Immunohistochemical Analysis Revealed a Correlation between Musashi-2 and Cyclin-D1 Expression in Patients with Oral Squamous Cells Carcinoma

Abstract

Aim: Musashi 2 (MSI2), which is an RNA-binding protein, plays a fundamental role in the oncogenesis of several cancers. The aim of this study is to investigate the expression of MSI2 in Oral Squamous Cell Carcinoma (OSCC) and evaluate its correlation to clinic-pathological variables and prognosis. Materials and Methods: A bioinformatic analysis was performed on data downloaded from The Cancer Genome Atlas (TCGA) database. The MSI2 expression data were analysed for their correlation with clinic-pathological and prognostic features. In addition, an immmunohistochemical evaluation of MSI2 expression on 108 OSCC samples included in a tissue microarray and 13 healthy mucosae samples was performed. Results: 241 patients’ data from TCGA were included in the final analysis. No DNA mutations were detected for the MSI2 gene, but a hyper methylated condition of the gene emerged. MSI2 mRNA expression correlated with Grading (p = 0.009) and overall survival (p = 0.045), but not with disease free survival (p = 0.549). Males presented a higher MSI2 mRNA expression than females. The immunohistochemical evaluation revealed a weak expression of MSI2 in both OSCC samples and in healthy oral mucosae. In addition, MSI2 expression directly correlated with Cyclin-D1 expression (p = 0.022). However, no correlation has been detected with prognostic outcomes (overall and disease free survival). Conclusions: The role of MSI2 expression in OSCC seems to be not so closely correlated with prognosis, as in other human neoplasms. The correlation with Cyclin-D1 expression suggests an indirect role that MSI2 might have in the proliferation of OSCC cells, but further studies are needed to confirm such results.

Small Molecule Palmatine Targeting Musashi-2 in Colorectal Cancer

“…Moreover, a total of 38 genes were shared in all of the three clones, with significant alterations of protein abundance and almost unchanged mRNA levels upon MSI2 depletion ( Figures 2D,E ). Consistent to previous study ( Ito et al, 2010 ; García-Alegría et al, 2016 ; Troiano et al, 2019 ), NUMBL (a close homologue of NUMB) and cyclin D1 (CCND1) were also included to MSI2 candidate targets in both MSI2 knockout clones KO3 and KO21 ( Figure 2D ), supporting the reliability of our strategy in the study. Nevertheless, some reported MSI2 targets ( Wang et al, 2015 ; Kudinov et al, 2016 ; Tsujino et al, 2019 ) exhibited unexpected change patterns after MSI2 depletion, for instance, targets (CDKN1A, AHR, and LIG1) with both changed protein and mRNA levels and genes (HMGA2, CD9, CDCP1, NFYA, and JAG1) with unaltered protein and increased mRNA level ( Figure 2F ).…”

Long non-coding RNA SLC16A1-AS1: its multiple tumorigenesis features and regulatory role in cell cycle in oral squamous cell carcinoma

“…We found the knockdown of SLC16A1-AS1 reduced the proliferation of OSCC cells, suggesting that SLC16A1-AS1 contributed to the progression of OSCC. Cyclin D1 has been found to play a crucial role in cell cycle regulation, including cell proliferation and growth, as well as tumor grade in OSCC samples [17,18]. In this study, SLC16A1-AS1 silencing induced cell cycle arrest in G0/G1 phase and inhibited the expression of cyclin D1, indicating that SLC16A1-AS1 might behave as an oncogene that influences OSCC proliferation through regulating cell cycle-related genes.…”

Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis

“…A positive correlation between cyclin D1 expression and stage III and IV tongue carcinogenesis has been reported. 44 The malignant tumors of various organs have shown abnormal expression of cyclin D1. 44 The expression of PCNA, a 36KD protein, generally increases from the mid G1 point to S phase and then starts to reduce from G2/M to G1 phase of the cell cycle.…”

“…44 The malignant tumors of various organs have shown abnormal expression of cyclin D1. 44 The expression of PCNA, a 36KD protein, generally increases from the mid G1 point to S phase and then starts to reduce from G2/M to G1 phase of the cell cycle. 45 PCNA expression was found to be higher in poorly differentiated squamous cell carcinoma than well differentiated squamous cell carcinoma of the oral cavity.…”