Immunohistochemical analysis of synovial membranes from inflammatory and non-inflammatory arthritides: scarcity of CD5 positive B cells and IL2 receptor bearing T cells

Smith, Malcolm D.; O’Donnell, J. R.; Highton, John; Palmer, D. G.; Rozenbilds, M. A. M.; Roberts‐Thomson, Peter

doi:10.3109/00313029209063615

Cited by 41 publications

(25 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sections (4p thick) were cut with a microtome and placed onto glass slides coated with 2% 3-aminopropyl triethoxysilane in acetone. The method used for immunohistochemical labeling of synovial membranes has been previously published (22). All sections from the same patient were processed in the same run, and the first section of each series was stained with a standard laboratory hematoxylin and eosin stain for histologic comparison.…”

Section: Methodsmentioning

confidence: 99%

Effects of pulse methylprednisolone on inflammatory mediators in peripheral blood, synovial fluid, and synovial membrane in rheumatoid arthritis

Youssef

Haynes

Triantafillou

et al. 1997

Arthritis & Rheumatism

Self Cite

105

View full text Add to dashboard Cite

Objective. To establish whether the clinical efficacy of pulse methylprednisolone (MP; 1,000 mg intravenously) is related to the modulation of proinflammatory cytokines within the peripheral blood, synovial membrane, or synovial fluid compartments.Methods. Eighteen patients with active rheumatoid arthritis (RA) were studied. Peripheral blood (11 patients) and knee synovial fluid (9 patients, 10 knees) were obtained before and at 4 and 24 hours after MP therapy. Interleukin-lp (IL-lp), IL-8, and tumor necrosis factor a (TNFa) were measured by enzyme-linked immunosorbent assay and biologic assays; prostaglandin E, (PGE,) was measured by competitive radioimmunoassay. In 10 patients, arthroscopically directed synovial biopsies were obtained before and at 24 hours after treatment, at disease relapse (4 patients), and after retreatment (1 patient). Membranes were stained by immunohistochemical techniques with monoclonal antibodies against TNFa, IL-8, IL-lp, and the IL-1 receptor antagonist protein (IL-1Ra).Results. MP therapy was associated with a rapid (within 24 hours) and substantial decrease in the expression of TNFa in the lining and sublining regions of the synovial membrane, as well as substantial decreases

show abstract

Section: Methodsmentioning

confidence: 99%

Effects of pulse methylprednisolone on inflammatory mediators in peripheral blood, synovial fluid, and synovial membrane in rheumatoid arthritis

Youssef

Haynes

Triantafillou

et al. 1997

Arthritis & Rheumatism

Self Cite

105

View full text Add to dashboard Cite

show abstract

“…Using specific inhibitors, we determined that these path- ways are implicated in chemokine and MMP production and also in the induction of apoptosis of osteoblasts by B. abortus. The MAPK signaling cascade, an important group of signaling pathways, has been implicated in bacterial pathogenesis (19,44,47,48), and in this model, Brucella has not been the exception.…”

Section: Discussionmentioning

confidence: 99%

Brucella abortus Invasion of Osteoblasts Inhibits Bone Formation

et al. 2012

View full text Add to dashboard Cite

ABSTRACTOsteoarticular brucellosis is the most common presentation of the active disease in humans. Loss of bone is a serious complication of localized bacterial infection of bones or the adjacent tissue, and brucellosis proved not to be the exception. The skeleton is a dynamic organ system which is constantly remodeled. Osteoblasts are responsible for the deposition of bone matrix and are thought to facilitate the calcification and mineralization of the bone matrix, and their function could be altered under infectious conditions. In this article, we describe immune mechanisms wherebyBrucella abortusmay invade and replicate within osteoblasts, inducing apoptosis, inhibiting mineral and organic matrix deposition, and inducing upregulation of RANKL expression. Additionally, all of these mechanisms contributed in different ways to bone loss. These processes implicate the activation of signaling pathways (mitogen-activated protein kinases [MAPK] and caspases) involved in cytokine secretion, expression of activating molecules, and cell death of osteoblasts. In addition, considering the relevance of macrophages in intracellularBrucellasurvival and proinflammatory cytokine secretion in response to infection, we also investigated the role of these cells as modulators of osteoblast survival, differentiation, and function. We demonstrated that supernatants fromB. abortus-infected macrophages may also mediate osteoblast apoptosis and inhibit osteoblast function in a process that is dependent on the presence of tumor necrosis factor alpha (TNF-α). These results indicate thatB. abortusmay directly and indirectly harm osteoblast function, contributing to the bone and joint destruction observed in patients with osteoarticular complications of brucellosis.

show abstract

“…The technique used for immunohistochemical labeling of synovial membranes has been previously published (39). Briefly, sections were removed from storage, thawed at room temperature, washed in Tris saline and incubated in endogenous peroxidase block, which consisted of methanol containing 3% hydrogen peroxide, for 10 minutes.…”

Section: Arthroscopic Biopsies Synovial Membrane Samplesmentioning

confidence: 99%

Effects of pulse methylprednisolone on cell adhesion molecules in the synovial membrane in rheumatoid arthritis. Reduced E‐selectin and intercellular adhesion molecule 1 expression

Youssef

Triantafillou

Parker

et al. 1996

Arthritis & Rheumatism

Self Cite

View full text Add to dashboard Cite

Objective. To investigate the effects of a 1,000-mg intravenous pulse of methylprednisolone succinate (MP) on cell adhesion molecule expression on the synovial vascular endothelium in patients with rheumatoid arthritis (RA).Methods. Sequential arthroscopic biopsy samples were taken before and 24 hours after MP administration (10 patients) and at the time of RA flare (2 patients) and after retreatment with MP (1 patient). Immunoperoxidase staining for E-selectin (CD62E), P-selectin (CD62P), intercellular adhesion molecule 1 (ICAM-1; CD54) and platelet-endothelial cell adhesion molecule (PECAM; CD31) was performed, and the staining was quantified by color video image analysis.Results. MP caused a rapid (within 24 hours) and substantial decrease in the expression of E-selectin on the synovial vascular endothelium, with a smaller reduction in ICAM-1 expression on synovial vascular endothelium and the synovial lining. There were no similar effects on synovial membrane P-selectin or PECAM expression.Conclusion. A potential mechanism by which MP

show abstract

Immunohistochemical analysis of synovial membranes from inflammatory and non-inflammatory arthritides: scarcity of CD5 positive B cells and IL2 receptor bearing T cells

Cited by 41 publications

References 36 publications

Effects of pulse methylprednisolone on inflammatory mediators in peripheral blood, synovial fluid, and synovial membrane in rheumatoid arthritis

Effects of pulse methylprednisolone on inflammatory mediators in peripheral blood, synovial fluid, and synovial membrane in rheumatoid arthritis

Brucella abortus Invasion of Osteoblasts Inhibits Bone Formation

Effects of pulse methylprednisolone on cell adhesion molecules in the synovial membrane in rheumatoid arthritis. Reduced E‐selectin and intercellular adhesion molecule 1 expression

Contact Info

Product

Resources

About