Immunohistochemical and biochemical evidence for the presence of serotonin in amphibian adrenal chromaffin cells

Delarue, Catherine; Leboulenger, F.; Morra, Mohamad; He´ry, Francis; Verhofstad, A.; Be´rod, A.; Denoroy, Luc; Pelletier, Georges; Vaudry, Hubert

doi:10.1016/0006-8993(88)90281-8

Cited by 47 publications

(21 citation statements)

References 48 publications

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“…In addition to catecholamines, other neurotransmitters that influence adrenocortical activity are produced in the adrenal medulla. The occurrence of serotonin (5-HT) in adrenal chromaffin cells has been demonstrated in the mouse (Ritzén et al, 1965), rat (Holzwarth and Brownfield, 1985;Verhofstad and Jonsson, 1983), and frog (Delarue et al, 1988). In contrast, human adrenomedullary cells do not contain this amine and its presence has only been detected in mast cells Lefebvre et al, 1992).…”

Section: Second Pathwaymentioning

confidence: 95%

Morphological and functional studies of the paracrine interaction between cortex and medulla in the adrenal gland

Bornstein

Ehrhart‐Bornstein

Scherbaum

1997

Microsc. Res. Tech.

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Section: Second Pathwaymentioning

confidence: 95%

Morphological and functional studies of the paracrine interaction between cortex and medulla in the adrenal gland

Bornstein

Ehrhart‐Bornstein

Scherbaum

1997

Microsc. Res. Tech.

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“…1). The presence of serotonin (5-HT) has been demonstrated by immunohistochemical and biochemical approaches in the adrenal gland of the frog, mouse, rat, and human (2)(3)(4)(5); 5-HT-like immunoreactivity (5-HT-LI) has been found in adrenochromaffin cells of frogs, mice, and rats (2)(3)(4)6). In the frog adrenal gland, 5-HT-LI is only detected in chromaffin tissue; whereas, in the rat adrenal gland, 5-HT-LI is also present in mast cells located in the wall of adrenal arterioles and throughout the cortex (7,8).…”

Section: Incubation Of Cells From the Human Adrenal Cortex With 5-ht mentioning

confidence: 99%

Production and Metabolism of Serotonin (5-HT) by the Human Adrenal Cortex: Paracrine Stimulation of Aldosterone Secretion by 5-HT

Lefebvre

Compagnon

Contesse

et al. 2001

The Journal of Clinical Endocrinology & Metabolism

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In the human adrenal cortex, serotonin (5-HT) is contained in mast-like cells, and we have shown that 5-HT stimulates aldosterone secretion, suggesting that 5-HT may control glomerulosa cells through a paracrine mechanism. Concurrently, the presence of 5-hydroxyindolacetic acid in human adrenocortical extracts indicates that 5-HT may be metabolized after local release by mast cells. The aim of the present study was to investigate in vitro the production and metabolism of 5-HT by the human adrenal cortex. Perifused adrenal slices released spontaneously detectable amounts of 5-HT (0.74 +/- 0.38 fmol/mg wet tissue.min). The mast cell-depleting drug compound 48/80 induced a burst of 5-HT secretion followed by a gradual increase in aldosterone production. Administration of the specific 5-HT(4) receptor antagonist GR 113808 (10(-6) M) did not affect compound 48/80-induced 5-HT release but abolished the stimulatory effect of compound 48/80 on aldosterone secretion, indicating that 5-HT released locally is responsible for a paracrine control of steroidogenesis. Incubation of cells from the human adrenal cortex with 5-HT (10(-5) M) provoked the formation of the 5-HT metabolite 5-hydroxytryptophol. The type A monoamine oxidase (MAO) inhibitor clorgyline (10(-6) M) suppressed the metabolism of 5-HT into 5-hydroxytryptophol. Immunocytochemical staining of cultured cells revealed the presence of a subpopulation of MAO-A-positive cells. Double labeling with an antiserum against chromogranin A showed that MAO-A was actually contained in chromaffin cells. Similarly, immunohistochemical staining of adrenal slices showed that MAO-A was expressed in chromaffin cells located both in the medulla and in intracortical rays. In conclusion, the present study shows that, in the human adrenal cortex, 5-HT, released by mast-cells, may stimulate aldosterone secretion in a paracrine manner. Our data also indicate that 5-HT is metabolized by MAO-A located in intracortical chromaffin cells.

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“…In addition to catecholamines, the chromaffin cells of different anuran [68,69,153] and mammalian (see [88] for references) species also contain serotonin. In mammals, several studies have attributed direct peripheral catecholamine-releasing effects to serotonin [73,74,86,236].…”

Section: Humoral Agentsmentioning

confidence: 99%

The adrenergic stress response in fish: control of catecholamine storage and release

Reid

Bernier

Perry

1998

Comparative Biochemistry and Physiology Part C: Pharmacology, T

241

191

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In fish, the catecholamine hormones adrenaline and noradrenaline are released into the circulation, from chromaffin cells, during numerous 'stressful' situations. The physiological and biochemical actions of these hormones (the efferent adrenergic response) have been the focus of numerous investigations over the past several decades. However, until recently, few studies have examined aspects involved in controlling/modulating catecholamine storage and release in fish. This review provides a detailed account of the afferent limb of the adrenergic response in fish, from the biosynthesis of catecholamines to the exocytotic release of these hormones from the chromaffin cells. The emphasis is on three particular topics: (1) catecholamine biosynthesis and storage within the chromaffin cells including the different types of chromaffin cells and their varying arrangement amongst species; (2) situations eliciting the secretion of catecholamines (e.g. hypoxia, hypercapnia, chasing); (3) cholinergic and non-cholinergic (i.e. serotonin, adrenocorticotropic hormone, angiotensin, adenosine) control of catecholamine secretion. As such, this review will demonstrate that the control of catecholamine storage and release in fish chromaffin cells is a complex processes involving regulation via numerous hormones, neurotransmitters and second messenger systems.

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Immunohistochemical and biochemical evidence for the presence of serotonin in amphibian adrenal chromaffin cells

Cited by 47 publications

References 48 publications

Morphological and functional studies of the paracrine interaction between cortex and medulla in the adrenal gland

Morphological and functional studies of the paracrine interaction between cortex and medulla in the adrenal gland

Production and Metabolism of Serotonin (5-HT) by the Human Adrenal Cortex: Paracrine Stimulation of Aldosterone Secretion by 5-HT

The adrenergic stress response in fish: control of catecholamine storage and release

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