissue changes after long-term implantation of a polyimide sieve electrode to the transected adult rat sciatic nerve

Cited by 59 publications

References 44 publications

Differences Exist in the Left and Right Sciatic Nerves of Naïve Rats and Cats

Differences Exist in the Left and Right Sciatic Nerves of Naïve Rats and Cats

Micro-field evoked potentials recorded from the porcine sub-dural cortical surface utilizing a microelectrode array

Cell surface expression of 27C7 by neonatal rat olfactory ensheathing cells in situ and in vitro is independent of axonal contact

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