Immunohistochemical assessment of oestrogen receptor and progesterone receptor distribution in biopsy samples of the bovine endometrium collected throughout the oestrous cycle

Boos, Alois; Meyer, William R.; Schwarz, Rudolf; Grünert, E

doi:10.1016/0378-4320(96)01492-3

Cited by 67 publications

(68 citation statements)

References 25 publications

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“…Our results have shown that in the non-pregnant cows on day 16, the oestrogen receptor mRNA and protein were expressed at moderate levels in deep glands and low to moderate levels in the luminal epithelium and superficial glands. This is consistent with a previous study in the cow which found a temporal and cell type difference in the expression of the oestrogen receptor (Boos et al 1996, Robinson et al 1998). There was a low level in the luminal epithelium throughout the cycle with peaks on days 1 and 15.…”

Section: Discussionsupporting

confidence: 93%

“…In contrast, the glandular epithelium and stroma oestrogen receptor expression was maximal at oestrus, when circulating oestradiol levels were high. Thus, it was concluded that oestrogen promotes uterine oestrogen receptor synthesis in most cell types, except in the luminal epithelium, which appeared not to be strongly influenced by ovarian steroid hormones (Boos et al 1996). In sheep, conversely, oestrogen receptor levels were low throughout the luteal phase, except in the deep glands (Wathes & Hamon 1993, Spencer & Bazer 1995.…”

Section: Discussionmentioning

confidence: 97%

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The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow

Robinson¹,

Mann²,

Ge³

et al. 1999

Journal of Endocrinology

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The expression of oxytocin receptor (OTR) in the uterine endometrium plays an important role in the initiation of luteolysis. During early pregnancy, the conceptus secretes interferon tau (IFN ) which inhibits OTR up-regulation and luteolysis. In this study, uterine horn cross sections were collected on day 16 from 15 pregnant cows (PREG), 9 uninseminated controls and 5 inseminated cows with no embryo present. The latter two groups had similar results and were combined to form a single non-pregnant (NP) group. The animals were given an oxytocin challenge shortly before tissue collection to assess prostaglandin F 2 (PGF 2 ) release through the measurement of the metabolite 13,14-dihydro-15-keto PGF 2 (PGFM). The mRNAs for OTR, oestrogen receptor (ER) and progesterone receptor (PR) were localised by in situ hybridisation. The results were quantified by optical density (OD) measurements from autoradiographs using image analysis. OTR protein was measured by autoradiography with iodinated oxytocin antagonist and ER and PR protein was detected by immunocytochemistry. The release of PGFM after the oxytocin challenge was significantly higher in the 14 NP cows (187% 15%) compared with the PREG group (131% 11%) (P<0·01). Low concentrations of OTR mRNA were localised to the luminal epithelium (LE) in 6 out of the 14 NP cows, of which 2 also expressed OTR protein, while OTR mRNA and protein were undetectable in all the pregnant animals. These results indicated that the sampling time coincided with the onset of the luteolytic mechanism in the NP cows. On day 16 ER mRNA was detectable in both the LE and glands of both PREG and NP animals. There were no differences in either ER mRNA or protein between NP and PREG samples. PR mRNA was moderately expressed in the caruncular stroma, with lower levels in the dense caruncular-like stroma and glands. There were no differences between PREG and NP animals. The expression of PR mRNA and protein in the deep glands was variable between animals. These results suggested that, in cows, the presence of an embryo suppressed the expression of OTR, but had no effect on the expression of the transcriptionally regulated ER on day 16.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 97%

The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow

Robinson¹,

Mann²,

Ge³

et al. 1999

Journal of Endocrinology

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“…PGE, thromboxane A2) to the site of trauma (Scenna et al, 2005, Ricciotti and. Uterine trauma with the subsequent release of endogenous PGF2α may result from reproductive procedures involving uterine manipulation (UM) (Wann andRandel, 1990, Velez et al, 1991), such as passage and placement of devices in the uterine lumen through the cervix for artificial insemination (AI) embryo transfer (ET) (Scenna et al, 2005, Koblischke et al, 2008 or the collection of endometrial biopsy (EB) (Baker et al, 1981, Boos et al, 1996. This stimulation of PGF2α release may disrupt the including early CL regression or insufficient endometrial conditioning.…”

Section: Introductionmentioning

confidence: 99%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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“…Em bovinos, a expressão dos ER uterinos apresenta alterações temporais e espaciais ao longo do ciclo estral porém, de maneira geral e simplista, a expressão dos ER uterinos é diretamente proporcional às concentrações circulantes de E2 e inversamente proporcional às concentrações circulantes de P4, estando portanto, mais expressos durante o proestro e estro do que durante a fase luteal (BOOS et al, 1996;ROBINSON et al, 2001;MARTIN et al, 2008 …”

Section: Atuação Da Ocitocina Na Luteóliseunclassified

“…Além disso, a fase de desenvolvimento folicular influenciou poucas variáveis relacionadas à luteólise. De forma geral, há consenso na literatura de que ocorre estímulo à expressão de ER e OTR durante o proestro e estro (MEYER et al, 1988;BOOS et al, 1996;ROBINSON et al, 2001), possivelmente em resposta às concentrações circulantes de E2. Assim, é provável que, na vaca em anestro, as concentrações circulantes de E2 sejam insuficientes para causar alterações significativas no endométrio, ou ainda que a pré-exposição à P4 seja necessária para que o E2 exerça seus efeitos clássicos mais relevantes sobre o endométrio, uma vez que em vacas e ovelhas ovariectomizadas, o tratamento com E2 elevou a resposta endometrial a injeções de OT apenas quando houve pré-exposição à P4 (HOMANICS & SILVIA, 1988;MANN et al, 2001;LAU et al, 1993;KAMINSKI et al, 1997;WALKER et al, 1997).…”

Section: Conclusõesunclassified

Efeito do tratamento com progesterona e do diâmetro folicular nas características histológicas e moleculares uterinas em vacas Nelore em anestro pós-parto

Filho¹,

de²

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Immunohistochemical assessment of oestrogen receptor and progesterone receptor distribution in biopsy samples of the bovine endometrium collected throughout the oestrous cycle

Cited by 67 publications

References 25 publications

The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow

The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Efeito do tratamento com progesterona e do diâmetro folicular nas características histológicas e moleculares uterinas em vacas Nelore em anestro pós-parto

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