Immunohistochemical analysis of the expression of FATE/BJ-HCC-2 antigen in normal and malignant tissues

Yang, Xiao Ang; Dong, Xue; Qiao, Huan; Wang, Yue Dan; Peng, Ji Run; Li, Yan; Pang, Xue Wen; Tian, Chan; Chen, Wei Feng

doi:10.1038/labinvest.3700220

Cited by 15 publications

(18 citation statements)

References 39 publications

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“…The X-linked FATE1 gene encodes a transcript originally identified as highly expressed in testis (40) and subsequently shown to be a cancer-testis antigen expressed in hepatocellular carcinomas. Its expression is inversely correlated with their differentiation state (41,42). Transfection of fetal and adult testis expressed-1 (FATE1) increases and anti-FATE1 antibodies decreases cell proliferation in hepatocarcinoma cells (42).…”

Section: Mechanisms Of Transcriptional Regulation By Increased Sf-1 Dmentioning

confidence: 99%

Increased Steroidogenic Factor-1 Dosage Triggers Adrenocortical Cell Proliferation and Cancer

Doghman

Karpova

Rodrigues

et al. 2007

Molecular Endocrinology

202

192

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Steroidogenic factor-1 (SF-1/Ad4BP; NR5A1), a nuclear receptor transcription factor, has a pivotal role in adrenal and gonadal development in humans and mice. A frequent feature of childhood adrenocortical tumors is SF-1 amplification and overexpression. Here we show that an increased SF-1 dosage can by itself augment human adrenocortical cell proliferation through concerted actions on the cell cycle and apoptosis. This effect is dependent on an intact SF-1 transcriptional activity. Gene expression profiling showed that an increased SF-1 dosage regulates transcripts involved in steroid metabolism, the cell cycle, apoptosis, and cell adhesion to the extracellular matrix. Consistent with these results, increased SF-1 levels selectively modulate the steroid secretion profile of adrenocortical cells, reducing cortisol and aldosterone production and maintaining dehydroepiandrosterone sulfate secretion. As a model to understand the mechanisms of transcriptional regulation by increased SF-1 dosage, we studied FATE1, coding for a cancer-testis antigen implicated in the control of cell proliferation. Increased SF-1 levels increase its binding to a consensus site in FATE1 promoter and stimulate its activity through modulation of the recruitment of specific cofactors. On the other hand, sphingosine, which can compete with phospholipids for binding to SF-1, had no effect on the SF-1 dosage-dependent increase of adrenocortical cell proliferation and expression of the FATE1 promoter. In mice, increased Sf-1 dosage produces adrenocortical hyperplasia and formation of tumors expressing gonadal markers (Amh, Gata-4), which originate from the subcapsular region of the adrenal cortex. Gene expression profiling revealed that genes involved in cell adhesion and the immune response and transcription factor signal transducer and activator of transcription-3 (Stat3) are differentially expressed in Sf-1 transgenic mouse adrenals compared with wild-type adrenals. Our studies reveal a critical role for SF-1 dosage in adrenocortical tumorigenesis and constitute a rationale for the development of drugs targeting SF-1 transcriptional activity for adrenocortical tumor therapy.

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Section: Mechanisms Of Transcriptional Regulation By Increased Sf-1 Dmentioning

confidence: 99%

Increased Steroidogenic Factor-1 Dosage Triggers Adrenocortical Cell Proliferation and Cancer

Doghman

Karpova

Rodrigues

et al. 2007

Molecular Endocrinology

202

192

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“…nude mice bearing nci-H1299 xenografts were injected with 1.8x10 10 pfu of M13 wild-type (control) and ZT-1 clone phages through the tail vein. The distribution and specificity of the phages binding to different tissues were further verified by the titers of bound phages in Figure 2.…”

Section: Affinity Of Phage M13 To Nci-h1299 Cells and Lung Cancer Tismentioning

confidence: 99%

Screening and identification of a peptide specifically targeted to NCI-H1299 cells from a phage display peptide library

Zang²,

Lan³

et al. 2009

Mol Med Rep

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Abstract. ligands that are capable of binding to tumor cell surface biomarkers specifically used in the early diagnosis of cancer and targeted drug delivery in cancer chemotherapy have been extensively investigated. Phage display technology has been demonstrated to be a powerful tool in this field. In this study, the non-small cell lung cancer nci-H1299 and the normal lung small airway epithelial cell lines were used for subtractive screening in vitro with a phage display 12-peptide library. after three rounds of panning, there was an obvious enrichment in the phages specifically binding to the NCI-H1299 cells, and the output/input ratio of phages increased approximately 875-fold (from 0.4x10 4 to 3.5x10 6 ). a group of peptides capable of binding specifically to the NCI-H1299 cells was obtained, and the affinity of these peptides to bind to the targeted cells and tissues was studied. Through cell-based eliSa, immunocytochemical staining, immunohistochemical staining and immunofluorescence, an M13 phage was isolated and identified from the above screenings, and a synthetic peptide, ZT-1 (sequence QQMHlMSyaPgP), corresponding to the sequence of the surface protein of the M13 phage, was demonstrated to be capable of binding to the tumor cell surfaces of nci-H1299 and a549 cells and biopsy specimens, but not to normal lung tissue samples, other cancer cells, or non-tumor adjacent lung tissues. in conclusion, the peptide ZT-1 may be a potential candidate biomarker ligand that can be used for targeted drug delivery in lung cancer therapy.

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“…For each round of selection, 2×10 11 pfu of collected phages were used, and the panning intensity was increased, prolonging the phages incubation process with L-02 cells to 1.25 h, 1.5 h, and 2 h and shortening that with HepG2 cells to 45 min, 30 min, and 15 min in the second, third, and fourth rounds individually and increasing TBST washing times to 4 times, 6 times, and 8 times in the second, third, and fourth round individually.…”

Section: In Vitro Panningmentioning

confidence: 99%

Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library

Zhang

Zhang²,

Wang³

et al. 2007

Mol Med

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Ligands specific to cell surface receptors have been heavily investigated in cancer research. Phage display technology is a powerful tool in this field and may impact clinical issues including functional diagnosis and targeted drug delivery. In this study, a hepatocellular carcinoma cell line (HepG2) and a normal hepatocyte line (L-02) were used to carry out subtractive screening in vitro with a phage display-7 peptide library. After four rounds of panning, there was an obvious enrichment for the phages specifically binding to the HepG2 cells, and the output/input ratio of phages increased about 976-fold (from 0.3×10 -7 to 292.8×10 -7 ).A group of peptides capable of binding specifically to the hepatoma cells were obtained, and the affinity of these peptides to the targeting cells and tissues was studied. Through a cell-based ELISA, immunocytochemical staining, immunohistochemical staining, and immunofluorescence, the S1 phage and synthetic peptide HCBP1 (sequence FQHPSFI) were shown to bind to the tumor cell surfaces of two hepatoma cell lines and biopsy specimens, but not to normal hepatocytes, other different cancer cells, or nontumor liver tissues. In conclusion, the peptide HCBP1 may be a potential candidate for targeted drug delivery in therapy of hepatoma cancer.

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Immunohistochemical analysis of the expression of FATE/BJ-HCC-2 antigen in normal and malignant tissues

Cited by 15 publications

References 39 publications

Increased Steroidogenic Factor-1 Dosage Triggers Adrenocortical Cell Proliferation and Cancer

Increased Steroidogenic Factor-1 Dosage Triggers Adrenocortical Cell Proliferation and Cancer

Screening and identification of a peptide specifically targeted to NCI-H1299 cells from a phage display peptide library

Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library

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