Immunohistochemical analysis of orbicularis oris muscle fiber distribution at the philtrum in healthy infants

Dong, Chenbin; Zheng, Shan

doi:10.1016/j.ijporl.2015.10.005

Cited by 4 publications

(7 citation statements)

References 12 publications

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“…These limitations are imposed by lack of commercially available functional antibodies for FFPE tissues (Suriyonplengsaeng et al., ), and the possibility of overfixation that might mask the epitope (Ramos‐Vara, ) or result in strong non‐specific background staining, impeding validation by immunohistochemistry (IHC). However, a recent study has demonstrated that the use of commercially available antibodies, designed for use in fresh tissues, gives satisfactory results when used in the analysis of FFPE lip tissue (Dong & Zheng, ). For this reason, in future work we will validate our preliminary findings using images obtained by IHC.…”

Section: Discussionmentioning

confidence: 99%

“…Mounting evidence indicates that the structure of the upper lip is intimately associated with the OO muscle (Schiaffino & Reggiani, ) and demonstrates varying distribution of OO muscle fiber types along the upper lip (Dong & Zheng, ). Fara, and Gundlach & Pfeifer, showed increased disorganization of muscle fiber with the degree of clefting.…”

Section: Introductionmentioning

confidence: 99%

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Muscle fiber diameter assessment in cleft lip using image processing

et al. 2017

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The image processing on FFPE tissues resulted in easy quantification of MFD with finding of a smaller MFD on the medial side in males suggesting possible differences in orbicularis oris (OO) muscle between the two sexes in CL that warrants replication using larger number of cases. Moreover, this finding can aid subclinical phenotyping and potentially in the restoration of the anatomy and function of the upper lip.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Muscle fiber diameter assessment in cleft lip using image processing

et al. 2017

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“…The orbicularis oris muscle contains both slow and fast fibers. The slow fibers are more resistant against fatigue and have a low activation threshold, whereas the fast fibers are more prone to fatigue and have a high activation threshold . Both the superficial and deep orbicularis oris muscle are predominantly composed of fast fibers, but there is a slight difference in fiber type composition between the superficial (98% fast) and deep muscle layers pars peripheralis (95% fast) and pars marginalis (91% fast).…”

Section: Muscle Anatomy In the Normal And Cleft Lipmentioning

confidence: 99%

“…Both the superficial and deep orbicularis oris muscle are predominantly composed of fast fibers, but there is a slight difference in fiber type composition between the superficial (98% fast) and deep muscle layers pars peripheralis (95% fast) and pars marginalis (91% fast). This indicates that these muscles should be reconstructed separately during surgery …”

Section: Muscle Anatomy In the Normal And Cleft Lipmentioning

confidence: 99%

“…This indicates that these muscles should be reconstructed separately during surgery. 38 In a cleft lip, all facial muscle fibers lack their insertions in the midline and therefore have an abnormal orientation on one or both sides (uni/bilateral cleft, respectively) ( Figure 2). The orbicularis oris normally functions as a sphincter but, in a cleft lip, it pulls the two portions of the lip laterally leading to an imbalance between lip and tongue pressure.…”

Section: Muscle Anatomy In the Normal And Cleft Lipmentioning

confidence: 99%

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Tissue engineering strategies combining molecular targets against inflammation and fibrosis, and umbilical cord blood stem cells to improve hampered muscle and skin regeneration following cleft repair

Schreurs

Suttorp

Mutsaers

et al. 2019

Medicinal Research Reviews

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Cleft lip with or without cleft palate is a congenital deformity that occurs in about 1 of 700 newborns, affecting the dentition, bone, skin, muscles and mucosa in the orofacial region. A cleft can give rise to problems with maxillofacial growth, dental development, speech, and eating, and can also cause hearing impairment. Surgical repair of the lip may lead to impaired regeneration of muscle and skin, fibrosis, and scar formation. This may result in hampered facial growth and dental development affecting oral function and lip and nose esthetics. Therefore, secondary surgery to correct the scar is often indicated. We will discuss the molecular and cellular pathways involved in facial and lip myogenesis, muscle anatomy in the normal and cleft lip, and complications following surgery. The aim of this review is to outline a novel molecular and cellular strategy to improve musculature and skin regeneration and to reduce scar formation following cleft repair. Orofacial clefting can be diagnosed in the fetus through prenatal ultrasound screening and allows planning for the harvesting of umbilical cord blood stem cells upon birth. Tissue engineering techniques using these cord blood stem cells and molecular targeting of inflammation and fibrosis during surgery may promote tissue regeneration. We expect that this novel strategy improves both muscle and skin regeneration, resulting in better function and esthetics after cleft repair.

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Comprehensive histological investigation of age‐related changes in dermal extracellular matrix and muscle fibers in the upper lip vermilion

Gomi

Imamura

2020

Intern J of Cosmetic Sci

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OBJECTIVE: Few histological studies have directly examined agerelated changes within the lips, although non-invasive investigations of such changes are increasing. Therefore, this study aimed to provide histological and molecular data on age-dependent alterations in the vermilion. METHODS: Upper vermilion specimens from 15 female Caucasian cadavers (age range, 27-78 years) were investigated histologically or immunohistochemically. RESULTS: Histologically, age-dependent decreases in areas occupied by hyaluronan and collagenous fibres in the dermis of upper vermilion were demonstrated. Elastic fibre content varied widely between individuals. The area occupied by muscle fibres in the orbicularis oris muscle region within the vermilion also correlated negatively with age. Immunohistochemically, signals of four proteins were attenuated in vermilion from older individuals compared with young individuals: procollagen type I, hyaluronan synthase (HAS)1, myosin heavy chain (MYH)2 (a component of fast-twitch oxidative muscle fibres) and MYH7 (a component of slow-twitch muscle fibres). In contrast, signals of cell migration inducing hyaluronidase 1 (CEMIP) were intensified in vermilion from older individuals. No marked differences between young and older individuals were seen in procollagen type III, HAS2, HAS3, hyaluronidase (HYAL)1, HYAL2, MYH1 or MYH4. CONCLUSION: Age-dependent decreases of hyaluronan in the dermis of vermilion were prominent, possibly due to both the decrease in synthesis (HAS1) and the increase in degradation (CEMIP). Furthermore, age-dependent decreases in collagenous fibres and two types of muscle fibre in the vermilion were also identified histologically. Type I collagen, MYH2 and MYH7 appear to represent the molecules responsible for these respec

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Immunohistochemical analysis of orbicularis oris muscle fiber distribution at the philtrum in healthy infants

Cited by 4 publications

References 12 publications

Muscle fiber diameter assessment in cleft lip using image processing

Muscle fiber diameter assessment in cleft lip using image processing

Tissue engineering strategies combining molecular targets against inflammation and fibrosis, and umbilical cord blood stem cells to improve hampered muscle and skin regeneration following cleft repair

Comprehensive histological investigation of age‐related changes in dermal extracellular matrix and muscle fibers in the upper lip vermilion

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