Immunohistochemical and histochemical evidence for the presence of noradrenaline, serotonin and gamma-aminobutyric acid in chief cells of the mouse carotid body

Oomori, Yukio; Nakaya, Kazuhiro; Tanaka, Hiroshi; Iuchi, Hiromichi; Ishikawa, Katsushi; Satoh, Yoh-ichi; Ono, Kazuyuki

doi:10.1007/s004410050214

Cited by 16 publications

(24 citation statements)

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“…In contrast to the mouse [13,15], we noted a small number of chemoreceptor cells containing, mostly discrete, GABA immunoreactivities.…”

Section: Discussioncontrasting

confidence: 87%

“…To our knowledge, this demonstration is second to that in the mouse [13,15] that shows GABA in the mammalian carotid body. Distribution of the immunoreactivities we found in the cat is in overall accord with that reported in the mouse, where they were revealed both in the cytoplasm and in the dense-core vesicles [13].…”

Section: Discussionsupporting

confidence: 48%

“…Previous immunohistochemical study has shown GABA immunoreactivities in nearly all chemoreceptor cells of the mouse CB [15], where it coexists with catecholamine.…”

Section: Introductionmentioning

confidence: 91%

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GABA Immunoreactivity in Chemoreceptor Cells of the Cat Carotid Body.

Pokorski

Ohtani

1999

Acta Histochem. Cytochem

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“…In contrast to the mouse [13,15], we noted a small number of chemoreceptor cells containing, mostly discrete, GABA immunoreactivities.…”

Section: Discussioncontrasting

confidence: 87%

Section: Discussionsupporting

confidence: 48%

See 1 more Smart Citation

GABA Immunoreactivity in Chemoreceptor Cells of the Cat Carotid Body.

Pokorski

Ohtani

1999

Acta Histochem. Cytochem

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“…Abnormalties in glomus cells of the A/J mice were also apparent in immunocytochemistry for TH. The presence of TH in glomus cells is well known in several species (18,26,39) including mice (27), and, therefore, we used TH as a marker for glomus cells. We observed typical TH immunostaining in the carotid body of the DBA/2J mice.…”

Section: Discussionmentioning

confidence: 99%

Structural and functional differences of the carotid body between DBA/2J and A/J strains of mice

Yamaguchi

Balbir²,

Schofield

et al. 2003

Journal of Applied Physiology

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Shirahata. Structural and functional differences of the carotid body between DBA/2J and A/J strains of mice. J Appl Physiol 94: 1536-1542, 2003; 10.1152/japplphysiol. 00739.2002.-In a previous study, DBA/2J and A/J inbred mice showed extremely different hypoxic ventilatory responses, suggesting variations in their carotid bodies. We have assessed the morphological and functional differences of the carotid bodies in these mice. Histological examination revealed a clearly delineated carotid body only in the DBA/2J mice. Many typical glomus cells and glomeruli appeared in the DBA/2J but not in the A/J mice. The size of the carotid body in the DBA/2J and A/J mice was 6.3 Ϯ 0.5 ϫ 10 6 and 1.5 Ϯ 0.3 ϫ 10 6 m 3 , respectively. The area immunostained for tyrosine hydroxylase, an estimation of the glomus cell quantity, was four times larger in the DBA/2J mice than in the A/J mice. The individual data points in the DBA/2J mice segregated from those in the A/J mice. ACh increased intracellular Ca 2ϩ in most clusters (81%) of cultured carotid body cells from the DBA/2J mice, but only in 18% of clusters in the A/J mice. These data suggest that genetic determinants account for the strain differences in the structure and function of the carotid body. genetic determinants; morphometry; immunocytochemistry; intracellular calcium; hypoxic ventilatory response THE CAROTID BODY IS A major player in the ventilatory responses to hypoxia (7,11,12). During hypoxia, the neural output from the carotid body increases and reflexly modifies several variables in the respiratory system. A prominent response is an increase in ventilation, but the hypoxic ventilatory response (HVR) among individuals varies widely (9,38,40). Studies with twins and longitudinal studies with the same individuals indicate that genetic factors significantly contribute to these differences in healthy humans (5,19,20,25,35). Recently, Tankersley et al. (33,34) demonstrated differential ventilatory responses to hypoxia among several inbred strains of mice, which indicates that genetic determinants robustly influenced HVR. They showed that the DBA/2J mice demonstrated the highest HVR and the A/J mice the lowest HVR (34). These data suggested that genetically regulated differences exist in the system controlling the HVR. The differences may be in the carotid body, chemoreceptor afferent, or the central integration network for hypoxia.Previous studies have suggested some correlation between the size of the carotid body and its function. For example, small carotid bodies were found in victims of sudden infant death syndrome (4, 24) and congenital hypoventilation syndrome (6). In these patients, HVR appears to be blunted (16,28). On the other hand, hypersensitivity of the carotid body has been reported in subjects with mild hypertension (17,36,37), and hypertrophy of the carotid body was noted in subjects with established hypertension (13, 15). Similarly, hypersensitivity of the carotid body to hypoxia and hyperoxia and enlargemant of the carotid body have been shown in sp...

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“…According to studies with inhibitory neurotransmitters, it has been reported that dopamine is present at high concentrations in the glomus cells (Gonzalez et al 1994). Immunohistochemically, immunoreactivity for tyrosine hydroxylase (TH; EC1.14.16.2), the rate-limiting enzyme of catecholamine biosynthesis, was widely observed in the glomus cells (Wang et al 1991;Oomori et al 1994;Wang and Bisgard 2002). Additionally, it is reported that dopamine D 2 receptor mRNA is expressed in glomus cells of rat carotid body in a study using ISH (Czyzyk-Krzeska et al 1992b), and that immunoreactivity for dopamine D 2 receptor was observed in glomus cells and nerve fiber of human carotid body (Lazarov et al 2009).…”

mentioning

confidence: 99%

Short-term Hypoxia Increases Tyrosine Hydroxylase Immunoreactivity in Rat Carotid Body

Kato

Yamaguchi-Yamada

Yamamoto

2010

J Histochem Cytochem.

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S U M M A R Y Neurochemical and morphological changes in the carotid body are induced by chronic hypoxia, leading to regulation of ventilation. In this study, we examined the time courses of changes in immunohistochemical intensity for tyrosine hydroxylase (TH) and cellular volume of glomus cells in rats exposed to hypoxia (10% O 2 ) for up to 24 hr. Grayscale intensity for TH immunofluorescence was significantly increased in rats exposed to hypoxia for 12, 18, and 24 hr compared with control rats (p,0.05). The transectional area of glomus cells was not significantly different between experimental groups. The TH fluorescence intensity of the glomus cells exhibited a strong negative correlation with the transectional area in control rats (Spearman's ρ 5 20.70). This correlation coefficient decreased with exposure time, and it was lowest for the rats exposed to hypoxia for 18 hr (ρ 5 20.18). The histogram of TH fluorescence intensity showed a single peak in control rats. The peaks were gradually shifted to the right and became less pronounced in hypoxia-exposed rats, suggesting that a hypoxiainduced increase in TH immunoreactivity occurred uniformly in glomus cells. In conclusion, this study demonstrates that short-term hypoxia induces an increase in TH protein expression in rat carotid body glomus cells. (J Histochem Cytochem 58:839-846, 2010)

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Immunohistochemical and histochemical evidence for the presence of noradrenaline, serotonin and gamma-aminobutyric acid in chief cells of the mouse carotid body

Cited by 16 publications

References 0 publications

GABA Immunoreactivity in Chemoreceptor Cells of the Cat Carotid Body.

GABA Immunoreactivity in Chemoreceptor Cells of the Cat Carotid Body.

Structural and functional differences of the carotid body between DBA/2J and A/J strains of mice

Short-term Hypoxia Increases Tyrosine Hydroxylase Immunoreactivity in Rat Carotid Body

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